Time of Flight Micro Flow Sensor for Aqueous Fluids Using In-Situ Produced Tracer

2001 ◽  
pp. 1416-1419 ◽  
Author(s):  
Jian Wu ◽  
Willy Sansen
2020 ◽  
Author(s):  
Feifei Jia ◽  
Jie Wang ◽  
Yanyan Zhang ◽  
Qun Luo ◽  
Luyu Qi ◽  
...  

<p></p><p><i>In situ</i> visualization of proteins of interest at single cell level is attractive in cell biology, molecular biology and biomedicine, which usually involves photon, electron or X-ray based imaging methods. Herein, we report an optics-free strategy that images a specific protein in single cells by time of flight-secondary ion mass spectrometry (ToF-SIMS) following genetic incorporation of fluorine-containing unnatural amino acids as a chemical tag into the protein via genetic code expansion technique. The method was developed and validated by imaging GFP in E. coli and human HeLa cancer cells, and then utilized to visualize the distribution of chemotaxis protein CheA in E. coli cells and the interaction between high mobility group box 1 protein and cisplatin damaged DNA in HeLa cells. The present work highlights the power of ToF-SIMS imaging combined with genetically encoded chemical tags for <i>in situ </i>visualization of proteins of interest as well as the interactions between proteins and drugs or drug damaged DNA in single cells.</p><p></p>


2018 ◽  
Vol 26 (13) ◽  
pp. 17078 ◽  
Author(s):  
Seok Hwan Lee ◽  
Joohyun Lee ◽  
Sejong Chun ◽  
Woong Kang

Holzforschung ◽  
2017 ◽  
Vol 71 (9) ◽  
pp. 713-723 ◽  
Author(s):  
Francisco Arriaga ◽  
Daniel F. Llana ◽  
Miguel Esteban ◽  
Guillermo Íñiguez-González

Abstract The mechanical properties of timber can be estimated from wave propagation velocity by measuring wave time-of-flight (ToF). However, a time-lag complicates the measurements, which produces an apparent velocity dependency on length and this species and instrument dependent property is also influenced by knottiness. This research is dealing with time-lag determination by different sensor positioning in situ ToF measurements. ToF longitudinal measurements were conducted on 120 90 mm×140 mm specimens of the coniferous species radiata pine, Scots pine, laricio pine and maritime pine. The following commercially available acoustic devices were used: Sylvatest Duo, USLab, and Microsecond Timer. The sensors were arranged for the measurement types “end-to-end”, “on the same surface” and “on opposite surfaces”. ToF data were obtained from the full-length (4 m) specimens and then from the same specimens shortened to 3 m, 2 m and 1 m in length. The in situ procedures of ToF are applicable for a reliable length determination independently from the time-lag (tL) and velocity. The differences observed by end-to-end measurements, with respect to velocity, are below 4.4%. A velocity correction factor can be deduced for each instrument, which is independent of species.


2015 ◽  
Vol 11 (A29A) ◽  
pp. 305-306
Author(s):  
Marko Förstel ◽  
Ralf I. Kaiser

AbstractExploiting reflectron time of flight mass spectrometry coupled with single photon ionization of the subliming molecules (PI-ReTOF-MS) during the temperature programmed desorption (TPD) and combining these data with on line and in situ infrared spectroscopy (FTIR), a versatile experimental approach has been established to elucidate the formation pathways of complex organic molecules in interstellar analog ices upon interaction with ionizing radiation at astrophysically relevant temperatures as low as 5 K.


2014 ◽  
Vol 258 ◽  
pp. 356-364 ◽  
Author(s):  
I.A. Bobrikov ◽  
A.M. Balagurov ◽  
Chih-Wei Hu ◽  
Chih-Hao Lee ◽  
Tsan-Yao Chen ◽  
...  

2010 ◽  
Vol 24 (1) ◽  
pp. 439-445 ◽  
Author(s):  
M. Teresa Baeza-Romero ◽  
Jacqueline M. Wilson ◽  
Emma M. Fitzpatrick ◽  
Jenny M. Jones ◽  
Alan Williams

Sign in / Sign up

Export Citation Format

Share Document