In Situ Visual Investigation of CO2 Bubbles Clogging Phenomena in ¿DMFC Anode Micro Flow Field

2006 ◽  
Author(s):  
Junsheng Liang ◽  
Chong Liu ◽  
Li Chen ◽  
Liding Wang
Keyword(s):  
Flow Field ◽  
Micro Flow

scholarly journals In situ measurement of micro flow rate using near infrared absorption method

2018 ◽  
Vol 26 (13) ◽  
pp. 17078 ◽  
Author(s):  
Seok Hwan Lee ◽  
Joohyun Lee ◽  
Sejong Chun ◽  
Woong Kang
Keyword(s):  
Flow Rate ◽  
Infrared Absorption ◽  
Near Infrared ◽  
In Situ Measurement ◽  
Absorption Method ◽  
Micro Flow

scholarly journals Inducing protein aggregation by extensional flow

2017 ◽  
Vol 114 (18) ◽  
pp. 4673-4678 ◽  
Author(s):  
John Dobson ◽  
Amit Kumar ◽  
Leon F. Willis ◽  
Roman Tuma ◽  
Daniel R. Higazi ◽  
...  
Keyword(s):  
Flow Field ◽  
Protein Aggregation ◽  
Large Scale ◽  
Extensional Flow ◽  
Cysteine Residue ◽  
Hydrodynamic Flow ◽  
Extrinsic Factors ◽  
Aggregation Propensity ◽  
Industrial Manufacture

Relative to other extrinsic factors, the effects of hydrodynamic flow fields on protein stability and conformation remain poorly understood. Flow-induced protein remodeling and/or aggregation is observed both in Nature and during the large-scale industrial manufacture of proteins. Despite its ubiquity, the relationships between the type and magnitude of hydrodynamic flow, a protein’s structure and stability, and the resultant aggregation propensity are unclear. Here, we assess the effects of a defined and quantified flow field dominated by extensional flow on the aggregation of BSA, β2-microglobulin (β2m), granulocyte colony stimulating factor (G-CSF), and three monoclonal antibodies (mAbs). We show that the device induces protein aggregation after exposure to an extensional flow field for 0.36–1.8 ms, at concentrations as low as 0.5 mg mL−1. In addition, we reveal that the extent of aggregation depends on the applied strain rate and the concentration, structural scaffold, and sequence of the protein. Finally we demonstrate the in situ labeling of a buried cysteine residue in BSA during extensional stress. Together, these data indicate that an extensional flow readily unfolds thermodynamically and kinetically stable proteins, exposing previously sequestered sequences whose aggregation propensity determines the probability and extent of aggregation.

In situ determination of the equivalence ratio in a methane/air flow field by femtosecond filament excitation

Laser Physics ◽  
2020 ◽  
Vol 30 (3) ◽  
pp. 035402
Author(s):  
Hongwei Zang ◽  
Danwen Yao ◽  
Siqi Wang ◽  
Yao Fu ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Flow Field ◽  
Equivalence Ratio ◽  
Air Flow

Numerical Simulation of Micro Flow Field on Biomimetic Sharkskin Micro-Grooved Surface

2014 ◽  
Vol 884-885 ◽  
pp. 378-381 ◽  
Author(s):  
Yue Hao Luo ◽  
Yu Fei Liu
Keyword(s):  
Numerical Simulation ◽  
Flow Field ◽  
Drag Reduction ◽  
Reduction Mechanism ◽  
Urgent Problem ◽  
Grooved Surface ◽  
Micro Flow ◽  
Important Significance

It is well-known that sharkskin surface has the effect of inhibiting the occurrence of turbulence and reducing the wall resistance, however, the drag reduction mechanism has developed into an urgent problem to be resolved now. According to the actual circumstance, for purpose of obtaining the best drag-reducing efficiency, the biomimetic sharkskin micro-grooved surface is designed according to the relevant literatures and research achievements, and numerical simulation of the micro flow field on the biomimetic sharkskin surface is carried out comprehensivley, which has the important significance to explain the drag reduction mechanism.

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