scholarly journals Bioinformatics Analyses Reveal Age-Specific Neuroimmune Modulation as a Target for Treatment of High Ethanol Drinking

2013 ◽  
Vol 38 (2) ◽  
pp. 428-437 ◽  
Author(s):  
Rajiv G. Agrawal ◽  
Julie A. Owen ◽  
Patricia S. Levin ◽  
Aveline Hewetson ◽  
Ari E. Berman ◽  
...  
Keyword(s):  
Bioinformatics Analyses ◽  
Ethanol Drinking ◽  
Neuroimmune Modulation ◽  
High Ethanol

Ethanol Palatability and Consumption by High Ethanol-Drinking Rats: Manipulation of the Opioid System With Naltrexone.

2004 ◽  
Vol 118 (5) ◽  
pp. 1089-1096 ◽  
Author(s):  
Daniel L. Coonfield ◽  
Stephen W. Kiefer ◽  
Frank M. Ferraro ◽  
John David Sinclair
Keyword(s):  
Opioid System ◽  
Ethanol Drinking ◽  
High Ethanol

scholarly journals Effective Reduction in High Ethanol Drinking by Semisynthetic Tetracycline Derivatives

2016 ◽  
Vol 40 (12) ◽  
pp. 2482-2490 ◽  
Author(s):  
Peter J. Syapin ◽  
Joseph M. Martinez ◽  
David C. Curtis ◽  
Patrick C. Marquardt ◽  
Clayton L. Allison ◽  
...  
Keyword(s):  
Ethanol Drinking ◽  
Effective Reduction ◽  
High Ethanol

Activation of Melanocortin-4 Receptor by a Synthetic Agonist Inhibits Ethanolinduced Neuroinflammation in Rats

2020 ◽  
Vol 25 (45) ◽  
pp. 4799-4805 ◽  
Author(s):  
Osvaldo Flores-Bastías ◽  
Gonzalo I. Gómez ◽  
Juan A. Orellana ◽  
Eduardo Karahanian
Keyword(s):  
Prefrontal Cortex ◽  
Alcohol Consumption ◽  
Ethanol Intake ◽  
Neuroinflammatory Response ◽  
Agonist Peptide ◽  
Melanocortin 4 Receptor ◽  
Tnf Α ◽  
Cord Injury ◽  
High Ethanol ◽  
The Brain

Background: High ethanol intake induces a neuroinflammatory response resulting in the subsequent maintenance of chronic alcohol consumption. The melanocortin system plays a pivotal role in the modulation of alcohol consumption. Interestingly, it has been shown that the activation of melanocortin-4 receptor (MC4R) in the brain decreases the neuroinflammatory response in models of brain damage other than alcohol consumption, such as LPS-induced neuroinflammation, cerebral ischemia, glutamate excitotoxicity, and spinal cord injury. Objectives: In this work, we aimed to study whether MC4R activation by a synthetic MC4R-agonist peptide prevents ethanol-induced neuroinflammation, and if alcohol consumption produces changes in MC4R expression in the hippocampus and hypothalamus. Methods: Ethanol-preferring Sprague Dawley rats were selected offering access to 20% ethanol on alternate days for 4 weeks (intermittent access protocol). After this time, animals were i.p. administered an MC4R agonist peptide in the last 2 days of the protocol. Then, the expression of the proinflammatory cytokines interleukin 6 (IL-6), interleukin 1-beta (IL-1β), and tumor necrosis factor-alpha (TNF-α) were measured in the hippocampus, hypothalamus and prefrontal cortex. It was also evaluated if ethanol intake produces alterations in the expression of MC4R in the hippocampus and the hypothalamus. Results: Alcohol consumption increased the expression of MC4R in the hippocampus and the hypothalamus. The administration of the MC4R agonist reduced IL-6, IL-1β and TNF-α levels in hippocampus, hypothalamus and prefrontal cortex, to those observed in control rats that did not drink alcohol. Conclusion: High ethanol consumption produces an increase in the expression of MC4R in the hippocampus and hypothalamus. The administration of a synthetic MC4R-agonist peptide prevents neuroinflammation induced by alcohol consumption in the hippocampus, hypothalamus, and prefrontal cortex. These results could explain the effect of α-MSH and other synthetic MC4R agonists in decreasing alcohol intake through the reduction of the ethanol-induced inflammatory response in the brain.

Systematic Annotation and Bioinformatics Analyses of Large-Scale Oryza sativa Proteome

2011 ◽  
Vol 12 (7) ◽  
pp. 621-630 ◽  
Author(s):  
Lili Liu ◽  
Lin Bai ◽  
Cong Luo ◽  
Donglin Huang ◽  
Ming Chen
Keyword(s):  
Oryza Sativa ◽  
Large Scale ◽  
Bioinformatics Analyses

The contribution of medium spiny neuron subtypes in the nucleus accumbens core to compulsive-like ethanol drinking

2021 ◽  
Vol 187 ◽  
pp. 108497 ◽  
Author(s):  
Elizabeth A. Sneddon ◽  
Kristen M. Schuh ◽  
John W. Frankel ◽  
Anna K. Radke
Keyword(s):  
Nucleus Accumbens ◽  
Medium Spiny Neuron ◽  
Nucleus Accumbens Core ◽  
Ethanol Drinking ◽  
Accumbens Core

scholarly journals Unsuspected somatic mosaicism for FBN1 gene contributes to Marfan syndrome

