Reciprocal plant‐mediated antagonism between a legume plant virus and soil rhizobia

2021 ◽  
Author(s):  
Saumik Basu ◽  
Robert E. Clark ◽  
Robert Blundell ◽  
Clare L. Casteel ◽  
Akaisha M. Charlton ◽  
...  
Keyword(s):  
Plant Virus ◽  
Legume Plant

Search mode for rapid detection of virus particles

1991 ◽  
Vol 49 ◽  
pp. 286-287
Author(s):  
O. E. Bradfute
Keyword(s):  
Electron Microscopy ◽  
Rapid Detection ◽  
Plant Virus ◽  
Low Dose ◽  
Search Mode ◽  
Virus Diseases ◽  
Virus Particles ◽  
Focus Image ◽  
Time Required ◽  
Rapid Switching

Electron microscopy is frequently used in preliminary diagnosis of plant virus diseases by surveying negatively stained preparations of crude extracts of leaf samples. A major limitation of this method is the time required to survey grids when the concentration of virus particles (VPs) is low. A rapid survey of grids for VPs is reported here; the method employs a low magnification, out-of-focus Search Mode similar to that used for low dose electron microscopy of radiation sensitive specimens. A higher magnification, in-focus Confirm Mode is used to photograph or confirm the detection of VPs. Setting up the Search Mode by obtaining an out-of-focus image of the specimen in diffraction (K. H. Downing and W. Chiu, private communications) and pre-aligning the image in Search Mode with the image in Confirm Mode facilitates rapid switching between Modes.

ALLELOPATHIC EFFECTS OF PARTHENIUM HYSTEROPHORUS ON GERMINATION OF SORGHUM SEEDS

2018 ◽  
Vol 6 (26) ◽  
Author(s):  
Jitendra Rajpoot
Keyword(s):  
Biological System ◽  
Growth And Development ◽  
Plant Virus ◽  
Evolutionary Origin ◽  
Plant Interactions ◽  
Biological Organization ◽  
Plant Soil ◽  
System A ◽  
Allelopathic Effects ◽  
Plant Plant

International Allelopathy Society has redefined Allelopathy as any process involving secondary metabolities produced by plants, algae, bacteria, fungi and viruses that influences the growth and development of agricultural and biological system; a study of the functions of secondary metabolities, their significance in biological organization, their evolutionary origin and elucidation of the mechanisms involving plant-plant, plant-microorganisms, plant-virus, plant-insect, plant-soil-plant interactions.

Plant Virus That Infects Honeybees May Harm Colonies

2014 ◽  
Vol 9 (4) ◽  
pp. 142-142
Author(s):  
Marcia Stone
Keyword(s):  
Plant Virus

scholarly journals Application of Oxford Nanopore Technology to Plant Virus Detection

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1424
Author(s):  
Lia W. Liefting ◽  
David W. Waite ◽  
Jeremy R. Thompson
Keyword(s):  
Plant Virus ◽  
High Throughput Sequencing ◽  
Virus Detection ◽  
Diagnostic Methods ◽  
Plant Virus Detection ◽  
Sequencing Technologies ◽  
Oxford Nanopore ◽  
Virus Diagnostics ◽  
Post Entry ◽  
Read Accuracy

The adoption of Oxford Nanopore Technologies (ONT) sequencing as a tool in plant virology has been relatively slow despite its promise in more recent years to yield large quantities of long nucleotide sequences in real time without the need for prior amplification. The portability of the MinION and Flongle platforms combined with lowering costs and continued improvements in read accuracy make ONT an attractive method for both low- and high-scale virus diagnostics. Here, we provide a detailed step-by-step protocol using the ONT Flongle platform that we have developed for the routine application on a range of symptomatic post-entry quarantine and domestic surveillance plant samples. The aim of this methods paper is to highlight ONT’s feasibility as a valuable component to the diagnostician’s toolkit and to hopefully stimulate other laboratories towards the eventual goal of integrating high-throughput sequencing technologies as validated plant virus diagnostic methods in their own right.

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