Computational model of electrical stimulation of a retinal ganglion cell with hexagonally arranged electrodes

For the purpose of optogenetic prosthetics of the receptive field of the retinal ganglion cell, we have created a bicistronic genetic construct that carries genes of excitatory (channelorhodopsin2) and inhibitory (anionic channelorhodopsin) rhodopsins. A distinctive feature of this construct is the combination of two genes into one construct with the mutant IRES inserted between them, which ensures precise ratio of the expression levels of the first and second gene in each transfected cell. It was found that the illumination of the central part of transfected neuron with light with a wavelength of 470 nm causes the generation of action potentials in the cell. At the same time, light stimulation of the periphery of the neuron causes cessation of the generation of action potentials. Thus, we were able to simulate the ON-OFF interaction of the receptive field of the retinal ganglion cell using optogenetic methods. Theoretically, this construction can be used for optogenetic prosthetics of degenerative retina in case of its delivery to ganglion cells using lentiviral vectors.

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The effects of temperature changes on retinal ganglion cell responses to electrical stimulation

2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) ◽

10.1109/embc.2015.7320128 ◽

2015 ◽

Author(s):

Matias I. Maturana ◽

Nicholas V. Apollo ◽

David J. Garrett ◽

Tatiana Kameneva ◽

Hamish Meffin ◽

...

Keyword(s):

Electrical Stimulation ◽

Ganglion Cell ◽

Retinal Ganglion Cell ◽

Retinal Ganglion ◽

Temperature Changes ◽

Cell Responses ◽

Effects Of Temperature

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Transcorneal Electrical Stimulation Inhibits Retinal Microglial Activation and Enhances Retinal Ganglion Cell Survival After Acute Ocular Hypertensive Injury

Translational Vision Science & Technology ◽

10.1167/tvst.7.3.7 ◽

2018 ◽

Vol 7 (3) ◽

pp. 2 ◽

Cited By ~ 7

Author(s):

Lin Fu ◽

Frederic Fung ◽

Stefan Koinzer ◽

Amy Cheuk-Yin Lo ◽

Yau-Kei Chan ◽

...

Keyword(s):

Electrical Stimulation ◽

Ganglion Cell ◽

Cell Survival ◽

Retinal Ganglion Cell ◽

Microglial Activation ◽

Retinal Ganglion ◽

Transcorneal Electrical Stimulation ◽

Retinal Ganglion Cell Survival

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Spike-triggered average electrical stimuli as input filters for bionic vision – a perspective.

10.31219/osf.io/ty3kc ◽

2018 ◽

Cited By ~ 1

Author(s):

Daniel Rathbun ◽

Nima Ghorbani ◽

Hamed Shabani ◽

Eberhart Zrenner ◽

Zohreh Hosseinzadeh

Keyword(s):

White Noise ◽

Ganglion Cell ◽

Retinal Ganglion Cell ◽

Visual Information ◽

Cell Types ◽

Electrical Stimulus ◽

Retinal Ganglion ◽

Retinal Implant ◽

Modelling Framework ◽

Stimulation Of

Bionic retinal implants are gaining acceptance in the treatment of blindness from degenerative diseases including retinitis pigmentosa and macular degeneration. A current obstacle to the improved performance of such implants is the difficulty of comparing the results of disparate experiments. Another obstacle is the current difficulty in selectively activating the many different retinal ganglion cell types that are used as separate pathways for visual information to the brain. To address these obstacles, we propose a modelling framework based on white noise stimulation and reverse correlation.In this perspective, we first outline early developments in visual retinal physiology leading up to the implementation of white noise stimuli and spike-triggered averaging. We then review recent efforts to adapt the white noise method for electrical stimulation of the retina and some of the nuances of this approach. Based on such white noise methods, we describe a modelling framework whereby the effect of any arbitrary electrical stimulus on a ganglion cell’s neural code can be better understood. This framework should additionally disentangle the effects of stimulation on photoreceptor, bipolar cell and retinal ganglion cell – ultimately supporting selective stimulation of specific ganglion cell types for a more nuanced bionic retinal implant. Finally, we point to upcoming considerations in this rapidly developing domain of research.

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