Combined effect of proteins and AgNPs on the adhesion of yeast Candida albicans on solid silica surfaces

Introduction: Due to the emergence and spread of infections caused by Candida albicans especially among immunocompromised patients and increase in resistance by this group of fungi to commonly used antifungal agents, this study was undertaken to investigate the possible synergy between Garcinia kola seed and Vernonia amygdalina leaves extracts against organism. Methods: Ethanol extraction of Vernonia amygdalina leaves and Garcinia kola seeds were carried out and extract evaluated for anti-candida activities. The combined anti-candida activities of the extracts were also assessed using Checkerboard assay. Results: Extract of G. kola seed showed minimal anti-candida activity against the C. albicans with an inhibition zone of 5 mm and minimum inhibitory concentration of 50 mg/mL. The combined effect of both extract was demonstrated to be synergistic at most combination ratios tested. Furthermore, the combined effect was observed to have outweighed their individual effect, inhibiting the test isolates at concentration far less than the MICs of each of the extracts. The fractional inhibitory concentration index of the combined extracts at their various combination ratios against C. albicans ranged between 0.14 – 0.45 mg/ml indicating a synergistic effect by the various combinations against the organism. Conclusion: The combined activities by the two extracts showed marked improvement in anti-candida activities compared with their individual activities. The results support harnessing the individual bioactive constituents of the extracts and combining them for the development of potential antifungal agents.

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Combined Effect of Crude Leaf Extracts of Selected Medicinal Plants against Selected Enteric Bacterial Pathogens and Candida albicans

Journal of Antimicrobial Agents ◽

10.4172/2472-1212.1000110 ◽

2016 ◽

Vol 02 (01) ◽

Cited By ~ 1

Author(s):

Rachuonyo HO

Keyword(s):

Candida Albicans ◽

Medicinal Plants ◽

Bacterial Pathogens ◽

Combined Effect ◽

Leaf Extracts

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Synergistic Antifungal Activities of Thymol Analogues with Propolis

Natural Product Communications ◽

10.1177/1934578x0800300236 ◽

2008 ◽

Vol 3 (2) ◽

pp. 1934578X0800300 ◽

Cited By ~ 3

Author(s):

Chi-Pien Chen ◽

Ai-Yu Shen

Keyword(s):

Saccharomyces Cerevisiae ◽

Candida Albicans ◽

Antifungal Activity ◽

Inhibitory Activity ◽

Combined Effect ◽

Human Diseases ◽

Dilution Method ◽

Agar Dilution Method ◽

Antifungal Activities ◽

Agar Dilution

In this study, an attempt was made to elucidate the combined effect of thymol analogues with propolis on fungi that are involved in human diseases. Antifungal activity of thymol analogues was examined by the agar dilution method, using antibiotic medium-3-enriched with 0.2% dextrose. The activity of thymol analogues against Penicillium rastrickii appeared to be more marked than against Candida albicans and Saccharomyces cerevisiae. Various thymol analogues and propolis, when tested alone, did not exhibit significant inhibitory activity. However, in the presence of sub-inhibitory concentrations of propolis, the relative antifungal potency of the thymol analogues was greatly enhanced. Natural thymol was found to be superior to the synthetic thymol analogues. In particular, the MIC of thymol was considerably reduced (191-fold) against Candida albicans by combination with propolis. This study highlights the potential of the combination of either thymol or synthetic thymol analogues with propolis to inhibit fungi.

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