Crystallization and preliminary X-ray diffraction analysis of AntE, a crotonyl-CoA carboxylase/reductase fromStreptomycessp. NRRL 2288
2014 ◽
Vol 70
(6)
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pp. 734-737
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AntE fromStreptomycessp. NRRL 2288 is a crotonyl-CoA carboxylase/reductase that catalyzes the reductive carboxylation of various α,β-unsaturated acyl-CoAs to provide the building block at the C7 position for antimycin A biosynthesis. Recombinant AntE expressed inEscherichia coliwas crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space groupI222 orI212121, with unit-cell parametersa= 76.4,b= 96.7,c= 129.6 Å, α = β = γ = 90.0°. A diffraction data set was collected at the KEK Photon Factory to 2.29 Å resolution.
2015 ◽
Vol 71
(2)
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pp. 171-174
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2016 ◽
Vol 72
(6)
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pp. 480-484
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2014 ◽
Vol 70
(5)
◽
pp. 600-603
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2012 ◽
Vol 68
(2)
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pp. 244-246
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