Identification of cell-type-specific genes of Volvox carteri and characterization of their expression during the asexual life cycle

1991 ◽  
Vol 145 (1) ◽  
pp. 51-66 ◽  
Author(s):  
Lai-Wa Tam ◽  
David L. Kirk
Keyword(s):  
Life Cycle ◽  
Cell Type ◽  
Volvox Carteri ◽  
Cell Type Specific

scholarly journals Connectivity characterization of the mouse basolateral amygdalar complex

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Houri Hintiryan ◽  
Ian Bowman ◽  
David L. Johnson ◽  
Laura Korobkova ◽  
Muye Zhu ◽  
...  
Keyword(s):  
Granular Cell ◽  
Cell Types ◽  
Projection Neurons ◽  
Cell Type ◽  
Connectivity Map ◽  
Analysis Techniques ◽  
Domain Specific ◽  
Cell Type Specific ◽  
Unique Domain

AbstractThe basolateral amygdalar complex (BLA) is implicated in behaviors ranging from fear acquisition to addiction. Optogenetic methods have enabled the association of circuit-specific functions to uniquely connected BLA cell types. Thus, a systematic and detailed connectivity profile of BLA projection neurons to inform granular, cell type-specific interrogations is warranted. Here, we apply machine-learning based computational and informatics analysis techniques to the results of circuit-tracing experiments to create a foundational, comprehensive BLA connectivity map. The analyses identify three distinct domains within the anterior BLA (BLAa) that house target-specific projection neurons with distinguishable morphological features. We identify brain-wide targets of projection neurons in the three BLAa domains, as well as in the posterior BLA, ventral BLA, posterior basomedial, and lateral amygdalar nuclei. Inputs to each nucleus also are identified via retrograde tracing. The data suggests that connectionally unique, domain-specific BLAa neurons are associated with distinct behavior networks.

Cytometric Profiling of CD133+ Cells in Human Colon Carcinoma Cell Lines Identifies a Common core Phenotype and Cell Type-specific Mosaics

2013 ◽  
Vol 28 (3) ◽  
pp. 267-273 ◽  
Author(s):  
Marica Gemei ◽  
Rosa Di Noto ◽  
Peppino Mirabelli ◽  
Luigi Del Vecchio
Keyword(s):  
Colorectal Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Colon Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Cell Type ◽  
Carcinoma Cell Lines ◽  
Cell Type Specific

In colorectal cancer, CD133+ cells from fresh biopsies proved to be more tumorigenic than their CD133– counterparts. Nevertheless, the function of CD133 protein in tumorigenic cells seems only marginal. Moreover, CD133 expression alone is insufficient to isolate true cancer stem cells, since only 1 out of 262 CD133+ cells actually displays stem-cell capacity. Thus, new markers for colorectal cancer stem cells are needed. Here, we show the extensive characterization of CD133+ cells in 5 different colon carcinoma continuous cell lines (HT29, HCT116, Caco2, GEO and LS174T), each representing a different maturation level of colorectal cancer cells. Markers associated with stemness, tumorigenesis and metastatic potential were selected. We identified 6 molecules consistently present on CD133+ cells: CD9, CD29, CD49b, CD59, CD151, and CD326. By contrast, CD24, CD26, CD54, CD66c, CD81, CD90, CD99, CD112, CD164, CD166, and CD200 showed a discontinuous behavior, which led us to identify cell type-specific surface antigen mosaics. Finally, some antigens, e.g. CD227, indicated the possibility of classifying the CD133+ cells into 2 subsets likely exhibiting specific features. This study reports, for the first time, an extended characterization of the CD133+ cells in colon carcinoma cell lines and provides a “dictionary” of antigens to be used in colorectal cancer research.

