Nitrous oxide emissions associated with ammonia‐oxidizing bacteria abundance in fields of switchgrass with and without intercropped alfalfa

ABSTRACT A molecular analysis of betaproteobacterial ammonia oxidizers and a N2O isotopomer analysis were conducted to study the sources of N2O emissions during the cow manure composting process. Much NO2 −-N and NO3 −-N and the Nitrosomonas europaea-like amoA gene were detected at the surface, especially at the top of the composting pile, suggesting that these ammonia-oxidizing bacteria (AOB) significantly contribute to the nitrification which occurs at the surface layer of compost piles. However, the 15N site preference within the asymmetric N2O molecule (SP = δ15Nα − δ15Nβ, where 15Nα and 15Nβ represent the 15N/14N ratios at the center and end sites of the nitrogen atoms, respectively) indicated that the source of N2O emissions just after the compost was turned originated mainly from the denitrification process. Based on these results, the reduction of accumulated NO2 −-N or NO3 −-N after turning was identified as the main source of N2O emissions. The site preference and bulk δ15N results also indicate that the rate of N2O reduction was relatively low, and an increased value for the site preference indicates that the nitrification which occurred mainly in the surface layer of the pile partially contributed to N2O emissions between the turnings.

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Nitrous oxide emissions from wastewater treatment processes

Philosophical Transactions of the Royal Society B Biological Sciences ◽

10.1098/rstb.2011.0317 ◽

2012 ◽

Vol 367 (1593) ◽

pp. 1265-1277 ◽

Cited By ~ 241

Author(s):

Yingyu Law ◽

Liu Ye ◽

Yuting Pan ◽

Zhiguo Yuan

Keyword(s):

Nitrous Oxide ◽

Wastewater Treatment ◽

Wastewater Treatment Plants ◽

High Water ◽

Nitrous Oxide Emissions ◽

Ammonia Oxidizing Bacteria ◽

Plant Design ◽

Operational Conditions ◽

Nitrifier Denitrification ◽

Wastewater Treatment Systems

Nitrous oxide (N 2 O) emissions from wastewater treatment plants vary substantially between plants, ranging from negligible to substantial (a few per cent of the total nitrogen load), probably because of different designs and operational conditions. In general, plants that achieve high levels of nitrogen removal emit less N 2 O, indicating that no compromise is required between high water quality and lower N 2 O emissions. N 2 O emissions primarily occur in aerated zones/compartments/periods owing to active stripping, and ammonia-oxidizing bacteria, rather than heterotrophic denitrifiers, are the main contributors. However, the detailed mechanisms remain to be fully elucidated, despite strong evidence suggesting that both nitrifier denitrification and the chemical breakdown of intermediates of hydroxylamine oxidation are probably involved. With increased understanding of the fundamental reactions responsible for N 2 O production in wastewater treatment systems and the conditions that stimulate their occurrence, reduction of N 2 O emissions from wastewater treatment systems through improved plant design and operation will be achieved in the near future.

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Effects of the Fertilizer Added with DMPP on Soil Nitrous Oxide Emissions and Microbial Functional Diversity

Agriculture ◽

10.3390/agriculture11010012 ◽

2020 ◽

Vol 11 (1) ◽

pp. 12

Author(s):

Anna Tedeschi ◽

Anna De Marco ◽

Franca Polimeno ◽

Paul Di Tommasi ◽

Giuseppe Maglione ◽

...

