In this paper, a sense-amplifier-based flip-flop (SAFF) suitable for low-power high-speed operation is proposed. With the employment of a new sense-amplifier stage as well as a new single-ended latch stage, the power and delay of the flip-flop is greatly reduced. A conditional cut-off strategy is applied to the latch to achieve glitch-free and contention-free operation. Furthermore, the proposed SAFF can provide low voltage operation by adopting MTCMOS optimization. Post-layout simulation results based on a SMIC 55 nm MTCMOS show that the proposed SAFF achieves a 41.3% reduction in the CK-to-Q delay and a 36.99% reduction in power (25% input data toggle rate) compared with the conventional SAFF. Additionally, the delay and the power are smaller than those of the master-slave flip-flop (MSFF). The power-delay-product of the proposed SAFF shows 2.7× and 3.55× improvements compared with the conventional SAFF and MSFF, respectively. The area of the proposed flip-flop is 8.12 μm2 (5.8 μm × 1.4 μm), similar to that of the conventional SAFF. With the employment of MTCMOS optimization, the proposed SAFF could provide robust operation even at supply voltages as low as 0.4 V.