Characterization of cell type-specific S100B expression in the mouse olfactory bulb

Cell Calcium ◽  
2021 ◽  
Vol 94 ◽  
pp. 102334
Author(s):  
Xin Su ◽  
Tamara Vasilkovska ◽  
Nicole Fröhlich ◽  
Olga Garaschuk
Keyword(s):  
Olfactory Bulb ◽  
Cell Type ◽  
Cell Type Specific

scholarly journals Identification and characterization of cell type-specific and ubiquitous chromatin regulatory structures in the human genome

PLoS Genetics ◽  
2005 ◽  
Vol preprint (2007) ◽  
pp. e136
Author(s):  
Hualin Xi ◽  
Hennady P Shulha ◽  
Jane M Lin ◽  
Teresa R Vales ◽  
Yutao Fu ◽  
...  
Keyword(s):  
Human Genome ◽  
Cell Type ◽  
Cell Type Specific ◽  
Identification And Characterization

scholarly journals Characterization of Cre recombinase  mouse lines enabling cell type‐specific targeting of postnatal intervertebral discs

2019 ◽  
Vol 234 (9) ◽  
pp. 14422-14431 ◽  
Author(s):  
Yixin Zheng ◽  
Xuejie Fu ◽  
Qingbai Liu ◽  
Shengqi Guan ◽  
Cunchang Liu ◽  
...  
Keyword(s):  
Cre Recombinase ◽  
Intervertebral Discs ◽  
Cell Type ◽  
Specific Targeting ◽  
Cell Type Specific ◽  
Mouse Lines

scholarly journals Multilayer OMICS characterization of APOE‐modulated isogenic hiPSCs elucidates cell‐type–specific mechanisms modified by APOE: A study of the IMI ADAPTED Consortium

2020 ◽  
Vol 16 (S2) ◽  
Author(s):  
Janina S. Ried ◽  
Marco Rocha Curado ◽  
María Eugenia Sáez ◽  
Lamiaa Bahnassawy ◽  
Heyne Lee ◽  
...  
Keyword(s):  
Cell Type ◽  
Cell Type Specific

scholarly journals Bulk Tissue Cell Type Deconvolution with Multi-Subject Single-Cell Expression Reference

2018 ◽  
Author(s):  
Xuran Wang ◽  
Jihwan Park ◽  
Katalin Susztak ◽  
Nancy R. Zhang ◽  
Mingyao Li
Keyword(s):  
Single Cell ◽  
Cell Types ◽  
Cellular Heterogeneity ◽  
Tissue Cell ◽  
Specific Gene ◽  
Rna Seq ◽  
Cell Type ◽  
Cell Type Specific ◽  
Cell Expression

AbstractWe present MuSiC, a method that utilizes cell-type specific gene expression from single-cell RNA sequencing (RNA-seq) data to characterize cell type compositions from bulk RNA-seq data in complex tissues. When applied to pancreatic islet and whole kidney expression data in human, mouse, and rats, MuSiC outperformed existing methods, especially for tissues with closely related cell types. MuSiC enables characterization of cellular heterogeneity of complex tissues for identification of disease mechanisms.

scholarly journals Subcellular sequencing of single neurons reveals the dendritic transcriptome of GABAergic interneurons

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Julio D Perez ◽  
Susanne tom Dieck ◽  
Beatriz Alvarez-Castelao ◽  
Georgi Tushev ◽  
Ivy CW Chan ◽  
...  
Keyword(s):  
Hippocampal Neurons ◽  
Large Population ◽  
Cell Types ◽  
Mrna Localization ◽  
Gabaergic Neurons ◽  
Local Translation ◽  
Single Neurons ◽  
Cell Type ◽  
Cell Type Specific

Although mRNAs are localized in the processes of excitatory neurons, it is still unclear whether interneurons also localize a large population of mRNAs. In addition, the variability in the localized mRNA population within and between cell-types is unknown. Here we describe the unbiased transcriptomic characterization of the subcellular compartments of hundreds of single neurons. We separately profiled the dendritic and somatic transcriptomes of individual rat hippocampal neurons and investigated mRNA abundances in the soma and dendrites of single glutamatergic and GABAergic neurons. We found that, like their excitatory counterparts, interneurons contain a rich repertoire of ~4000 mRNAs. We observed more cell type-specific features among somatic transcriptomes than their associated dendritic transcriptomes. Finally, using cell-type specific metabolic labelling of isolated neurites, we demonstrated that the processes of Glutamatergic and, notably, GABAergic neurons were capable of local translation, suggesting mRNA localization and local translation is a general property of neurons.

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