Co-expression of the proteinase inhibitors oryzacystatin I and oryzacystatin II in transgenic potato alters Colorado potato beetle larval development

After more than 10 years of research, Monsanto scientists have developed improved seed potatoes that are protected from serious pests, including insects and disease. The first commercial products resulting from this effort were NewLeaf ® potatoes derived from `Russet Burbank' and `Atlantic' parents. The NewLeaf® product was commercialized in 1995 and contains a gene from Bacillus thuringiensis (variety tenebrionis) (B.t.t.). for the production of the Cry3A protein. Potatoes expressing this gene are completely protected from the Colorado potato beetle (CPB) and need no additional chemical protection for this insect pest. The U.S. Food and Drug Administration (FDA), U.S. Dept. of Agriculture (USDA), and U.S. Environmental Protection Agency (EPA) have all determined that these potatoes are the same in safety and nutritional composition as any other `Russet Burbank' and `Atlantic' potatoes. These potatoes have also been approved by Health Canada, Agri-Food Canada and Agriculture Canada and by Japan and Mexico for food use. Commercial growers across North America have experienced outstanding performance while growing NewLeaf® potatoes 3 years in a row. This level of performance is the result of stable, nonsignificant differences in expression of the Cry3A gene. The stable performance, also, is a result of an effective insect resistance management program based on maintaining CPB refuges near NewLeaf ® fields, reducing CPB populations, and monitoring for CPB surviving exposure to NewLeaf® potatoes. In 1998 NewLeaf Y®), conferring resistance to both CPB and potato virus Y, and NewLeaf Plus®, conferring resistance to CPB and potato leafroll virus will be commercially released.

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Development of Bt-cry5 Insect-resistant Potato Lines 'Spunta-G2' and 'Spunta-G3'

HortScience ◽

10.21273/hortsci.37.7.1103 ◽

2002 ◽

Vol 37 (7) ◽

pp. 1103-1107 ◽

Cited By ~ 22

Author(s):

D.S. Douches ◽

W. Li ◽

K. Zarka ◽

J. Coombs ◽

W. Pett ◽

...

Keyword(s):

Potato Tuber ◽

Colorado Potato Beetle ◽

Insect Pest ◽

Transgenic Potato ◽

Potato Production ◽

The United States ◽

Field Trials ◽

Potato Tuber Moth ◽

Detached Leaf ◽

Potato Beetle

The potato tuber moth (Phthorimaea operculella Zeller) is the primary insect pest of cultivated potato (Solanum tuberosum L.) in tropical and subtropical regions, causing both foliar and tuber damage. In contrast, the Colorado potato beetle (Leptinotarsa decemlineata Say) is the most important insect pest in the northern potato production latitudes. The codon-modified Bacillus thuringiensis Bt-cry5 gene (revised nomenclature cry1IaI), specifically toxic to Lepidoptera and Coleoptera, was transformed into cultivar Spunta using an Agrobacterium vector to provide resistance to both potato tuber moth and Colorado potato beetle. The Bt-cry5 gene was placed downstream from the constitutive CaMV35S promoter. Two transgenic 'Spunta' clones, G2 and G3, produced high levels of mortality in first instars of potato tuber moth in detached-leaf bioassays (80% to 83% mortality), laboratory tuber tests (100% mortality), and field trials in Egypt (99% to 100% undamaged tubers). Reduced feeding by Colorado potato beetle first instars was also observed in detached-leaf bioassays (80% to 90% reduction). Field trials in the United States demonstrated that the horticultural performance of the two transgenic lines was comparable to 'Spunta'. These Bt-cry5 transgenic potato plants with high potato tuber moth resistance have value in integrated pest management programs.

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Transgenic potato plants expressing cry3A gene confer resistance to Colorado potato beetle

Comptes Rendus Biologies ◽

10.1016/j.crvi.2015.04.005 ◽

2015 ◽

Vol 338 (7) ◽

pp. 443-450 ◽

Cited By ~ 8

Author(s):

Xiaoxiao Mi ◽

Xiangzhuo Ji ◽

Jiangwei Yang ◽

Lina Liang ◽

Huaijun Si ◽

...

