scholarly journals How my Experiences at the MRC-LMB led to the Structure of the Ribosome

2014 ◽  
Vol 70 (a1) ◽  
pp. C935-C935
Author(s):  
Thomas Steitz
Keyword(s):  
Data Collection ◽  
Molecular Biology ◽  
Atomic Structure ◽  
Structure And Function ◽  
Structural Basis ◽  
Asymmetric Unit ◽  
Carboxypeptidase A ◽  
Central Dogma ◽  
And Function ◽  
Resolution Data

Max Perutz's Dunham Lectures at Harvard in 1963, in which he showed the first atomic structure of a protein (myoglobin) that anyone in the room had ever seen, led to my working on the structure of carboxypeptidase A (CPA) (in the Lipscomb lab) before going to the MRC LMB in Cambridge. "Rapid" data collection on CPA in the mid-1960s was 5,000 reflections in a week, and now obtaining 2.7 Å resolution data on crystals with two 70S ribosomes in the asymmetric unit takes 5 minutes. Importantly, the LMB promoted creative and novel science because of its cooperative, interactive atmosphere where everyone interacted in the hall or over coffee, lunch or tea. This influenced how I have carried out science over the subsequent years. In the canteen, Crick, Brenner, Perutz, etc., would be interacting and talking with postdocs and students about asking important questions and solving scientific problems. It was a great place to learn, develop and use the most advanced methods in protein crystallography and apply them to explore the most interesting and significant questions in molecular biology. The importance of integrating structure and function in our research goals was made clear. My interactions in the Cambridge LMB led me to pursue the structural basis for understanding Crick's Central Dogma of Molecular Biology – DNA makes DNA makes RNA makes proteins. This resulted in our ultimately determining the structures of the ribosome and its various complexes.

scholarly journals Why Governments Should Invest More in Fundamental Research

2019 ◽  
Vol 1 (1) ◽  
pp. 1-3
Author(s):  
Venki Ramakrishnan ◽  
Mejd Alsari
Keyword(s):  
Molecular Biology ◽  
Nobel Prize ◽  
Public Engagement ◽  
Royal Society ◽  
Scientific Research ◽  
Group Leader ◽  
Structure And Function ◽  
Science And Engineering ◽  
Fundamental Research ◽  
And Function

Venkatraman ‘Venki’ Ramakrishnan is the President of The Royal Society and Group Leader at the MRC Laboratory of Molecular Biology. In 2009 he shared the Nobel Prize in Chemistry ‘for studies of the structure and function of the ribosome’. In this interview he explains why governments should invest more in basic scientific research rather than simply on applied science and engineering. He also discusses interdisciplinarity, collaborations, and public engagement.

scholarly journals Understanding the impacts of self-shuffling approach on structure and function of shuffled endoglucanase enzyme via MD simulations

2020 ◽  
Vol 45 (2) ◽  
Author(s):  
Aslı Yenenler ◽  
Umut Gerlevik ◽  
Ugur Sezerman
Keyword(s):  
Experimental Studies ◽  
Salt Bridge ◽  
Md Simulations ◽  
Structure And Function ◽  
Computational Approach ◽  
Intramolecular Interactions ◽  
Structural Basis ◽  
Functional Differences ◽  
Structural Differences ◽  
And Function

AbstractObjectiveWe identify the impacts of structural differences on functionality of EG3_S2 endoglucanase enzyme with MD studies. The results of previous experimental studies have been explained in details with computational approach. The objective of this study is to explain the functional differences between shuffled enzyme (EG3_S2) and its native counterpart (EG3_nat) from Trichoderma reseei, via Molecular Dynamics approach.Materials and methodsFor this purpose, we performed MD simulations along 30 ns at three different reaction temperatures collected as NpT ensemble, and then monitored the backbone motion, flexibilities of residues, and intramolecular interactions of EG3_S2 and EG3_nat enzymes.ResultsAccording to MD results, we conclude that EG3_S2 and EG3_nat enzymes have unique RMSD patterns, e.g. RMSD pattern of EG3_S2 is more dynamic than that of EG3_nat at all temperatures. In addition to this dynamicity, EG3_S2 establishes more salt bridge interactions than EG3_nat.ConclusionBy taking these results into an account with the preservation of catalytic Glu residues in a proper manner, we explain the structural basis of differences between shuffled and native enzyme via molecular dynamic studies.

scholarly journals Structure and Function of a Novel LD-Carboxypeptidase A Involved in Peptidoglycan Recycling

