scholarly journals Robust Survival-Based RNA Interference of Gene Families Using in Tandem Silencing of Adenine Phosphoribosyltransferase

2020 ◽  
Vol 184 (2) ◽  
pp. 607-619 ◽  
Author(s):  
Robert G. Orr ◽  
Stephen J. Foley ◽  
Catherine Sherman ◽  
Isidro Abreu ◽  
Giulia Galotto ◽  
...  
Keyword(s):  
Rna Interference ◽  
Gene Families ◽  
Adenine Phosphoribosyltransferase

scholarly journals A New Opaque Variant of Maize by a Single Dominant RNA-Interference-Inducing Transgene

Genetics ◽  
2003 ◽  
Vol 165 (1) ◽  
pp. 387-397 ◽  
Author(s):  
Gregorio Segal ◽  
Rentao Song ◽  
Joachim Messing
Keyword(s):  
Rna Interference ◽  
Antisense Rna ◽  
Gene Families ◽  
Small Interfering Rnas ◽  
Dominant Phenotype ◽  
Main Protein ◽  
Protein Components ◽  
Storage Protein Genes ◽  
Zein Genes ◽  
Nutritional Imbalance

Abstract In maize, α-zeins, the main protein components of seed stores, are major determinants of nutritional imbalance when maize is used as the sole food source. Mutations like opaque-2 (o2) are used in breeding varieties with improved nutritional quality. However, o2 works in a recessive fashion by affecting the expression of a subset of 22-kD α-zeins, as well as additional endosperm gene functions. Thus, we sought a dominant mutation that could suppress the storage protein genes without interrupting O2 synthesis. We found that maize transformed with RNA interference (RNAi) constructs derived from a 22-kD zein gene could produce a dominant opaque phenotype. This phenotype segregates in a normal Mendelian fashion and eliminates 22-kD zeins without affecting the accumulation of other zein proteins. A system for regulated transgene expression generating antisense RNA also reduced the expression of 22-kD zein genes, but failed to give an opaque phenotype. Therefore, it appears that small interfering RNAs not only may play an important regulatory role during plant development, but also are effective genetic tools for dissecting the function of gene families. Since the dominant phenotype is also correlated with increased lysine content, the new mutant illustrates an approach for creating more nutritious crop plants.

scholarly journals Gene Silencing by RNA Interference in the White Rot Fungus Phanerochaete chrysosporium

2008 ◽  
Vol 74 (17) ◽  
pp. 5359-5365 ◽  
Author(s):  
Avi Matityahu ◽  
Yitzhak Hadar ◽  
Carlos G. Dosoretz ◽  
Paula A. Belinky
Keyword(s):  
Rna Interference ◽  
Gene Silencing ◽  
Phanerochaete Chrysosporium ◽  
Gene Families ◽  
White Rot ◽  
Transcriptional Unit ◽  
White Rot Fungus ◽  
Activity Levels ◽  
Protein Activity ◽  
Rnai Technique

ABSTRACT The effectiveness of RNA interference (RNAi) is demonstrated in the lignin-degrading fungus Phanerochaete chrysosporium. The manganese-containing superoxide dismutase gene (MnSOD1) was used as the target for RNAi. The plasmid constructed for gene silencing contained a transcriptional unit for hairpin RNA expression. Significantly lower MnSOD expression at both the mRNA and protein activity levels was detected in RNAi transformants. Furthermore, even though P. chrysosporium possesses three copies of the MnSOD gene, this RNAi construct was sufficient to decrease the enzymatic activity by as much as 70% relative to control levels. Implementation of the RNAi technique in P. chrysosporium provides an alternative genetic tool for studies of gene function, particularly of essential genes or gene families.

