High Hydrostatic Pressure as a Method to Reduce Microbial Contamination of Porcine Blood Plasma

2001 ◽  
Vol 7 (2) ◽  
pp. 117-121
Author(s):  
D. ParÉs ◽  
E. Saguer ◽  
M. ToldrÀ ◽  
C. Carretero
2001 ◽  
Vol 7 (2) ◽  
pp. 117-121 ◽  
Author(s):  
D. Pares ◽  
E. Saguer ◽  
M. Toldra ◽  
C. Carretero

The suitability of high hydrostatic pressure as an alternative method to produce porcine blood plasma with a sufficient microbial stability without affecting its functional properties was evaluated. The effects of high pressure on plasma microorganisms were highly dependent on processing temperature. Treatments of 15 min at 450 MPa carried out at 5 'C led to reductions of about 90% in microbial counts and to 20-50% decreases in the growth ability of the survivors. At 25 'C and 40 'C, the efficiency was increased to reduction values of 99.82 and 99.97%, respectively. The lowering of the growth capacity was about 50% at 25 'C and up to 80% at 40 'C. The most efficient treatment (450 MPa, for 15 min at 40 'C) did not lead to appreciable negative effects on the functional properties of heat-induced gels from porcine blood plasma.


2012 ◽  
Vol 29 (3) ◽  
pp. 409-414 ◽  
Author(s):  
Nolwennig Rivalain ◽  
Jean Roquain ◽  
Jean-Michel Boiron ◽  
Jean-Paul Maurel ◽  
Alain Largeteau ◽  
...  

2004 ◽  
Vol 52 (4) ◽  
pp. 479-487 ◽  
Author(s):  
Cs. Pribenszky ◽  
M. Molnár ◽  
S. Cseh ◽  
L. Solti

Cryoinjuries are almost inevitable during the freezing of embryos. The present study examines the possibility of using high hydrostatic pressure to reduce substantially the freezing point of the embryo-holding solution, in order to preserve embryos at subzero temperatures, thus avoiding all the disadvantages of freezing. The pressure of 210 MPa lowers the phase transition temperature of water to -21°C. According to the results of this study, embryos can survive in high hydrostatic pressure environment at room temperature; the time embryos spend under pressure without significant loss in their survival could be lengthened by gradual decompression. Pressurisation at 0°C significantly reduced the survival capacity of the embryos; gradual decompression had no beneficial effect on survival at that stage. Based on the findings, the use of the phenomena is not applicable in this form, since pressure and low temperature together proved to be lethal to the embryos in these experiments. The application of hydrostatic pressure in embryo cryopreservation requires more detailed research, although the experience gained in this study can be applied usefully in different circumstances.


2010 ◽  
Vol 37 (6) ◽  
pp. 641-645 ◽  
Author(s):  
Can-Xin XU ◽  
Chun WANG ◽  
Bing-Yang ZHU ◽  
Zhi-Ping GAO ◽  
Di-Xian LUO ◽  
...  

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