2013 ◽  
Vol 195 (24) ◽  
pp. 5555-5566 ◽  
Author(s):  
D. Das ◽  
M. Herve ◽  
M.-A. Elsliger ◽  
R. U. Kadam ◽  
J. C. Grant ◽  
...  
Keyword(s):  
Structure And Function ◽  
Carboxypeptidase A ◽  
And Function

scholarly journals Structural Basis of Redox Signaling in Photosynthesis: Structure and Function of Ferredoxin:thioredoxin Reductase and Target Enzymes

2004 ◽  
Vol 79 (3) ◽  
pp. 233-248 ◽  
Author(s):  
Shaodong Dai ◽  
Kenth Johansson ◽  
Myroslawa Miginiac-Maslow ◽  
Peter Schürmann ◽  
Hans Eklund
Keyword(s):  
Redox Signaling ◽  
Structure And Function ◽  
Structural Basis ◽  
And Function ◽  
Ferredoxin:Thioredoxin Reductase

Mucin gene structure and expression: protection vs. adhesion

1995 ◽  
Vol 269 (5) ◽  
pp. G613-G627 ◽  
Author(s):  
B. J. Van Klinken ◽  
J. Dekker ◽  
H. A. Buller ◽  
A. W. Einerhand
Keyword(s):  
Molecular Biology ◽  
Gene Structure ◽  
Lymphocyte Activation ◽  
Structure And Function ◽  
Lymphocyte Homing ◽  
Major Step ◽  
Molecular Analyses ◽  
Specific Receptors ◽  
Gene Structure And Expression ◽  
And Function

The cloning of mucin cDNAs brought about by the application of molecular biology and molecular analyses constitutes a major step in understanding mucin structure and function. Here two classes of mucins are described: epithelium-associated and endothelium/leukocyte-associated mucins, which have thus far been described separately in the literature. The epithelial mucins are generally believed to play a role in cytoprotection. The endothelial and leukocyte class of mucins are adhesion molecules involved in lymphocyte homing and lymphocyte activation or are part of the adhesion cascade that plays a role in the initiation of inflammation. Mucins in general contain many threonine and serine residues, which are extensively O-glycosylated. Due to this profound glycosylation, mucins have a filamentous conformation. By virtue of their extended filamentous, and often negatively charged, structure, mucins can act as a barrier protecting the cell. However, when an opposing cell has specific receptors for mucins, adhesion can override the barrier function. Therefore, mucins may be powerful two-edged swords: they are both protective and adhesive.

Atomic structure of the trypsin-A90720A complex: a unified approach to structure and function

1994 ◽  
Vol 1 (2) ◽  
pp. 113-117 ◽  
Author(s):  
Angela Y. Lee ◽  
Tim A. Smitka ◽  
Rosanne Bonjouklian ◽  
Jon Clardy
Keyword(s):  
Atomic Structure ◽  
Structure And Function ◽  
Unified Approach ◽  
And Function

scholarly journals UNGKAPAN MARAH DAN UNGKAPAN PUJIAN MASYARAKAT NIAS DI KAKI GUNUNG PADANG

2020 ◽  
Vol 8 (1) ◽  
pp. 10
Author(s):  
Faula Ismi ◽  
Ermanto Ermanto
Keyword(s):  
Data Collection ◽  
Social Structure ◽  
Social Function ◽  
Structure And Function ◽  
The People ◽  
The Social ◽  
Data Source ◽  
And Function ◽  
The Village ◽  
Descriptive Method

This study  aimed to determine the structure and social function of folklore legend naming Batunabontar Batang Natal district Mandailing Natal district. The method used in this research was descriptive method. The data source of this research was the people who inhabit Batunabontar village, Batang Natal District, Mandailing Natal District. The instrument used in this study was the researcher himself and the informant based on the understanding of the structure and social function of the naming legend of a place. Data collection techniques used in this study were observation by observation and to strengthen the data obtained, direct interviews were carried out to the authorities in research on the Social Structure and Function of the Legend of the Naming of Batunabontar Village, Batang Natal District, Mandailing Natal District. The results of this study indicate that the Social Structure and Function of Naming Legend of Batunabontar Village, Batang Natal District, Mandailing Natal District has a unique history and structure and function in the village. The uniqueness of this Batunabontar makes the writer want to know the importance of the structure and function of the Batunabontar in Batang Natal District, Mandailing Natal Regency.  

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