The high-resolution crystal structure of phosphatidylinositol 4-kinase IIβ and the crystal structure of phosphatidylinositol 4-kinase IIα containing a nucleoside analogue provide a structural basis for isoform-specific inhibitor design

2015 ◽  
Vol 71 (7) ◽  
pp. 1555-1563 ◽  
Author(s):  
Martin Klima ◽  
Adriana Baumlova ◽  
Dominika Chalupska ◽  
Hubert Hřebabecký ◽  
Milan Dejmek ◽  
...  

Phosphatidylinositol 4-phosphate (PI4P) is the most abundant monophosphoinositide in eukaryotic cells. Humans have four phosphatidylinositol 4-kinases (PI4Ks) that synthesize PI4P, among which are PI4K IIβ and PI4K IIα. In this study, two crystal structures are presented: the structure of human PI4K IIβ and the structure of PI4K IIα containing a nucleoside analogue. The former, a complex with ATP, is the first high-resolution (1.9 Å) structure of a PI4K. These structures reveal new details such as high conformational heterogeneity of the lateral hydrophobic pocket of the C-lobe and together provide a structural basis for isoform-specific inhibitor design.

Author(s):  
J. Jakana ◽  
M.F. Schmid ◽  
P. Matsudaira ◽  
W. Chiu

Actin is a protein found in all eukaryotic cells. In its polymerized form, the cells use it for motility, cytokinesis and for cytoskeletal support. An example of this latter class is the actin bundle in the acrosomal process from the Limulus sperm. The different functions actin performs seem to arise from its interaction with the actin binding proteins. A 3-dimensional structure of this macromolecular assembly is essential to provide a structural basis for understanding this interaction in relationship to its development and functions.


2007 ◽  
Vol 7 (1) ◽  
pp. 35 ◽  
Author(s):  
In-Kwon Kim ◽  
Min-Kyu Kim ◽  
Ji-Hye Kim ◽  
Hyung-Soon Yim ◽  
Sun-Shin Cha ◽  
...  

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138401 ◽  
Author(s):  
Md. Imtaiyaz Hassan ◽  
Abdul Waheed ◽  
Jeffery H. Grubb ◽  
Herbert E. Klei ◽  
Sergey Korolev ◽  
...  

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Hironori Takeda ◽  
Motoyuki Hattori ◽  
Tomohiro Nishizawa ◽  
Keitaro Yamashita ◽  
Syed T. A. Shah ◽  
...  

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e79687 ◽  
Author(s):  
Md. Imtaiyaz Hassan ◽  
Abdul Waheed ◽  
Jeffery H. Grubb ◽  
Herbert E. Klei ◽  
Sergey Korolev ◽  
...  

Author(s):  
H.-J. Ou ◽  
J. M. Cowley

Using the dedicate VG-HB5 STEM microscope, the crystal structure of high Tc superconductor of YBa2Cu3O7-x has been studied via high resolution STEM (HRSTEM) imaging and nanobeam (∽3A) diffraction patterns. Figure 1(a) and 2(a) illustrate the HRSTEM image taken at 10' times magnification along [001] direction and [100] direction, respectively. In figure 1(a), a grain boundary with strong field contrast is seen between two crystal regions A and B. The grain boundary appears to be parallel to a (110) plane, although it is not possible to determine [100] and [001] axes as it is in other regions which contain twin planes [3]. Following the horizontal lattice lines, from left to right across the grain boundary, a lattice bending of ∽4° is noticed. Three extra lattice planes, indicated by arrows, were found to terminate at the grain boundary and form dislocations. It is believed that due to different chemical composition, such structure defects occur during crystal growth. No bending is observed along the vertical lattice lines.


Author(s):  
Satoshi Uchida ◽  
Tae Woong Kim ◽  
Ludmila Cojocaru ◽  
Takashi Kondo ◽  
Hiroshi Segawa

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