Hemispherical photoacoustic imaging of myocardial infarction: in vivo detection and monitoring

Angiogenesis is crucial in tissue repair and prevents scar tissue formation following an ischemic event such as myocardial infarction. The ischemia induces formation of new capillaries, which have high expression of integrin αvβ3. [68Ga]Ga-NODAGA-E[(cRGDyK)]2 ([68Ga]Ga-RGD) is a promising PET-radiotracer reflecting angiogenesis by binding to integrin αvβ3. A Göttingen mini-pig underwent transient catheter-induced left anterior descending artery (LAD) occlusion for 120 min, and after 8 weeks was imaged on a Siemens mMR 3T PET/MR system. A large antero-septal infarction was evident by late gadolinium enhancement (LGE) on the short axis and 2–4 chamber views. The infarcted area corresponded to the area with high [68Ga]Ga-RGD uptake on the fused PET/MR images, with no uptake in the healthy myocardium. To support the hypothesis that [68Ga]Ga-RGD uptake reflects angiogenesis, biopsies were sampled from the infarct border and healthy myocardium. Expression of αvβ3 was evaluated using immunohistochemistry. The staining showed higher αvβ3 expression in the capillaries of the infarct border compared to those in the healthy myocardium. These initial data confirm in vivo detection of angiogenesis using [68Ga]Ga-RGD PET in a translational model, which overall support the method applicability when evaluating novel cardio-protective therapies.

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Canine Acute Myocardial Infarction In Vivo Detection by MRI with Gradient Echo Technique and Contribution of Gd-DOTA

Investigative Radiology ◽

10.1097/00004424-198809001-00054 ◽

1988 ◽

Vol 23 ◽

pp. S254-S257 ◽

Cited By ~ 6

Author(s):

ELISABETH SCHOUMAN-CLAEYS ◽

GUY FRIJA ◽

DIDIER REVEL ◽

DIDIER DOUCET ◽

ANNE-MARIE DONADIEU

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Gradient Echo ◽

In Vivo Detection

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H2O2-responsive liposomal nanoprobe for photoacoustic inflammation imaging and tumor theranostics via in vivo chromogenic assay

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1701976114 ◽

2017 ◽

Vol 114 (21) ◽

pp. 5343-5348 ◽

Cited By ~ 222

Author(s):

Qian Chen ◽

Chao Liang ◽

Xiaoqi Sun ◽

Jiawen Chen ◽

Zhijuan Yang ◽

...

Keyword(s):

Near Infrared ◽

Photoacoustic Imaging ◽

Photoacoustic Detection ◽

Chromogenic Assay ◽

In Vivo Detection ◽

Specificity And Sensitivity ◽

Great Specificity ◽

Liposomal Nanoparticles ◽

Theranostic Applications

Abnormal H2O2 levels are closely related to many diseases, including inflammation and cancers. Herein, we simultaneously load HRP and its substrate, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), into liposomal nanoparticles, obtaining a Lipo@HRP&ABTS optical nanoprobe for in vivo H2O2-responsive chromogenic assay with great specificity and sensitivity. In the presence of H2O2, colorless ABTS would be converted by HRP into the oxidized form with strong near-infrared (NIR) absorbance, enabling photoacoustic detection of H2O2 down to submicromolar concentrations. Using Lipo@HRP&ABTS as an H2O2-responsive nanoprobe, we could accurately detect the inflammation processes induced by LPS or bacterial infection in which H2O2 is generated. Meanwhile, upon systemic administration of this nanoprobe we realize in vivo photoacoustic imaging of small s.c. tumors (∼2 mm in size) as well as orthotopic brain gliomas, by detecting H2O2 produced by tumor cells. Interestingly, local injection of Lipo@HRP&ABTS further enables differentiation of metastatic lymph nodes from those nonmetastatic ones, based on their difference in H2O2 contents. Moreover, using the H2O2-dependent strong NIR absorbance of Lipo@HRP&ABTS, tumor-specific photothermal therapy is also achieved. This work thus develops a sensitive H2O2-responsive optical nanoprobe useful not only for in vivo detection of inflammation but also for tumor-specific theranostic applications.

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In vivo Detection of Lesion and Hemorrhage in Mouse Brain using Noninvasive Photoacoustic Imaging

2007 IEEE/ICME International Conference on Complex Medical Engineering ◽

10.1109/iccme.2007.4381893 ◽

2007 ◽

Author(s):

Sihua Yang ◽

Da Xing ◽

Diwu Yang ◽

Yeqi Lao ◽

Lvming Zeng ◽

...

