Production and optimization of cellulase from agricultural waste and its application in bioethanol production by simultaneous saccharification and fermentation

Purpose – The purpose of this paper is to optimize production of cellulase enzyme from agricultural waste by using Aspergillus fumigatus JCF. The study also aims at the production of bioethanol using cellulase and yeast. Design/methodology/approach – Cellulase production was carried out using modified Mandel’s medium. The optimization of the cellulase production was carried out using Plackett-Burman and Response surface methodology. Bioethanol production was carried out using simultaneous saccharification and fermentation. Findings – Maximum cellulase production at optimized conditions was found to be 2.08 IU/ml. Cellulase was used for the saccharification of three different feed stocks, i.e. sugar cane leaves, corn cob and water hyacinth. Highest amount of reducing sugar was released was 29.1 gm/l from sugarcane leaves. Sugarcane leaves produced maximum bioethanol concentration of 9.43 g/l out of the three substrates studied for bioethanol production. Originality/value – The present study reveals that by using the agricultural wastes, cellulase production can be economically increased thereby bioethanol production.

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Bioethanol production from lignocellulosic feedstock using aqueous ammonia pretreatment and simultaneous saccharification and fermentation (SSF): process development and optimization

10.31274/etd-180810-551 ◽

2010 ◽

Author(s):

Xuan Li

Keyword(s):

Simultaneous Saccharification And Fermentation ◽

Process Development ◽

Aqueous Ammonia ◽

Bioethanol Production ◽

Lignocellulosic Feedstock ◽

Ammonia Pretreatment ◽

Ssf Process ◽

Simultaneous Saccharification

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Cellulosic biofuel production using emulsified simultaneous saccharification and fermentation (eSSF) with conventional and thermotolerant yeasts

Biotechnology for Biofuels ◽

10.1186/s13068-021-02008-7 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Shannon M. Hoffman ◽

Maria Alvarez ◽

Gilad Alfassi ◽

Dmitry M. Rein ◽

Sergio Garcia-Echauri ◽

...

Keyword(s):

Simultaneous Saccharification And Fermentation ◽

Agricultural Waste ◽

Biofuel Production ◽

Cellulosic Biofuels ◽

Processing Times ◽

Cellulosic Biofuel ◽

Thermotolerant Yeasts ◽

Rapid Hydrolysis ◽

Dedicated Energy Crops ◽

Simultaneous Saccharification

Abstract Background Future expansion of corn-derived ethanol raises concerns of sustainability and competition with the food industry. Therefore, cellulosic biofuels derived from agricultural waste and dedicated energy crops are necessary. To date, slow and incomplete saccharification as well as high enzyme costs have hindered the economic viability of cellulosic biofuels, and while approaches like simultaneous saccharification and fermentation (SSF) and the use of thermotolerant microorganisms can enhance production, further improvements are needed. Cellulosic emulsions have been shown to enhance saccharification by increasing enzyme contact with cellulose fibers. In this study, we use these emulsions to develop an emulsified SSF (eSSF) process for rapid and efficient cellulosic biofuel production and make a direct three-way comparison of ethanol production between S. cerevisiae, O. polymorpha, and K. marxianus in glucose and cellulosic media at different temperatures. Results In this work, we show that cellulosic emulsions hydrolyze rapidly at temperatures tolerable to yeast, reaching up to 40-fold higher conversion in the first hour compared to microcrystalline cellulose (MCC). To evaluate suitable conditions for the eSSF process, we explored the upper temperature limits for the thermotolerant yeasts Kluyveromyces marxianus and Ogataea polymorpha, as well as Saccharomyces cerevisiae, and observed robust fermentation at up to 46, 50, and 42 °C for each yeast, respectively. We show that the eSSF process reaches high ethanol titers in short processing times, and produces close to theoretical yields at temperatures as low as 30 °C. Finally, we demonstrate the transferability of the eSSF technology to other products by producing the advanced biofuel isobutanol in a light-controlled eSSF using optogenetic regulators, resulting in up to fourfold higher titers relative to MCC SSF. Conclusions The eSSF process addresses the main challenges of cellulosic biofuel production by increasing saccharification rate at temperatures tolerable to yeast. The rapid hydrolysis of these emulsions at low temperatures permits fermentation using non-thermotolerant yeasts, short processing times, low enzyme loads, and makes it possible to extend the process to chemicals other than ethanol, such as isobutanol. This transferability establishes the eSSF process as a platform for the sustainable production of biofuels and chemicals as a whole.

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A Comparison of the Bioethanol Production from Suweg (Amorphophallus campanulatus) through Separate Hydrolysis and Fermentation as well as Simultaneous Saccharification and Fermentation Using Saccharomyces cerevisiae

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/1053/1/012036 ◽

2021 ◽

Vol 1053 (1) ◽

pp. 012036

Author(s):

H Hargono ◽

B Jos ◽

P Purwanto ◽

S Sumardiono ◽

MF Zakaria

Keyword(s):

Saccharomyces Cerevisiae ◽

Simultaneous Saccharification And Fermentation ◽

Bioethanol Production ◽

Separate Hydrolysis And Fermentation ◽

Simultaneous Saccharification

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Bioethanol Production of Cellulase Producing Bacteria from Soils of Agrowaste Field

Journal of Scientific Research ◽

10.3329/jsr.v13i2.50574 ◽

2021 ◽

Vol 13 (2) ◽

pp. 643-655

Author(s):

A. Thomas ◽

M. Laxmi ◽

A. Benny

Keyword(s):

Treatment Process ◽

Agricultural Waste ◽

Cellulase Production ◽

Cellulolytic Enzymes ◽

Bioethanol Production ◽

Ethanolic Fermentation ◽

Cellulose Bioconversion ◽

Congo Red Staining ◽

Pre Treatment ◽

And Staphylococcus Aureus

With decades of studies on cellulose bioconversion, cellulases have been playing an important role in producing fermentable sugars from lignocellulosic biomass. Copious microorganisms that are able to degrade cellulose have been isolated and identified. The present study has been undertaken to isolate and screen the cellulase producing bacteria from soils of agrowaste field. Cellulase production has been qualitatively analyzed in carboxy methylcellulose (CMC) agar medium after congo red staining and NaCl treatment by interpretation with zones around the potent colonies. Out of the seven isolates, only two showed cellulase production. The morphogical and molecular characterization revealed its identity as Escherichia coli and Staphylococcus aureus. The potential of organisms for bioethanol production has been investigated using two substrates, namely, paper and leaves by subjecting with a pre-treatment process using acid hydrolysis to remove lignin which acts as physical barrier to cellulolytic enzymes. Ethanolic fermentation was done using Saccharomyces cerevisiae for 24-48 h and then the bioethanol produced was qualitatively proved by iodoform assay. These finding proves that ethanol can be made from the agricultural waste and the process is recommended as a means of generating wealth from waste.

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