Development of a scanning tunneling microscope forin situexperiments with a synchrotron radiation hard-X-ray microbeam

2006 ◽  
Vol 13 (2) ◽  
pp. 216-220 ◽  
Author(s):  
Akira Saito ◽  
Junpei Maruyama ◽  
Ken Manabe ◽  
Katsuyuki Kitamoto ◽  
Koji Takahashi ◽  
...  
2011 ◽  
Vol 11 (4) ◽  
pp. 2873-2881 ◽  
Author(s):  
Akira Saito ◽  
Takehiro Tanaka ◽  
Yasumasa Takagi ◽  
Hiromasa Hosokawa ◽  
Hiroshi Notsu ◽  
...  

Hyomen Kagaku ◽  
2007 ◽  
Vol 28 (8) ◽  
pp. 453-458
Author(s):  
Akira SAITO ◽  
Koji TAKAHASHI ◽  
Yasumasa TAKAGI ◽  
Kazuhisa HANAI ◽  
Hiromasa HOSOKAWA ◽  
...  

1994 ◽  
Vol 107 (11) ◽  
pp. 3127-3131
Author(s):  
M. Maaloum ◽  
D. Chretien ◽  
E. Karsenti ◽  
J.K. Horber

We demonstrate that the scanning tunneling microscope can be used to obtain information about arrangement of tubulin subunits in the microtubule wall. Long rows of subunits with a periodicity of 3.8 +/- 0.4 nm were clearly visible in the images of microtubules. The separation between the rows of subunits was 4.8 +/- 0.4 nm. Close inspection of two images revealed another periodicity of 7.8 +/- 0.4 nm in the contour levels of the protofilaments. This indicates that alpha and beta tubulin monomers can be resolved. In these areas the monomers were arranged according to a ‘B-type’ lattice. Scanning tunneling microscope images confirm that the lateral contacts between tubulin monomers in adjacent protofilaments are compatible with a three-start, left-handed helix model. This study demonstrates that scanning tunneling microscopy can give direct information on the structure and organization of macromolecular assemblies and can complement the classical methods of electron microscopy and X-ray scattering.


1989 ◽  
Vol 28 (Part 2, No. 5) ◽  
pp. L854-L857 ◽  
Author(s):  
Kunio Nakajima ◽  
Sadao Aoki ◽  
Tamotsu Koyano ◽  
Eiji Kita ◽  
Akira Tasaki ◽  
...  

2004 ◽  
Vol 75 (6) ◽  
pp. 2149-2153 ◽  
Author(s):  
Takeshi Matsushima ◽  
Taichi Okuda ◽  
Toyoaki Eguchi ◽  
Masanori Ono ◽  
Ayumi Harasawa ◽  
...  

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