Keyword(s):

Mouse Brain ◽

Photoacoustic Imaging ◽

In Vivo Detection

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106 In vivo quantification of absolute perfusion in mice with myocardial infarction by spin labelling magnetic resonance imaging

European Heart Journal ◽

10.1016/s0195-668x(03)93657-8 ◽

2003 ◽

Vol 24 (5) ◽

pp. 4

Author(s):

M NAHRENDORF

Keyword(s):

Magnetic Resonance Imaging ◽

Myocardial Infarction ◽

Magnetic Resonance ◽

Resonance Imaging ◽

Spin Labelling

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Scintigraphic Detection of Experimental Myocardial Infarction with 99mTc-2,3-Dimercaptosuccinic Acid

Nuklearmedizin ◽

10.1055/s-0038-1624263 ◽

1984 ◽

Vol 23 (06) ◽

pp. 317-319

Author(s):

J. Novák ◽

Y. Mazurová ◽

J. Kubíček ◽

J. Yižd’a ◽

P. Kafka ◽

...

Keyword(s):

Myocardial Infarction ◽

Coronary Artery ◽

Intravenous Administration ◽

Experimental Myocardial Infarction ◽

Myocardial Infarctions ◽

Clinical Use ◽

Scintigraphic Imaging ◽

Tissue Samples ◽

Scintigraphic Finding

SummaryAcute myocardial infarctions were produced by ligature of the left frontal descending coronary artery in 9 dogs. The possibility of scintigraphic imaging with 99mTc-DMSA 4 hrs after intravenous administration was studied. The infarctions were 4, 24 and 48 hrs old. The in vivo scan was positive in only one dog with a 4-hr old infarction. The in vivo scans were confirmed by the analysis of the radioactivity in tissue samples. The accumulation of the radiopharmaceutical increased slightly in 48-hr old lesions; however, this increase was not sufficient for a positive scintigraphic finding. Thus, we do not recommend 99mTc-DMSA for clinical use in acute lesions.

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Clinical Trials in Thrombosis: Secondary Prevention of Myocardial Infarction

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647910 ◽

1976 ◽

Vol 35 (01) ◽

pp. 049-056 ◽

Cited By ~ 4

Author(s):

Christian R Klimt ◽

P. H Doub ◽

Nancy H Doub

Keyword(s):

Myocardial Infarction ◽

Secondary Prevention ◽

Case Control ◽

Therapeutic Effects ◽

Case Control Studies ◽

Definitive Answer ◽

Inhibition Of Platelet Aggregation ◽

Prospective Trials

SummaryNumerous in vivo and in vitro experiments, investigating the inhibition of platelet aggregation and the prevention of experimentally-induced thrombosis, suggest that anti-platelet drugs, such as aspirin or the combination of aspirin and dipyridamole or sulfinpyrazone, may be effective anti-thrombotic agents in man. Since 1971, seven randomized prospective trials and two case-control studies have been referenced in the literature or are currently being conducted, which evaluate the effects of aspirin, sulfinpyrazone, or dipyridamole in combination with aspirin in the secondary prevention of myocardial infarction. A critical review of these trials indicates a range of evidence from no difference to a favorable trend that antiplatelet drugs may serve as anti-thrombotic agents in man. To date, a definitive answer concerning the therapeutic effects of these drugs in the secondary prevention of coronary heart disease is not available.

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Effects of a Monoclonal Antibody to Glycoprotein IIb/IIIa (P256) and of Enzymically Derived Fragments of P256 on Human Platelets

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648166 ◽

1991 ◽

Vol 65 (04) ◽

pp. 432-437 ◽

Cited By ~ 9

Author(s):

A W J Stuttle ◽

M J Powling ◽

J M Ritter ◽

R M Hardisty

Keyword(s):

Monoclonal Antibody ◽

Flow Cytometry ◽

Platelet Aggregation ◽

Calcium Ions ◽

Human Platelet ◽

Human Platelets ◽

In Vivo Detection ◽

Fibrinogen Binding ◽

Specific Antagonist

SummaryThe anti-platelet monoclonal antibody P256 is currently undergoing development for in vivo detection of thrombus. We have examined the actions of P256 and two fragments on human platelet function. P256, and its divalent fragment, caused aggregation at concentrations of 10−9−3 × 10−8 M. A monovalent fragment of P256 did not cause aggregation at concentrations up to 10−7 M. P256–induced platelet aggregation was dependent upon extracellular calcium ions as assessed by quin2 fluorescence. Indomethacin partially inhibited platelet aggregation and completely inhibited intracellular calcium mobilisation. Apyrase caused partial inhibition of aggregation. Aggregation induced by the divalent fragment was dependent upon fibrinogen and was inhibited by prostacyclin. Aggregation induced by the whole antibody was only partially dependent upon fibrinogen, but was also inhibited by prostacyclin. P256 whole antibody was shown, by flow cytometry, to induce fibrinogen binding to indomethacin treated platelets. Monovalent P256 was shown to be a specific antagonist for aggregation induced by the divalent forms. In–111–labelled monovalent fragment bound to gel-filtered platelets in a saturable and displaceable manner. Monovalent P256 represents a safer form for in vivo applications

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