Keyword(s):

Colorado Potato Beetle ◽

Transgenic Potato ◽

Potato Plants ◽

Potato Beetle ◽

Transgenic Potato Plants

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MATING ABILITY OF COLORADO POTATO BEETLE (COLEOPTERA: CHRYSOMELIDAE) MALES FED TRANSGENIC POTATO (SOLANACEAE) FOLIAGE

The Canadian Entomologist ◽

10.4039/ent131539-4 ◽

1999 ◽

Vol 131 (4) ◽

pp. 539-540 ◽

Cited By ~ 1

Author(s):

Andrei V. Alyokhin ◽

David N. Ferro

Keyword(s):

Colorado Potato Beetle ◽

Leptinotarsa Decemlineata ◽

Selection Pressure ◽

Transgenic Potato ◽

Reproductive Activity ◽

Transgenic Crop ◽

Development Of Resistance ◽

Potato Beetle ◽

Important Pest ◽

Transgenic Potatoes

Colorado potato beetle, Leptinotarsa decemlineata (Say), is an important pest of potatoes Solanum tuberosum L. worldwide. To control this insect, commercial growers in North America may increasingly rely on planting transgenic potatoes that express the Bacillus thuringiensis (Berliner) subsp. tenebrionis Cry3A toxin in their foliage (Whalon and Ferro 1998). Because persistence and distribution of the toxin in these plants are maximized (Perlak et al. 1993), there is strong selection pressure towards development of resistance to the Cry3A toxin in beetle populations (Ferro 1993). One strategy proposed to delay development of resistance is to provide spatial refugia of nontransgenic potatoes to support populations of susceptible individuals sufficient to curtail matings between resistant individuals (Whalon and Ferro 1998). Therefore, it is important that susceptible beetles moving into the transgenic crop from refugia can successfully mate with local resistant beetles. Wierenga et al. (1996) reported that adult beetles that fed continuously on transgenic potatoes since eclosion from pupae did not display any reproductive activity, but that successful reproduction was eventually observed if beetles fed on nontransgenic foliage later. Under field conditions, however, it is more likely that susceptible beetles emerging from pupae in refugia will feed on nontransgenic potatoes before, not after, moving onto transgenic plants. We have recently discovered that under such circumstances female beetles remain reproductive, but with significantly reduced fecundity relative to control females continuously fed on nontransgenic foliage (Alyokhin and Ferro 1999). No such data are currently available for male beetles.

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Constitutive Expression of cry3A Gene in Transgenic Potato Plants for Resistance to Colorado Potato Beetle (CPB)

Potato Research ◽

10.1007/s11540-021-09500-5 ◽

2021 ◽

Author(s):

Hamed Salehian ◽

Hassan Rahnama ◽

Sara Dezhsetan ◽

Saeid Babaei

Keyword(s):

Colorado Potato Beetle ◽

Constitutive Expression ◽

Transgenic Potato ◽

Potato Plants ◽

Potato Beetle ◽

Transgenic Potato Plants

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Field Performance of Transgenic Potato, with Resistance to Colorado Potato Beetle and Viruses

HortScience ◽

10.21273/hortsci.37.2.275 ◽

2002 ◽

Vol 37 (2) ◽

pp. 275-276 ◽

Cited By ~ 12

Author(s):

David R. Duncan ◽

David Hammond ◽

Jim Zalewski ◽

John Cudnohufsky ◽

Wojciech Kaniewski ◽

...

Keyword(s):

Colorado Potato Beetle ◽

Field Performance ◽

Transgenic Potato ◽

Potato Beetle

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Examining the molecular interaction between potato (Solanum tuberosum) and Colorado potato beetle Leptinotarsa decemlineata

Botany ◽

10.1139/b08-074 ◽

2008 ◽

Vol 86 (9) ◽

pp. 1080-1091 ◽

Cited By ~ 9

Author(s):

Susan D. Lawrence ◽

Nicole G. Novak ◽

Chelsea J.-T. Ju ◽

Janice E.K. Cooke

Keyword(s):

Colorado Potato Beetle ◽

Molecular Interaction ◽

Expressed Sequence Tag ◽

Proteinase Inhibitors ◽

Differentially Expressed ◽

Defensive Chemistry ◽

Potato Beetle ◽

Tropinone Reductase ◽

Continuous Feeding ◽

Solanaceous Plants

Colorado potato beetle (CPB) is a devastating herbivorous pest of solanaceous plants. Despite the economic impact, little is known about the molecular interaction of CPB with these plants. Using an 11 421 expressed sequence tag (EST) potato microarray, we identified 320 genes differentially expressed in potato leaves in response to CPB herbivory. Amongst these were genes putatively encoding proteinase inhibitors along with enzymes of terpenoid, alkaloid, and phenylpropanoid biosynthetic pathways, suggesting the defensive chemistries that constitute potato’s defense against CPB herbivory. Several genes, such as those encoding proteinase inhibitors, represent mechanisms implicated in other plant–herbivory interactions, and could correspond with general defensive chemistry strategies. In other cases, products of the differentially expressed genes may represent taxa-specific defensive chemistry. For example, the presumed alkaloid products of a putative tropinone reductase I are specific to a subset of the Solanaceae. Two herbivory-induced genes, not specific to potato, are implicated in the synthesis of volatiles known to attract CPB predators. Comparison of continuous herbivore attack versus recovery from CPB attack indicates that fewer genes involved in defensive chemistry are induced after continuous feeding than after feeding and recovery, suggesting the plant’s ability to mount a full defense response is enhanced under light versus heavy attack.

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