Downregulation of polymeric immunoglobulin receptor and secretory IgA antibodies in eosinophilic upper airway diseases

Allergy ◽  
2013 ◽  
Vol 68 (12) ◽  
pp. 1589-1597 ◽  
Author(s):  
C. Hupin ◽  
P. Rombaux ◽  
H. Bowen ◽  
H. Gould ◽  
M. Lecocq ◽  
...  
Keyword(s):  
Upper Airway ◽  
Secretory Iga ◽  
Polymeric Immunoglobulin Receptor ◽  
Immunoglobulin Receptor ◽  
Airway Diseases ◽  
Iga Antibodies

scholarly journals TNF-α Mediated Secretory Component is Positively Regulated by PI3K/AKT/MTOR in CACO-2 Cells

2013 ◽  
Vol 32 (2) ◽  
pp. 116-120 ◽  
Author(s):  
Dongyan Liu ◽  
Si Wang ◽  
Teng Jiang ◽  
Peng Ding
Keyword(s):  
Signaling Pathway ◽  
Real Time Pcr ◽  
Secretory Component ◽  
Secretory Iga ◽  
Polymeric Immunoglobulin Receptor ◽  
Mtor Signaling Pathway ◽  
Quantitative Real Time Pcr ◽  
Immunoglobulin Receptor ◽  
Tnf Α ◽  
Polymeric Immunoglobulins

Summary Background: Secretory component (SC) is the extracellular component of the polymeric immunoglobulin receptor (pIgR) that functions as a receptor for externally secreted polymeric immunoglobulins such as secretory IgA. SC expression is regulated by a signaling pathway involving TNF-a. Methods: Caco-2 cells were cotreated with TNF-a plus either Wortmannin, which inhibits PI3K, or Rapamycin, which inhibits mTOR. The expression of SC and pIgR mRNA were assessed by immunocytochemistry, Western blotting, and Quantitative real-time PCR. Results: Wortmannin and Rapamycin decreased the fraction of cells expressing SC, as well as the total expression of SC protein and pIgR mRNA. Conclusions: TNF-a regulation of SC expression is mediated through a PI3K/AKT/mTOR signaling pathway in Caco-2 cells.

scholarly journals Expression of the polymeric immunoglobulin receptor and excretion of secretory IgA in the postischemic kidney

1999 ◽  
Vol 276 (5) ◽  
pp. F666-F673 ◽  
Author(s):  
James C. Rice ◽  
Jeff S. Spence ◽  
Judit Megyesi ◽  
Randall M. Goldblum ◽  
Robert L. Safirstein
Keyword(s):  
Mucosal Immune Response ◽  
Secretory Component ◽  
Secretory Iga ◽  
Polymeric Immunoglobulin Receptor ◽  
Thick Ascending Limb ◽  
Mrna Levels ◽  
Immunoglobulin Receptor ◽  
Medullary Thick Ascending Limb ◽  
Tract Infections

The humoral mucosal immune response of the kidney involves the transport of secretory IgA (S-IgA) through renal epithelial cells by the polymeric immunoglobulin receptor (pIgR). The pIgR is cleaved and released as free secretory component (FSC) or attached to IgA (S-IgA). We examined the effects of an ischemic model of acute renal failure (ARF) on the expression of pIgR and the secretion of FSC and S-IgA in the urine. Kidney pIgR mRNA levels decreased in ischemic animals by 55% at 4 h and by 85% at 72 h compared with controls. pIgR protein expression in the medullary thick ascending limb (TAL) decreased within 24 h and was nearly undetectable by 72 h. Urinary S-IgA and FSC concentrations decreased by 60% between days 3 and 6. pIgR mRNA and pIgR protein in the kidney returned to ∼90% of control levels and urinary FSC and S-IgA concentrations returned to ∼55% of control levels by day 7. We demonstrate that ischemic ARF decreases renal mucosal S-IgA transport in vivo and may contribute to the increased incidence of urinary tract infections.

