High-contrast detection of target organisms in highly autofluorescent backgrounds using time-resolved techniques

Author(s):  
J.A. Piper ◽  
R.E. Connally ◽  
D.Y. Jin
Nano Today ◽  
2021 ◽  
Vol 40 ◽  
pp. 101264
Author(s):  
Gaoju Pang ◽  
Yingying Zhang ◽  
Xiaoyong Wang ◽  
Huizhuo Pan ◽  
Xinyu Zhang ◽  
...  
Keyword(s):  

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Richard B. Banati ◽  
Paul Wilcox ◽  
Ran Xu ◽  
Grace Yin ◽  
Emily Si ◽  
...  

An amendment to this paper has been published and can be accessed via a link at the top of the paper.


2020 ◽  
Vol 636 ◽  
pp. A72
Author(s):  
Frantz Martinache ◽  
Alban Ceau ◽  
Romain Laugier ◽  
Jens Kammerer ◽  
Mamadou N’Diaye ◽  
...  

Context. Kernel phase is a data analysis method based on a generalization of the notion of closure phase, which was invented in the context of interferometry, but it applies to well corrected diffraction dominated images produced by an arbitrary aperture. The linear model upon which it relies theoretically leads to the formation of observable quantities robust against residual aberrations. Aims. In practice, the detection limits that have been reported thus far seem to be dominated by systematic errors induced by calibration biases that were not sufficiently filtered out by the kernel projection operator. This paper focuses on the impact the initial modeling of the aperture has on these errors and introduces a strategy to mitigate them, using a more accurate aperture transmission model. Methods. The paper first uses idealized monochromatic simulations of a nontrivial aperture to illustrate the impact modeling choices have on calibration errors. It then applies the outlined prescription to two distinct data sets of images whose analysis has previously been published. Results. The use of a transmission model to describe the aperture results is a significant improvement over the previous type of analysis. The thus reprocessed data sets generally lead to more accurate results, which are less affected by systematic errors. Conclusions. As kernel-phase observing programs are becoming more ambitious, accuracy in the aperture description is becoming paramount to avoid situations where contrast detection limits are dominated by systematic errors. The prescriptions outlined in this paper will benefit from any attempt at exploiting kernel phase for high-contrast detection.


RSC Advances ◽  
2015 ◽  
Vol 5 (86) ◽  
pp. 70282-70286 ◽  
Author(s):  
Zhiyu Liao ◽  
Manuel Tropiano ◽  
Stephen Faulkner ◽  
Tom Vosch ◽  
Thomas Just Sørensen

Time-resolved NIR imaging of lanthanide coated silica particles using Photon Arrival Time Imaging allows fast acquisition of high contrast images based on the probe luminescence lifetime.


2018 ◽  
Vol 6 (21) ◽  
pp. 1800582 ◽  
Author(s):  
Jeeyoon Jeong ◽  
Hyeong Seok Yun ◽  
Dasom Kim ◽  
Kang Sup Lee ◽  
Han-Kyu Choi ◽  
...  

2019 ◽  
Vol 158 (1) ◽  
pp. 52
Author(s):  
Yifan Zhou ◽  
Dániel Apai ◽  
Ben W. P. Lew ◽  
Glenn Schneider ◽  
Elena Manjavacas ◽  
...  

2019 ◽  
Vol 157 (3) ◽  
pp. 128 ◽  
Author(s):  
Yifan Zhou ◽  
Dániel Apai ◽  
Ben W. P. Lew ◽  
Glenn Schneider ◽  
Elena Manjavacas ◽  
...  

2018 ◽  
Author(s):  
Wenwen Jing ◽  
Yan Wang ◽  
Yunze Yang ◽  
Yi Wang ◽  
Guangzhong Ma ◽  
...  

AbstractTimely diagnosis of acute diseases improves treatment outcomes and saves lives, but it requires fast and precision quantification of biomarkers. Here we report a time-resolved digital immunoassay based on plasmonic imaging of binding of single nanoparticles to biomarkers captured on a sensor surface. The real-time and high contrast of plasmonic imaging lead to fast and precise counting of the individual biomarkers over a wide dynamic range. We demonstrated the detection principle, evaluated the performance of the method using procalcitonin (PCT) as an example, and achieved a limit of detection of ~ 3 pg/mL, dynamic range of 4-12500 pg/mL, for a total detection time of ~ 25 mins.


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