In vivo Detection of Lesion and Hemorrhage in Mouse Brain using Noninvasive Photoacoustic Imaging

Author(s):  
Sihua Yang ◽  
Da Xing ◽  
Diwu Yang ◽  
Yeqi Lao ◽  
Lvming Zeng ◽  
...  
2007 ◽  
Vol 364-366 ◽  
pp. 1123-1127
Author(s):  
Shi Hua Yang ◽  
Ye Qi Lao

The highlight of photoacosutic imaging (PAI) is a method that combines ultrasonic resolution with high contrast due to light absorption. Photoacoustic signals carry the information of the light absorption distribution of biological tissue, which is often related to its character of structure, physiological and pathological changes because of different physiology conditions in response to different light absorption coefficients. A non-invasive PAI system was developed and successfully acquired in vivo images of mouse brain. Based on the intrinsic PA signals from the brain, the vascular network and the detailed structures of the mouse cerebral cortex were clearly visualized. The ability of PAI monitoring of cerebral hemodynamics was also demonstrated by mapping of the mouse superficial cortex with and without drug stimulation. The extracted PA signals intensity profiles obviously testified that the cerebral blood flow (CBF) in the mouse brain was changed under the stimulation of acetazolamide (ACZ). The experimental results suggest that PAI can provide non-invasive images of blood flow changes, and has the potential for brain function detection.


2019 ◽  
Vol 12 (03) ◽  
pp. 1941004
Author(s):  
Dan Wu ◽  
Xinxin Zhang ◽  
Jian Rong ◽  
Huabei Jiang

In this study, we developed a novel photoacoustic imaging technique based on poly (ethyleneglycol)-coated (PEGylated) gold nanorods (PEG-GNRs) (as the contrast agent) combined with traditional Chinese medicine (TCM) acupuncture (as the auxiliary method) for quantitatively monitoring contrast enhancement in the vasculature of a mouse brain in vivo. This study takes advantage of the strong near-infrared absorption (peak at [Formula: see text][Formula: see text]nm) of GNRs and the ability to adjust the hemodynamics of acupuncture. Experimental results show that photoacoustic tomography (PAT) successfully reveals the optical absorption variation of the vasculature of the mouse brain in response to intravenous administration of GNRs and acupuncture at the Zusanli acupoint (ST36) both individually and combined. The quantitative measurement of contrast enhancement indicates that the composite contrast agents (integration of acupuncture and GNRs) would greatly enhance the photoacoustic imaging contrast. The quantitative results also have the potential to estimate the local concentration of GNRs and even the real-time effects of acupuncture.


2011 ◽  
Vol 66 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Alex X. Li ◽  
Mojmir Suchy ◽  
Chunhui Li ◽  
Joseph S. Gati ◽  
Susan Meakin ◽  
...  

2017 ◽  
Vol 31 (1) ◽  
pp. e3837 ◽  
Author(s):  
Jia Guo ◽  
Zhu Gang ◽  
Yanping Sun ◽  
Andrew Laine ◽  
Scott A. Small ◽  
...  

2001 ◽  
Vol 19 (5) ◽  
pp. 739-743 ◽  
Author(s):  
C. Testa ◽  
C. Casieri ◽  
R. Canese ◽  
G. Carpinelli ◽  
F. Podo ◽  
...  

2017 ◽  
Vol 28 (5) ◽  
pp. 2176-2183 ◽  
Author(s):  
Jing Lv ◽  
Ya Peng ◽  
Shi Li ◽  
Zhide Guo ◽  
Qingliang Zhao ◽  
...  

2017 ◽  
Vol 114 (21) ◽  
pp. 5343-5348 ◽  
Author(s):  
Qian Chen ◽  
Chao Liang ◽  
Xiaoqi Sun ◽  
Jiawen Chen ◽  
Zhijuan Yang ◽  
...  

Abnormal H2O2 levels are closely related to many diseases, including inflammation and cancers. Herein, we simultaneously load HRP and its substrate, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), into liposomal nanoparticles, obtaining a Lipo@HRP&ABTS optical nanoprobe for in vivo H2O2-responsive chromogenic assay with great specificity and sensitivity. In the presence of H2O2, colorless ABTS would be converted by HRP into the oxidized form with strong near-infrared (NIR) absorbance, enabling photoacoustic detection of H2O2 down to submicromolar concentrations. Using Lipo@HRP&ABTS as an H2O2-responsive nanoprobe, we could accurately detect the inflammation processes induced by LPS or bacterial infection in which H2O2 is generated. Meanwhile, upon systemic administration of this nanoprobe we realize in vivo photoacoustic imaging of small s.c. tumors (∼2 mm in size) as well as orthotopic brain gliomas, by detecting H2O2 produced by tumor cells. Interestingly, local injection of Lipo@HRP&ABTS further enables differentiation of metastatic lymph nodes from those nonmetastatic ones, based on their difference in H2O2 contents. Moreover, using the H2O2-dependent strong NIR absorbance of Lipo@HRP&ABTS, tumor-specific photothermal therapy is also achieved. This work thus develops a sensitive H2O2-responsive optical nanoprobe useful not only for in vivo detection of inflammation but also for tumor-specific theranostic applications.


2011 ◽  
Vol 23 (1) ◽  
pp. 37-48 ◽  
Author(s):  
Nobuyuki Okamura ◽  
Masanori Mori ◽  
Shozo Furumoto ◽  
Takeo Yoshikawa ◽  
Ryuichi Harada ◽  
...  

1991 ◽  
Vol 65 (04) ◽  
pp. 432-437 ◽  
Author(s):  
A W J Stuttle ◽  
M J Powling ◽  
J M Ritter ◽  
R M Hardisty

SummaryThe anti-platelet monoclonal antibody P256 is currently undergoing development for in vivo detection of thrombus. We have examined the actions of P256 and two fragments on human platelet function. P256, and its divalent fragment, caused aggregation at concentrations of 10−9−3 × 10−8 M. A monovalent fragment of P256 did not cause aggregation at concentrations up to 10−7 M. P256–induced platelet aggregation was dependent upon extracellular calcium ions as assessed by quin2 fluorescence. Indomethacin partially inhibited platelet aggregation and completely inhibited intracellular calcium mobilisation. Apyrase caused partial inhibition of aggregation. Aggregation induced by the divalent fragment was dependent upon fibrinogen and was inhibited by prostacyclin. Aggregation induced by the whole antibody was only partially dependent upon fibrinogen, but was also inhibited by prostacyclin. P256 whole antibody was shown, by flow cytometry, to induce fibrinogen binding to indomethacin treated platelets. Monovalent P256 was shown to be a specific antagonist for aggregation induced by the divalent forms. In–111–labelled monovalent fragment bound to gel-filtered platelets in a saturable and displaceable manner. Monovalent P256 represents a safer form for in vivo applications


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