Developing a nano-biosensor for early detection of pancreatic cancer

Sensor Review ◽  
2021 ◽  
Vol 41 (1) ◽  
pp. 93-100
Author(s):  
Isra Rawashdeh1 ◽  
Mohamed Ghazi Al-Fandi ◽  
Yahia Makableh ◽  
Tasneem Harahsha
Keyword(s):  
Pancreatic Cancer ◽  
Early Detection ◽  
Dynamic Range ◽  
Electrochemical Techniques ◽  
Therapy Response ◽  
Differential Pulse ◽  
Content Type ◽  
Electrochemical Biosensing ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Purpose The purpose of this paper is to report on the development of a simple electrochemical nanobiosensor for early detection of pancreatic cancer. The nanobiosensor uses the newly emerged stable micro ribonucleic acid (miR-21) as a cancer-associated biomarker for diagnosis, prognosis or therapy response. Design/methodology/approach The biosensing practice consists of two main steps: capturing probe immobilization on a working electrode modified with multi-walled carbon nanotubes and gold nanoparticles (MWCNTs-AuNPs) and then sensing the miR-21 interaction electrochemically. Two electrochemical techniques, atomic force microscopy and Fourier-transform infrared spectroscopy, were applied for characterizations. Findings The nanobiosensor sensitivity exhibited satisfying results to miR-21 and demonstrated a wide dynamic range with a detection limit of just about 3.68 femtomolar using the source measure unit (SMU). Originality/value Researchers commonly use potentiostats to perform the differential pulse voltammetry (DPV) measurements for the electrochemical biosensing applications. In this study, the SMU was used to perform the DPV to detect the biomarker miR-21 using the MWCNTs-AuNPs screen-printed electrode as the electrochemical system.

Developing an electrochemical immunosensor for early diagnosis of hepatocellular carcinoma

Sensor Review ◽  
2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Abdulrahman Al-Shami ◽  
Rami Joseph Oweis ◽  
Mohamed Ghazi Al-Fandi
Keyword(s):  
Hepatocellular Carcinoma ◽  
High Sensitivity ◽  
Differential Pulse ◽  
Electrochemical Immunosensor ◽  
Content Type ◽  
Force Microscopy ◽  
Promising Tool ◽  
Atomic Force ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Purpose This paper aims to report on the development of a novel electrochemical amperometric immunosensor to diagnose early hepatocellular carcinoma (HCC) by detecting the Midkine (MDK) biomarker. Design/methodology/approach Anti-Midkine antibodies were immobilized covalently through carbodiimides chemistry on carbon screen-printed electrodes modified with carboxylated multi-walled carbon nanotubes. The development process was characterized using cyclic voltammetry, electrochemical impedimetric spectroscopy, Fourier transform infrared spectroscopy and atomic force microscopy. Differential pulse voltammetry was used to investigate the immunosensor performance in detecting MDK antigen within the concentration range of 1 pg/ml to 100 ng/ml. Findings MDK immunosensor exhibited high sensitivity and linearity with a detection limit of 0.8 pg/ml and a correlation coefficient of 0.99. The biosensor also demonstrated high selectivity, stability and reproducibility. Originality/value The developed MDK immunosensor could be a promising tool to diagnose HCC and reduce the number of related deaths.

A Sensitive and Selective Electrochemical Sensor for Sulfadimethoxine Based on Electropolymerized Molecularly Imprinted Poly (o-aminophenol) Film

2020 ◽  
Vol 16 (4) ◽  
pp. 413-420 ◽  
Author(s):  
Youyuan Peng ◽  
Qiaolan Ji
Keyword(s):  
Electrochemical Sensor ◽  
Polymer Film ◽  
Water Samples ◽  
Differential Pulse ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Long Term Stability ◽  
Multi Walled Carbon Nanotubes ◽  
Aquaculture Water ◽  
Walled Carbon Nanotubes

