Bioethanol production from lignocellulosic feedstock using aqueous ammonia pretreatment and simultaneous saccharification and fermentation (SSF): process development and optimization

Seaweed processing waste has been used for bioethanol production through simultaneous saccharification and fermentation (SSF). SSF is commonly used for bioethanol production to shorten the process and to increase the yield of ethanol produced by Trichoderma reesei and Saccharomyces cerevisiae. The aim of this research was to obtain the best concentration of T. reesei and S. cerevisiae to produce bioethanol by SSF. The concentration of T. reesei and S. cerevisiae used was 0 (control), 5, 10, 15 and 20% (v/v). The SSF process was carried out by using shaking incubator at 35 °C and rotation of 150 rpm for 3 days. The untreated and hot water treated seaweed processing waste used in this study have moisture content values of 12.94±0.08% and 15.38±0.19%, ash content values of 16.72±0.08% and 18.39±0.19%, lignin content values of 15.38±0.11% and 12.74±0.38%, and cellulose content values of 26.92±0.57% and 34.57±0.81%, respectively. The result of SSF process of seaweed processing waste showed that different concentrations of T. reesei and S. cerevisiae (control, 5, 10, 15 and 20%) yielded significant effect (p<0.05) on the total reducing sugars and ethanol produced. The Duncan Multiple Range Test (DMRT) showed that the treatment 10% of T. reesei and S. cerevisiae concentration in the seaweed processing waste treated with hot water was the best treatment producing highest yield of ethanol.

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Bioethanol Production from Vineyard Waste by Autohydrolysis Pretreatment and Chlorite Delignification via Simultaneous Saccharification and Fermentation

Molecules ◽

10.3390/molecules25112606 ◽

2020 ◽

Vol 25 (11) ◽

pp. 2606

Author(s):

Lacrimioara Senila ◽

Eniko Kovacs ◽

Daniela Alexandra Scurtu ◽

Oana Cadar ◽

Anca Becze ◽

...

Keyword(s):

Simultaneous Saccharification And Fermentation ◽

Degradation Products ◽

Liquid Fraction ◽

Operating Conditions ◽

Solid Loading ◽

Sodium Chlorite ◽

Chemical Compositions ◽

Bioethanol Production ◽

Ssf Process ◽

Simultaneous Saccharification

In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF). The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol. The autohydrolysis of the vine-shoot wastes was carried out at 165 and 180 °C for 10 min residence time, and the resulted solid and liquid phases composition were analysed. The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products. The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process. The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h. The principal component analysis was used to identify the main parameters that influence the chemical compositions of vine-shoot waste for different varieties.

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Simultaneous Saccharification and Fermentation (SSF) of Cellulose from Lignocellulise for 2nd Bioethanol Production: A Review

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.512-515.464 ◽

2012 ◽

Vol 512-515 ◽

pp. 464-467

Author(s):

Yu Xiao Wang ◽

Xiao Chen Bian ◽

Lin Lin Zhou

Keyword(s):

Capital Investment ◽

Simultaneous Saccharification And Fermentation ◽

Process Efficiency ◽

Lignocellulosic Materials ◽

Bioethanol Production ◽

The Status ◽

Key Issues ◽

Increasing Process ◽

Ssf Process ◽

Simultaneous Saccharification

Lignocellulosic materials, a abundant and renewable resources in the world, can be utilized for 2nd bioethanol production. Simultaneous saccharification and fermentation (SSF) has been regarded as a promising process for bioethanol production. This article reviews the status of SSF process for bioethanol production and its challenges for reducing production cost and increasing process efficiency, and the key issues mainly including high loading of cellulose substrate, high temperature of SSF reaction and its corresponding thermo-tolerant microorganism as well as alleviation or elimination of inhibitors have been analyzed. At last future prospects for commercialization of the 2nd bioethanol production by lignocellulosic materials with low capital investment are stated.

