A new block adaptation algorithm realizing rapid identification system

Author(s):  
K. Oh'Ishi ◽  
H. Kubota ◽  
M. Onoda
2016 ◽  
Vol 2016 ◽  
pp. 1-9
Author(s):  
Debra E. Huffman ◽  
Yulia M. Serebrennikova ◽  
Jennifer M. Smith ◽  
Alison Roth ◽  
Jack N. Lindon ◽  
...  

Optics based technologies are being advanced by many diagnostic companies around the globe. This resurgence is being driven by several factors including novel materials, enhanced computer power, nonlinear optics, and advances in algorithmic and statistical analysis. This study expands on a previous paper that evaluated the capability of a reagent-free optical profiling platform technology that used multiwavelength transmission spectroscopy to identify bacterial pathogens from pure culture. This study combines multiwavelength angular scattering with transmission based analysis into a single algorithm that will identify bacterial pathogens. Six predominant organisms,S. aureus, E. coli,K. pneumoniaeandP. aeruginosa,E. faecalis,and coagulase negativeStaphylococcus, were analyzed from a total of 753 clinical isolates received from three large community hospital systems. The bacterial identification method used for comparison in this study was the Vitek-2 (bioMerieux) which utilizes a biochemically based identification system. All of the clinical isolates received were blinded as to their identification until completion of the optical analysis. Sensitivities ranged from 87.7 to 94.6% with specificities ranging from 97.2 to 99.9% indicating that optical profiling is a powerful and exciting new technology that could be developed for the rapid identification of pathogens without the use of chemical reagents.


2017 ◽  
Vol 43 (10) ◽  
pp. 1565
Author(s):  
Xin-Yi WANG ◽  
Xian-Tao AI ◽  
Jun-Duo WANG ◽  
Ya-Jun LIANG ◽  
Zhao-Long GONG ◽  
...  

2008 ◽  
Vol 52 (No. 2) ◽  
pp. 49-53 ◽  
Author(s):  
N. Topic Popovic ◽  
R. Coz-Rakovac ◽  
I. Strunjak-Perovic

The available data concerning rapid identification of fish bacteria via commercial phenotypic tests demonstrate that there is no agreement regarding the choice of the tests. However, API 20E, an identification system for Enterobacteriaceae and other non-fastidious Gram-negative rods developed for clinical specimens, seems to be increasingly used for the identification of fish pathogens. In this review, adaptation of API 20E for fish bacterial isolates and its distinctiveness for fish bacteria was assessed. Some strains are wrongly identified because they are not included in the database of API 20E system. API 20E reactions should be compared with the diagnostic schemes based on reactions in conventional phenotypic tests. Due to their significance for fish health and impact on the aquaculture, and because of the need for their rapid identification, some important fish bacteria should be included in the API 20E system, such as <i>Yersinia ruckeri</i>, <i>Edwardsiella ictaluri</i>, <i>Vibrio anguillarum</i>.


2017 ◽  
Vol 2 (1) ◽  
pp. 12-20 ◽  
Author(s):  
Farah Zakiyah Rahmanti ◽  
Novita Kurnia Ningrum ◽  
Septian Enggar Sukmana ◽  
Prajanto Wahyu Adi

Malaria is one of the serious diseases that require rapid handling, otherwise it can lead to death. One of the causes of malaria parasites is plasmodium falciparum which can cause severe or fatal malaria. Handling a medical late can increase the risk of death. Therefore, it takes a rapid identification system with a high percentage of accuracy to reduce the risk of death. This research aims to build an identification system of plasmodium falciparum in thick blood film using Gray Level Co-occurrence Matrix (GLCM) and Support Vector Machine (SVM). The GLCM is used to get texture feature values such as contrast, correlations, energy, and homogeneity from images. Those values is processed and as an input of classification using SVM. The research result using SVM for accuracy value of  plasmodium falciparum identification can reach 93.33%.


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