scholarly journals Notice of Retraction: In Vitro Antibacterial Activity of Medicinal Herb Extracts against Five Drug-Resistant Streptococcus Suis Strains

Author(s):  
Zun-lai Sheng ◽  
Yan-hua Li ◽  
Ji-chang Li
2018 ◽  
Vol 5 ◽  
pp. 69-76
Author(s):  
Mamata Adhikari ◽  
Anil Kumar Sah ◽  
Dev Raj Joshi

Objectives: In order to investigate alternate therapeutic option, this study was carried out to assess the in vitro antibacterial activity of gel extract of Aloe barbadensis against multiple antibiotic resistant Pseudomonas aeruginosa isolated from wound specimens. Methods: A total of 180 different wound specimens collected in a hospital, were subjected to isolate and identify P. aeruginosa by cultural methods. Antibiotic susceptibility testing was done by Kirby- Bauer disc diffusion method to screen multidrug resistant isolates. A. barbadensis extracts were prepared using aqueous and organic solvents and their in vitro inhibitory action was evaluated by agar well diffusion methods. Results: Out of total, 38 (21.1%) of the wound specimens showed the occurrence of P. aeruginosa, among which 15 (39%) isolates were multi-drug resistant. Organic extracts of various concentrations (0.2 - 0.8 v/v %) inhibited 66.7% of MDR and all non-MDR (n = 23) P. aeruginosa with zone of inhibition ranging from 9.5 ±1.0 to 21.3 ± 2.2 mm but not by aqueous extract. A positive Pearson’s correlation (r=0.983) was found between antibacterial effect and concentrations of the extracts. The antibacterial activity of organic extracts was statistically associated with antibiotic resistance profile of the organism (p<0.05). Conclusion: Organic extracts of A. barbadensis revealed variable in vitro inhibitory action against both MDR and non-MDR P. aeruginosa isolated from wound specimens. Although further confirmation is needed, aloe gel extract may be applied as an alternate treatment option.


2012 ◽  
Vol 1 (6) ◽  
pp. 133-137 ◽  
Author(s):  
Rehnuma Sharmeen ◽  
Md Nazmul Hossain ◽  
Md Mahbubur Rahman ◽  
Md Javed Foysal ◽  
Md Faruque Miah

The studies were carried out to evaluate antibacterial activity of 35 aqueous herbal extracts against a total of 20 clinical Klebshiella sp. isolates. The maximum antibacterial activity was found as 90% in crude extracts of Syzygium aromaticum (leaf) and Citrus limon L. (fruit) followed by 85% in Spondias pinnata (leaf). Sensitivity of these isolates was also evaluated for eight commercial antibiotic discs following disc diffusion assay where most of the isolates found to develop resistance against multiple commercial antibiotics. 85% of isolates exhibited resistant to chloramphenicol and erythromycin and 80% were resiatant to sulfamethoxazole and cephradine. The isolates showed their resistance between 55-60 % to the other four antibiotic discs, viz; gentamycin, streptomycin, ciprofloxacin and azithromycin. Among 35 herbal extracts tested, 19 herbal extrats were found to possess antimicrobial activity in all multi-drug resistant isolates. Therefore these herbal extracts could be used in future direction as alternative therapeutic agents for the treatment of human diseases caused by Klebsiella sp. DOI: http://dx.doi.org/10.3329/icpj.v1i6.10534 International Current Pharmaceutical Journal 2012, 1(6): 133-137


2020 ◽  
Vol 8 (3) ◽  
pp. 332 ◽  
Author(s):  
Alessandra Oliva ◽  
Maria Claudia Miele ◽  
Massimiliano De Angelis ◽  
Silvia Costantini ◽  
Maria Teresa Mascellino ◽  
...  

Introduction: Intestinal colonization with multi-drug resistant (MDR) microorganisms is a consequence of antimicrobial-induced gut dysbiosis. Given the effect of probiotics in modulating gut microbiota, the aim of the study was to investigate whether the ingestion of high concentration multi-strain probiotic formulation would change the antibacterial activity of the feces against clinical strains of MDR microorganisms. The corresponding in vitro antibacterial activity was also investigated. Materials/Methods: The feces of healthy donors (n = 6) were analyzed before and after a 7-day dietary supplementation with a multi-strain probiotic formulation and tested against MDR microorganisms of clinical concern (carbapenem-resistant (CR), Klebsiella pneumoniae (CR-Kp), CR-Acinetobacter baumannii (CR-Ab), CR-Pseudomonas aeruginosa (CR-Pa), and methicillin-resistant Staphylococcus aureus (MRSA)). The tested MDR pathogens were cultured with decreasing concentrations of fecal water obtained before and after the treatment period. Furthermore, to corroborate the results obtained from the feces of healthy donors, the in vitro antibacterial activity of probiotic formulation (both whole probiotic (WP) and probiotic surnatant (PS)) against the same collection of MDR microorganisms was evaluated at different incubation times throughout the minimum bactericidal dilution and the corresponding serial silution number. Results: While before probiotic administration, the fecal water samples did not inhibit MDR microorganism growth, after supplementation, a reduced bacterial growth was shown. Accordingly, a noticeable in vitro activity of WP and PS was observed. Conclusions: Although preliminary, these experiments demonstrated that a specific multi-strain probiotic formulation exhibits in vitro antibacterial activity against MDR microorganisms of clinical concern. If confirmed, these results may justify the administration of probiotics as a decolonization strategy against MDR microorganisms.


Food Control ◽  
2006 ◽  
Vol 17 (11) ◽  
pp. 929-932 ◽  
Author(s):  
Stéphanie Dupont ◽  
Nola Caffin ◽  
Bhesh Bhandari ◽  
Gary A. Dykes

2011 ◽  
Vol 42 (3) ◽  
pp. 980-991 ◽  
Author(s):  
Moustafa A El-Nakeeb ◽  
Hamida M Abou-Shleib ◽  
Amal M Khalil ◽  
Hoda G Omar ◽  
Omar M El-Halfawy

Sign in / Sign up

Export Citation Format

Share Document