A study on the correlation between upper airway diseases and urinary heavy metals concentration in household residents

2018 ◽  
Vol 17 (1) ◽  
pp. 78-84 ◽  
Author(s):  
Jihee Choi ◽  
◽  
Yujin Oh ◽  
Yunjae Kim ◽  
Munseob Ahn ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Upper Airway ◽  
Airway Diseases

Multidisciplinary Care for Severe or Uncontrolled Chronic Upper Airway Diseases

2021 ◽  
Vol 21 (4) ◽  
Author(s):  
Ioannis Vlastos ◽  
Joaquim Mullol ◽  
Valerie Hox ◽  
Maria Doulaptsi ◽  
Sven Seys ◽  
...  
Keyword(s):  
Upper Airway ◽  
Multidisciplinary Care ◽  
Airway Diseases

scholarly journals Antibody in Middle Ear Fluid of Children Originates Predominantly from Sera and Nasopharyngeal Secretions

2012 ◽  
Vol 19 (10) ◽  
pp. 1593-1596 ◽  
Author(s):  
Ravinder Kaur ◽  
Thomas Kim ◽  
Janet R. Casey ◽  
Michael E. Pichichero
Keyword(s):  
Middle Ear ◽  
Acute Otitis Media ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Two Dimensional Gel Electrophoresis ◽  
Middle Ear Fluid ◽  
Iga Antibodies ◽  
Iga Antibody ◽  
Entire Array

ABSTRACTThe human middle ear is devoid of any immunocompetent cells in normal mucosa. We sought to determine the source of antibody present in the middle ear of children. Total IgG, IgA, and secretory IgA antibodies were determined by enzyme-linked immunosorbent assay from the nasopharyngeal, middle ear, and serum samples of children with acute otitis media. The two-dimensional gel electrophoresis pattern of the entire array of IgA antibodies in the nasal wash (NW) and middle ear fluid (MEF) was compared from the MEF and NW samples using isoelectric focusing and Western blotting. The total IgG and IgA antibodies in the MEF and NW samples of 137 children were compared. The ratio of IgG to IgA in the MEF was significantly different (P< 0.008) compared to NW because IgA levels were higher and IgG levels lower in NW. The IgG/IgA ratio of MEF resembled serum consistent with transudation to the MEF. Small amounts of secretory IgA were detected in MEF but the electrophoresis patterns of the entire array of IgA antibodies in the MEF and NW were virtually identical in each child evaluated; thus, IgA in MEF derived predominantly from serum and the nasopharynx by reflux via the Eustachian tube. The IgG/IgA antibody levels in the MEF and the same composition of IgA antibody in the MEF and NW identifies the predominant source of antibody in the MEF as a transudate of serum combined with nasal secretions refluxed from the nasopharynx in children.

scholarly journals Rifaximin as a Potential Treatment for IgA Nephropathy in a Humanized Mice Model

2021 ◽  
Vol 11 (4) ◽  
pp. 309
Author(s):  
Vincenzo Di Leo ◽  
Patrick J. Gleeson ◽  
Fabio Sallustio ◽  
Carine Bounaix ◽  
Jennifer Da Silva ◽  
...  
Keyword(s):  
Iga Nephropathy ◽  
Serum Levels ◽  
Humanized Mice ◽  
Polymeric Immunoglobulin Receptor ◽  
Oral Antibiotic ◽  
Mice Model ◽  
Immunoglobulin Receptor ◽  
Tnf Α ◽  
Factor Α ◽  
Growth Of Bacteria

IgA Nephropathy (IgAN) is the most common glomerulonephritis worldwide, characterized by the mesangial deposition of abnormally glycosylated IgA1 (Gd-IgA). The production of Gd-IgA occurs in mucose-associated lymphoid tissue (MALT). The microbiota plays a role in MALT modulation. Rifaximin (NORMIX®), a non-absorbable oral antibiotic, induces positive modulation of the gut microbiota, favoring the growth of bacteria beneficial to the host. Here, we evaluate the effect of rifaximin on a humanized mice model of IgAN (α1KI-CD89Tg). Methods: The α1KI-CD89Tg mice were treated by the vehicle (olive oil) or rifaximin (NORMIX®). Serum levels of hIgA, hIgA1–sCD89, and mIgG–hIgA1 immune complexes were determined. Glomerular hIgA1 deposit and CD11b+ cells recruitment were revealed using confocal microscopy. Furthermore, the mRNA of the B-Cell Activating Factor (BAFF), polymeric immunoglobulin receptor (pIgR), and Tumor Necrosing Factor-α (TNF-α) in gut samples were detected by qPCR. Results: Rifaximin treatment decreased the urinary protein-to-creatinine ratio, serum levels of hIgA1–sCD89 and mIgG–hIgA1 complexes, hIgA1 glomerular deposition, and CD11b+ cell infiltration. Moreover, rifaximin treatment decreased significantly BAFF, pIgR, and TNF-α mRNA expression. Conclusions: Rifaximin decreased the IgAN symptoms observed in α1KI-CD89Tg mice, suggesting a possible role for it in the treatment of the disease.

Sign in / Sign up

Export Citation Format

Share Document