2021 ◽  
Author(s):  
Pauline Arnaud ◽  
Hélène Morel ◽  
Olivier Milleron ◽  
Laurent Gouya ◽  
Christine Francannet ◽  
...  
Keyword(s):  
Marfan Syndrome ◽  
Somatic Mosaicism ◽  
Variant Calling ◽  
Copy Number Variations ◽  
Pathogenic Variant ◽  
Single Nucleotide Variants ◽  
Bioinformatics Analyses ◽  
Single Nucleotide ◽  
Fbn1 Gene ◽  
Pathogenic Variants

Abstract Purpose Individuals with mosaic pathogenic variants in the FBN1 gene are mainly described in the course of familial screening. In the literature, almost all these mosaic individuals are asymptomatic. In this study, we report the experience of our team on more than 5,000 Marfan syndrome (MFS) probands. Methods Next-generation sequencing (NGS) capture technology allowed us to identify five cases of MFS probands who harbored a mosaic pathogenic variant in the FBN1 gene. Results These five sporadic mosaic probands displayed classical features usually seen in Marfan syndrome. Combined with the results of the literature, these rare findings concerned both single-nucleotide variants and copy-number variations. Conclusion This underestimated finding should not be overlooked in the molecular diagnosis of MFS patients and warrants an adaptation of the parameters used in bioinformatics analyses. The five present cases of symptomatic MFS probands harboring a mosaic FBN1 pathogenic variant reinforce the fact that apparently asymptomatic mosaic parents should have a complete clinical examination and a regular cardiovascular follow-up. We advise that individuals with a typical MFS for whom no single-nucleotide pathogenic variant or exon deletion/duplication was identified should be tested by NGS capture panel with an adapted variant calling analysis.

scholarly journals A novel homeostatic loop of sorcin drives paclitaxel-resistance and malignant progression via Smad4/ZEB1/miR-142-5p in human ovarian cancer

Oncogene ◽  
2021 ◽  
Author(s):  
Jinguo Zhang ◽  
Wencai Guan ◽  
Xiaolin Xu ◽  
Fanchen Wang ◽  
Xin Li ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Calcium Binding ◽  
Molecular Mechanisms ◽  
Epithelial Mesenchymal Transition ◽  
Malignant Progression ◽  
Bioinformatics Analyses ◽  
Aberrant Expression ◽  
Mesenchymal Transition ◽  
E Box

AbstractThe primary chemotherapy of ovarian cancer (OC) often acquires chemoresistance. Sorcin (SRI), a soluble resistance-related calcium-binding protein, has been reported to be an oncogenic protein in cancer. However, the molecular mechanisms of SRI regulation and the role and aberrant expression of SRI in chemoresistant OC remain unclear. Here, we identified SRI as a key driver of paclitaxel (PTX)-resistance and explored its regulatory mechanism. Using transcriptome profiles, qRT-PCR, proteomics, Western blot, immunohistochemistry, and bioinformatics analyses, we found that SRI was overexpressed in PTX-resistant OC cells and the overexpression of SRI was related to the poor prognosis of patients. SRI was a key molecule required for growth, migration, and PTX-resistance in vitro and in vivo and was involved in epithelial–mesenchymal transition (EMT) and stemness. Mechanistic studies showed that miR-142-5p directly bound to the 3ʹ-UTR of SRI to suppress its expression, whereas a transcription factor zinc-finger E-box binding homeobox 1 (ZEB1) inhibited the transcription of miR-142-5p by directly binding to the E-box fragment in the miR-142 promoter region. Furthermore, ZEB1 was negatively regulated by SRI which physically interacted with Smad4 to block its translocation from the cytosol to the nucleus. Taken together, our findings unveil a novel homeostatic loop of SRI that drives the PTX-resistance and malignant progression via Smad4/ZEB1/miR-142-5p in human OC. Targeting this SRI/Smad4/ZEB1/miR-142-5p loop may reverse the PTX-resistance.

Development of an ethanolic two-phase system (ETPS) based on polypropylene glycol 2000 + ethylene glycol + ethanol for separation of hydrophobic compounds

2021 ◽  
Author(s):  
Filipe Smith Buarque ◽  
Cleide Mara Faria Soares ◽  
Ranyere Lucena de Souza ◽  
Matheus Mendonça Pereira ◽  
Álvaro Silva Lima
Keyword(s):  
Ethylene Glycol ◽  
Phase System ◽  
Polypropylene Glycol ◽  
Two Phase ◽  
Hydrophobic Compounds ◽  
Ethanol Content ◽  
Coexisting Phases ◽  
Phase Water ◽  
High Ethanol

Two-phase water-free systems containing high ethanol content in the coexisting phases can selectively partition hydrophobic molecules from natural biomass.

scholarly journals ARL4C might serve as a prognostic factor and a novel therapeutic target for gastric cancer: bioinformatics analyses and biological experiments

2021 ◽  
Vol 25 (8) ◽  
pp. 4014-4027
Author(s):  
Ning Xie ◽  
Yunfan Bai ◽  
Lu Qiao ◽  
Yuru Bai ◽  
Jian Wu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Prognostic Factor ◽  
Therapeutic Target ◽  
Bioinformatics Analyses ◽  
Novel Therapeutic ◽  
Cancer Bioinformatics
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