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Bioethanol Production from Several Tropical Wood Species using Simultaneous Saccharification and Fermentation Processes

Wood Research Journal ◽

10.51850/wrj.2012.3.2.106-116 ◽

2017 ◽

Vol 4 (1) ◽

pp. 106-116

Author(s):

M. Daud ◽

Wasrin Syafii ◽

Khaswar Syamsu

Keyword(s):

Saccharomyces Cerevisiae ◽

Wood Species ◽

Simultaneous Saccharification And Fermentation ◽

Dry Mass ◽

Bioethanol Production ◽

Yeast Saccharomyces Cerevisiae ◽

Tropical Wood ◽

Kraft Process ◽

Ssf Process ◽

Simultaneous Saccharification

The study was conducted to determine the best method of hydrolysis (saccharification) and fermentation for bioethanol production using simultaneous saccharification and fermentation process. Three different tropical wood species namely gmelina wood (Gmelina sp.), pine wood (Pinus merkusii) and oil palm (Elaeis guineensis Jacq.) were pre-treated using kraft process and then converted into bioethanol using simultaneous saccharification and fermentation (SSF) processes. The pulp produced by kraft process was analized to determine their chemical properties before treatments. SSF was performed in 500 ml fermentors with total slurry of 200 ml. The substrate and nutrient media were autoclaved (121ºC and 20 min). The samples diluted to 2.5% (w/v) of total slurry were used as substrate. The enzyme preparation used commercial cellulase enzyme. The amount of cellulase added was 4 and 8% (w/w) of dry mass of samples. All SSF process was conducted by inoculating yeast Saccharomyces cerevisiae into fermentor in the amount of 10% (v/v) 1.5 x 109 CFU/cc. The SSF experiments run for 96 h, and the data were investigated periodically every 24 h. The results showed total sugar and reducing sugar tended to decrease with time of inoculation whereas ethanol concentration increases significantly. The growth of yeast Saccharomyces cerevisiae tended to incease in initial inoculation and decrease by the end of inoculation. The best method of hydrolysis (saccharification) and fermentation using SSF process for all tropical wood species tested were using cellulase 8% of dry mass (DM) and 10% (v/v) of Saccharomyces cerevisiae which produced bioethanol with concentration of 0.98; 0.57 and 0.51% for gmelina, pine and oil palm respectively and produced yields 11.21, 5.85 and 3.20%, in that order.

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A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq0804231n ◽

2008 ◽

Vol 14 (4) ◽

pp. 231-234 ◽

Cited By ~ 15

Author(s):

Svetlana Nikolic ◽

Ljiljana Mojovic ◽

Marica Rakin ◽

Dusanka Pejin ◽

Dragisa Savic

Keyword(s):

Simultaneous Saccharification And Fermentation ◽

Ethanol Concentration ◽

Microwave Treatment ◽

Ethanol Productivity ◽

Bioethanol Production ◽

Corn Meal ◽

Microwave Assisted ◽

Batch System ◽

Ssf Process ◽

Simultaneous Saccharification

A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation (SSF) of corn meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. An optimal power of microwaves of 80 W and the 5-min duration of the microwave treatment were selected by following the concentration of glucose released from the corn meal suspensions at hidromodul of 1:3 (corn meal to water ratio) in the liquefaction step. The results indicated that the microwave pretreatment could increase the maximum ethanol concentration produced in the SSF process for 13.4 %. Consequently, a significant increase of the ethanol productivity on substrate (YP/S), as well as the volumetric ethanol productivity (P) in this process, could be achieved.

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Production and optimization of cellulase from agricultural waste and its application in bioethanol production by simultaneous saccharification and fermentation

Management of Environmental Quality An International Journal ◽

10.1108/meq-07-2015-0128 ◽

2016 ◽

Vol 27 (1) ◽

pp. 22-35 ◽

Cited By ~ 2

Author(s):

Elsa Cherian ◽

M. Dharmendira Kumar ◽

G. Baskar

Keyword(s):

Design Methodology ◽

Simultaneous Saccharification And Fermentation ◽

Agricultural Waste ◽

Cellulase Production ◽

Reducing Sugar ◽

Bioethanol Production ◽

Corn Cob ◽

Content Type ◽

Optimized Conditions ◽

Simultaneous Saccharification

Purpose – The purpose of this paper is to optimize production of cellulase enzyme from agricultural waste by using Aspergillus fumigatus JCF. The study also aims at the production of bioethanol using cellulase and yeast. Design/methodology/approach – Cellulase production was carried out using modified Mandel’s medium. The optimization of the cellulase production was carried out using Plackett-Burman and Response surface methodology. Bioethanol production was carried out using simultaneous saccharification and fermentation. Findings – Maximum cellulase production at optimized conditions was found to be 2.08 IU/ml. Cellulase was used for the saccharification of three different feed stocks, i.e. sugar cane leaves, corn cob and water hyacinth. Highest amount of reducing sugar was released was 29.1 gm/l from sugarcane leaves. Sugarcane leaves produced maximum bioethanol concentration of 9.43 g/l out of the three substrates studied for bioethanol production. Originality/value – The present study reveals that by using the agricultural wastes, cellulase production can be economically increased thereby bioethanol production.

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