Background: As a broad-spectrum antibiotic of the sulfonamide family, Sulfadimethoxine (SDM) has been widely utilized for therapeutic and growth-promoting purposes in animals. However, the use of SDM can cause residual problems. Even a low concentration of SDM in the aquatic system can exert toxic effects on target organisms and green algae. Therefore, the quantitation of SDM residues has become an important task. Methods: The present work describes the development of a sensitive and selective electrochemical sensor for sulfadimethoxine based on molecularly imprinted poly(o-aminophenol) film. The molecular imprinted polymer film was fabricated by electropolymerizing o-aminophenol in the presence of SDM after depositing carboxylfunctionalized multi-walled carbon nanotubes onto a glassy carbon electrode surface. SDM can be quickly removed by electrochemical methods. The imprinted polymer film was characterized by cyclic voltammetry, differential pulse voltammetry and scanning electron microscopy. Results: Under the selected optimal conditions, the molecularly imprinted sensor shows a linear range from 1.0 × 10-7 to 2.0 × 10-5 mol L-1 for SDM, with a detection limit of 4.0 × 10-8 mol L-1. The sensor was applied to the determination of SDM in aquaculture water samples successfully, with the recoveries ranging from 95% to 106%. Conclusion: The proposed sensor exhibited a high degree of selectivity for SDM in comparison to other structurally similar molecules, along with long-term stability, good reproducibility and excellent regeneration capacity. The sensor may offer a feasible strategy for the analysis of SDM in aquaculture water samples.

Biosensors for Food Toxin Detection: Carbon Nanotubes and Graphene

2015 ◽  
Vol 1725 ◽  
Author(s):  
Bansi D. Malhotra ◽  
Saurabh Srivastava ◽  
Shine Augustine
Keyword(s):  
Carbon Nanotubes ◽  
Early Detection ◽  
Electrochemical Properties ◽  
Carbon Materials ◽  
Electrochemical Biosensor ◽  
Food Contamination ◽  
Multi Walled Carbon Nanotubes ◽  
Carbon Based Nanomaterials ◽  
Food Toxin ◽  
Walled Carbon Nanotubes

ABSTRACTThere is increased interest towards the application of carbon based nanomaterials to biosensors since these can be used to quickly detect presence of the toxins in food, agricultural and environmental systems. The accurate, faster and early detection of food toxins is presently very important for ensuring safety and shelf life of agricultural commodities resulting from food contamination. The carbon materials (CNTs) and recently discovered graphene have been predicted to be promising candidates in the development of electrochemical biosensor owing to their exceptionally large surface area and interesting electrochemical properties. We focus on some of the recent results obtained in our laboratories pertaining to the development of biosensors based on multi-walled carbon nanotubes and graphene for mycotoxin(aflatoxin ) detection.

The Application of Glassy Carbon Electrode Modified by Multi-Walled Carbon Nanotubes for the Fast Determination of Hydroquinone in Wastewater

2014 ◽  
Vol 955-959 ◽  
pp. 1160-1166
Author(s):  
Shao Hua Li ◽  
Ying Ying Zhao ◽  
Jun Qing Yang ◽  
Guo Yan Zhang ◽  
Ju Rui Qi
Keyword(s):  
Carbon Nanotubes ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Phosphate Buffer Solution ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Buffer Solution ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

A novel multi-walled carbon nanotubes (MWCNTs)-modified glassy carbon electrode (GCE) with excellent stability, repeatability and anti-interference was synthesized and it exhibited an electrocatalytic signal for hydroquinone (HQ) compared to bare GCE, which suggested that the presence of MWCNTs efficiently enhances electron transfer. Various parameters such as pH, modifier volume, and scan rate were optimized using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods. Under optimum conditions, linear responses for HQ in 0.1mol·L-1 phosphate buffer solution of pH 6.0 were obtained in the range of 5×10-6 mol·L-1 to 2×10-4 mol·L-1 with detection limits of 2.7×10-6 mol·L-1 (signal-to-noise ratio of 3). Such MWCNTs-modified GCE was successfully applied for the determination of hydroquinone in simulated water samples and can be developed for the the detection of HQ.

scholarly journals Electrochemical study of dsDNA on carbon nanotubes paste electrodes applying cyclic and differential pulse voltammetry