Lentivirus-mediated RNA interference reveals that a testis-specific gene,LM23,is essential for spermatogenesis inRattus norvegicus

2010 ◽  
Vol 34 (8) ◽  
pp. S11-S11
Author(s):  
Mei‑ling Liu ◽  
Meng‑chun Jia
Keyword(s):  
Rna Interference ◽  
Specific Gene

RNA Interference Technology

2005 ◽  
Author(s):  
Andrew Fire ◽  
Marshall Nirenberg
Keyword(s):  
Rna Interference

The oncolytic adenovirus Ad-iREP-2 utilizes p53-dependent rna interference for tumor-selective replication

2008 ◽  
Vol 46 (09) ◽  
Author(s):  
E Gürlevik ◽  
P Schache ◽  
L Zender ◽  
MP Manns ◽  
S Kubicka ◽  
...  
Keyword(s):  
Rna Interference ◽  
Oncolytic Adenovirus ◽  
Tumor Selective

The Uptake of Adenine by Isolated Human Platelet Membranes

1974 ◽  
Vol 32 (02/03) ◽  
pp. 457-464
Author(s):  
Paul C. French ◽  
Jan J. Sixma ◽  
Holm Holmsen
Keyword(s):  
Human Platelet ◽  
Facilitated Diffusion ◽  
Adenine Phosphoribosyltransferase ◽  
Ph Optimum ◽  
Intact Cells ◽  
Platelet Membranes ◽  
Group Translocation ◽  
Triton X

SummaryAdenine uptake into isolated platelet membranes had about the same Km (151 ± 21 • 9 nM) as uptake into intact cells (159 ± 21 nM) and was also competitively inhibited by papaverine and hypoxanthine. No uptake occurred at 0° and accumulated adenine was converted to AMP. AMP was not firmly bound to protein as judged by chromatography of triton X-100 solubilized membranes on Sephadex G25. The pH optimum for adenine uptake was at pH 5-5. Exogenous 5-phosphoribosyl-l-pyrophos- phate strongly stimulated uptake. These data may be explained by uptake of adenine by facilitated diffusion followed by conversion to AMP by adenine phosphoribosyltransferase but group translocation cannot be entirely excluded.

Inhibition of hepatitis B virus replication by RNA interference

2009 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Yun Gyu Park
Keyword(s):  
Hepatitis B Virus ◽  
Rna Interference ◽  
Hepatitis B ◽  
Virus Replication ◽  
B Virus

RNA interference and its clinical applications

2007 ◽  
Vol 148 (47) ◽  
pp. 2235-2240 ◽  
Author(s):  
Gyöngyi Munkácsy ◽  
Zsolt Tulassay ◽  
Balázs Győrffy
Keyword(s):  
Rna Interference ◽  
Clinical Applications

Az RNS-interferencia a poszttranszkripciós génelcsendesítés olyan formája, amelynek során rövid, specifikusan RNS-molekulák elnyomják a gének kifejeződésében kulcsszerepet játszó hírvivő RNS-ek működését. A sejtbe juttatott dupla szálú vagy rövid interferáló RNS-molekulák aktiválják az RNS-indukált elcsendesítő komplexet, amely a célgén hírvivő RNS-ét lebontja. A sejtek saját szabályozó mikro-RNS-molekulákkal is rendelkeznek, amelyeknek hírvivő RNS-e képes önmagával hajtűt képezni, amit a sejt dupla szálú RNS-ként értelmez. Az RNS-interferencia élettani működései közé tartozik a vírusok és a transzpozonok elleni védekezés, valamint a génkifejeződés szabályozása. Az RNS-interferencia nemcsak in vitro alkalmazható az egyes gének működésének vizsgálatára, hanem klinikai alkalmazásainak lehetőségei is megjelentek. Eddig vírusfertőzésekben, az időskori makuladegeneráció gátlására, a vér koleszterinszint-csökkentésére, daganatellenes és neurodegeneratív betegségek kezelésében alkalmazták. Az RNS-interferencia alkalmazását azonban nehezíti, hogy a megfelelő rövid interferáló RNS-molekulák tervezéséhez szükséges bioinformatikai algoritmusok nem tökéletesek; a szervezet szöveteibe való bejuttatásuk nehéz; illetve csak olyan esetekben alkalmazható, amelyekben átmeneti antagonista génelcsendesítő hatás és nem hosszú távú kezelés szükséges. Az alkalmazás legnagyobb előnye a jelentős specificitás, ami miatt mellékhatása is kevés. Az RNS-interferencia alapú kezelések megjelenése már a közeli jövőben várható.

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