High-energy phase-contrast X-ray imaging using a two-crystal X-ray interferometer

For the extraction of the best possible X-ray diffraction data from macromolecular crystals, accurate positioning of the crystals with respect to the X-ray beam is crucial. In addition, information about the shape and internal defects of crystals allows the optimization of data-collection strategies. Here, it is demonstrated that the X-ray beam available on the macromolecular crystallography beamline P14 at the high-brilliance synchrotron-radiation source PETRA III at DESY, Hamburg, Germany can be used for high-energy phase-contrast microtomography of protein crystals mounted in an optically opaque lipidic cubic phase matrix. Three-dimensional tomograms have been obtained at X-ray doses that are substantially smaller and on time scales that are substantially shorter than those used for diffraction-scanning approaches that display protein crystals at micrometre resolution. Adding a compound refractive lens as an objective to the imaging setup, two-dimensional imaging at sub-micrometre resolution has been achieved. All experiments were performed on a standard macromolecular crystallography beamline and are compatible with standard diffraction data-collection workflows and apparatus. Phase-contrast X-ray imaging of macromolecular crystals could find wide application at existing and upcoming low-emittance synchrotron-radiation sources.

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On filtration for high-energy phase-contrast x-ray imaging

10.1117/12.2082354 ◽

2015 ◽

Cited By ~ 1

Author(s):

Christian Riess ◽

Ashraf Mohamed ◽

Waldo Hinshaw ◽

Rebecca Fahrig

Keyword(s):

Phase Contrast ◽

High Energy ◽

X Ray ◽

X Ray Imaging

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Designing high-resolution slow scan CCD-based TEM camera systems

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012936x ◽

1992 ◽

Vol 50 (2) ◽

pp. 946-947

Author(s):

James F. Mancuso ◽

William B. Maxwell ◽

Russell E. Camp ◽

Mark H. Ellisman

Keyword(s):

Fiber Optics ◽

Ccd Camera ◽

High Energy ◽

Phosphor Layer ◽

X Ray ◽

Light Production ◽

Camera Systems ◽

X Ray Imaging ◽

Electron Image ◽

Scintillating Fiber

The imaging requirements for 1000 line CCD camera systems include resolution, sensitivity, and field of view. In electronic camera systems these characteristics are determined primarily by the performance of the electro-optic interface. This component converts the electron image into a light image which is ultimately received by a camera sensor.Light production in the interface occurs when high energy electrons strike a phosphor or scintillator. Resolution is limited by electron scattering and absorption. For a constant resolution, more energy deposition occurs in denser phosphors (Figure 1). In this respect, high density x-ray phosphors such as Gd2O2S are better than ZnS based cathode ray tube phosphors. Scintillating fiber optics can be used instead of a discrete phosphor layer. The resolution of scintillating fiber optics that are used in x-ray imaging exceed 20 1p/mm and can be made very large. An example of a digital TEM image using a scintillating fiber optic plate is shown in Figure 2.

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Principles of Different X-ray Phase-Contrast Imaging: A Review

Applied Sciences ◽

10.3390/app11072971 ◽

2021 ◽

Vol 11 (7) ◽

pp. 2971

Author(s):

Siwei Tao ◽

Congxiao He ◽

Xiang Hao ◽

Cuifang Kuang ◽

Xu Liu

Keyword(s):

Biological Samples ◽

Phase Contrast ◽

Recent Progress ◽

Phase Contrast Imaging ◽

Contrast Imaging ◽

X Ray ◽

X Ray Imaging ◽

Internal Structures ◽

X Ray Absorption ◽

Made In

Numerous advances have been made in X-ray technology in recent years. X-ray imaging plays an important role in the nondestructive exploration of the internal structures of objects. However, the contrast of X-ray absorption images remains low, especially for materials with low atomic numbers, such as biological samples. X-ray phase-contrast images have an intrinsically higher contrast than absorption images. In this review, the principles, milestones, and recent progress of X-ray phase-contrast imaging methods are demonstrated. In addition, prospective applications are presented.

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Visualization Ability of Phase-Contrast Synchrotron-Based X-Ray Imaging Using an X-Ray Interferometer in Soft Tissue Tumors

Technology in Cancer Research & Treatment ◽

10.1177/15330338211010121 ◽

2021 ◽

Vol 20 ◽

pp. 153303382110101

Author(s):

Thet-Thet Lwin ◽

Akio Yoneyama ◽

Hiroko Maruyama ◽

Tohoru Takeda

Keyword(s):

Computed Tomography ◽

Soft Tissue ◽

Spatial Resolution ◽

Phase Contrast ◽

Soft Tissue Tumors ◽

X Ray ◽

3 Dimensional ◽

Computed Tomography Images ◽

X Ray Imaging ◽

X Ray Computed

Phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer provides high sensitivity and high spatial resolution, and it has the ability to depict the fine morphological structures of biological soft tissues, including tumors. In this study, we quantitatively compared phase-contrast synchrotron-based X-ray computed tomography images and images of histopathological hematoxylin-eosin-stained sections of spontaneously occurring rat testicular tumors that contained different types of cells. The absolute densities measured on the phase-contrast synchrotron-based X-ray computed tomography images correlated well with the densities of the nuclear chromatin in the histological images, thereby demonstrating the ability of phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer to reliably identify the characteristics of cancer cells within solid soft tissue tumors. In addition, 3-dimensional synchrotron-based phase-contrast X-ray computed tomography enables screening for different structures within tumors, such as solid, cystic, and fibrous tissues, and blood clots, from any direction and with a spatial resolution down to 26 μm. Thus, phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer shows potential for being useful in preclinical cancer research by providing the ability to depict the characteristics of tumor cells and by offering 3-dimensional information capabilities.

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