2013 ◽  
Vol 11 (3) ◽  
pp. 413-423 ◽  
Author(s):  
Constantina Serpi ◽  
Anastasios Voulgaropoulos ◽  
Stella Girousi
Keyword(s):  
Carbon Nanotubes ◽  
Stripping Voltammetry ◽  
Differential Pulse ◽  
Carbon Paste ◽  
Guanine Oxidation ◽  
Double Stranded Dna ◽  
Calf Thymus ◽  
Multi Walled Carbon Nanotubes ◽  
Pre Treatment ◽  
Walled Carbon Nanotubes

AbstractAbstract Carbon nanotubes paste electrodes (CNTPEs) in combination with adsorptive transfer stripping voltammetry are shown to be very suitable for the determination of calf thymus double-stranded DNA (dsDNA). The performance of three types of multi-walled carbon nanotubes paste electrodes (MWCNTPEs) is investigated. The effects of surface pre-treatment and accumulation conditions on the adsorption and electrooxidation of the dsDNA at MWCNTPEs are also described. The results indicate that the electroactivity inherent to carbon nanotubes/paste electrodes allows a large enhancement of the guanine oxidation signal compared to that obtained at the conventional carbon paste electrodes (CPEs). Moreover, the extent of the enhancement dependents on the type of MWCNTs incorporated into the paste. Based on the signal of guanine, under optimal conditions, very low levels of dsDNA can be detected following short accumulation times for all three types of MWCNTPEs (MWCNTPE1, MWCNTPE2, MWCNTPE3), with detection limits of 2.64 mg L−1, 2.02 mg L−1 and 1.46 mg L−1, respectively. Additionally, the dsDNA isolated from rat liver tissues is determined by use of the previously mentioned MWCNTPEs. Graphical abstract

BisGMA/EPDM/amine functionalised MWCNTs based nanocomposites

2015 ◽  
Vol 44 (5) ◽  
pp. 266-275 ◽  
Author(s):  
Ankita Pritam Praharaj ◽  
Dibakar Behera ◽  
Tapan Kumar Bastia ◽  
Arun Kumar Rout
Keyword(s):  
Water Absorption ◽  
Low Cost ◽  
Cost Effective ◽  
Point Of View ◽  
Content Type ◽  
Wide Range ◽  
Multi Walled Carbon Nanotubes ◽  
Thermal And Electrical Properties ◽  
Electrical Materials ◽  
Walled Carbon Nanotubes

Purpose – This paper aims to focus on the development and study properties of bisphenol-A glycidyldimethacrylate (BisGMA) and ethylene–propylene–diene monomer (EPDM) blend-based nanocomposites containing amine-functionalised multi-walled carbon nanotubes (MWCNT-NH2) as a compatibiliser. Design/methodology/approach – First, BisGMA was synthesised from epoxy and methacrylic acid followed by the amine functionalisation of MWCNTs. A novel two-roll milling technique was then conducted to prepare nanocomposite specimens with MWCNT-NH2 as compatibiliser. Effect of MWCNT-NH2 content on the mechanical, thermal, electrical, corrosive and water absorption properties of the nanocomposites was investigated and results have been reported. Findings – The results of the present work reveal that MWCNT-NH2 acts as a potential compatibiliser and nanofiller in BisGMA/EPDM blend-based nanocomposites. The authors report here that the nanocomposites exhibit improved mechanical, thermal and electrical properties with increased addition of MWCNT-NH2. Moreover, desirable results are obtained at 5 phr of nanofiller loading beyond which the properties deteriorate due to particle agglomeration. The nanocomposites display negligible corrosion and water absorption characteristics. Thus, the above fabricated nanocomposites with optimum compatibiliser content can serve as low-cost structural, thermal and electrical materials which can also be utilised in corrosive and moist environments. Research limitations/implications – The present investigation has come up with the successful and cost-effective fabrication of BisGMA/EPDM blend-based nanocomposites with optimum nanofiller/compatibiliser (MWCNT-NH2) content that can be used for a wide range of structural, thermal and electrical projects, as it is corrosion and moisture resistant. It is also the most durable from the mechanical point of view. Originality/value – The above nanocomposites have never been designed before.

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