Keyword(s):

Nitrous Oxide ◽

Microbial Biomass ◽

Microbial Biomass Carbon ◽

Potato Crop ◽

N2o Emission ◽

Nitrous Oxide Emissions ◽

Nitrification Inhibitors ◽

Ammonia Oxidizing Bacteria ◽

N2o Production ◽

The Impact

Agricultural sites contribute extensively to atmospheric emissions of climate-altering gases such as nitrous oxide. Several strategies have been considered to mitigate the impact of agriculture on climate, among these the utilization of fertilizers added with nitrification inhibitors such as DMPP (3,4-dimethylpyrazole phosphate) may represent a suitable solution. DMPP inhibits the growth and activity of ammonia-oxidizing microorganisms, particularly the ammonia-oxidizing bacteria, which are involved in N2O production. At present, little information is available on the effects of DMPP on the catabolic diversity of soil microbial community. In this study, the N2O emission by soil was performed by using the static chamber technique. The biological determinations of the microbial biomass carbon and the catabolic profile were assessed by measuring the substrate-induced respiration during the entire growing season of a potato crop under two nitrogen treatments: fertilization with and without DMPP. Our results did not show a clear mitigation of N2O emission by DMPP, even if a tendency to lower N2O fluxes in DMPP plots occurred when soil temperatures were lower than 20 °C. Conversely, DMPP deeply affected the microbial biomass and the catabolism of soil microorganisms, exerting a negative effect when it accumulated in excessive doses in the soil, limiting the growth and the capacity of soil microorganism communities to use different substrates.

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A regionally disaggregated inventory of nitrous oxide emissions from agricultural soils in Germany – a GIS-based empirical approach

Erdkunde ◽

10.3112/erdkunde.2014.02.04 ◽

2014 ◽

Vol 68 (2) ◽

pp. 125-144 ◽

Cited By ~ 9

Author(s):

Sebastian Brocks ◽

Hermann F. Jungkunst ◽

Georg Bareth

Keyword(s):

Nitrous Oxide ◽

Agricultural Soils ◽

Empirical Approach ◽

Nitrous Oxide Emissions

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Effects of Straw Recycling of Winter Covering Crop on Methane and Nitrous Oxide Emissions in Paddy Field

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2011.01666 ◽

2011 ◽

Vol 37 (9) ◽

pp. 1666-1675

Author(s):

Hai-Ming TANG ◽

Xiao-Ping XIAO ◽

Wen-Guang TANG ◽

Guang-Li YANG

Keyword(s):

Nitrous Oxide ◽

Paddy Field ◽

Nitrous Oxide Emissions

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Biochar and urease inhibitor mitigate NH3 and N2O emissions and improve wheat yield in a urea fertilized alkaline soil

Scientific Reports ◽

10.1038/s41598-021-96771-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Khadim Dawar ◽

Shah Fahad ◽

M. M. R. Jahangir ◽

Iqbal Munir ◽

Syed Sartaj Alam ◽

...

Keyword(s):

Nitrous Oxide ◽

Grain Yield ◽

Block Design ◽

N Uptake ◽

Nitrous Oxide Emissions ◽

N2o Emissions ◽

Urease Inhibitor ◽

Alkaline Soil ◽

Total N ◽

N Assimilation

AbstractIn this study, we explored the role of biochar (BC) and/or urease inhibitor (UI) in mitigating ammonia (NH3) and nitrous oxide (N2O) discharge from urea fertilized wheat cultivated fields in Pakistan (34.01°N, 71.71°E). The experiment included five treatments [control, urea (150 kg N ha−1), BC (10 Mg ha−1), urea + BC and urea + BC + UI (1 L ton−1)], which were all repeated four times and were carried out in a randomized complete block design. Urea supplementation along with BC and BC + UI reduced soil NH3 emissions by 27% and 69%, respectively, compared to sole urea application. Nitrous oxide emissions from urea fertilized plots were also reduced by 24% and 53% applying BC and BC + UI, respectively, compared to urea alone. Application of BC with urea improved the grain yield, shoot biomass, and total N uptake of wheat by 13%, 24%, and 12%, respectively, compared to urea alone. Moreover, UI further promoted biomass and grain yield, and N assimilation in wheat by 38%, 22% and 27%, respectively, over sole urea application. In conclusion, application of BC and/or UI can mitigate NH3 and N2O emissions from urea fertilized soil, improve N use efficiency (NUE) and overall crop productivity.

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