Interaction among extenders, cryoprotectants and Orvus Es Paste supplementation and freezing rate for sperm cryopreservation in the dromedary camel

The spotted halibut is species that has a high potential market value in Korea, but the supply of seed is unstable because of the limited milt production of males. The objective of this research was to explore different aspects, such as CPAs, diluents, dilution ratio, and freezing rates, to develop an optimal sperm cryopreservation. The parameters assessed were movable sperm ratio, sperm activity index, survival rate, and DNA damage. The CPAs tested in this research were propylene glycol, dimethyl sulfoxide (DMSO), methanol, ethylene glycol, and glycerol. Different diluents, including 300 mM sucrose, 300 mM glucose, Stain’s solution, and Ringer’s solution, were investigated. The previous experiment showed that the optimal CPA for cryopreservation was DMSO with a concentration of 15% with 300 mM as diluent. To determine the effect of the dilution ratio, sperm was diluted to 1:1, 1:2, 1:10, 1:100, and 1:1000 with 300 mM sucrose containing DMSO at a final concentration of 15%. Lastly, the optimal freezing rate of the sperm was evaluated with four different freezing rates (−1, −5, −10, and −20 °C/min). Post-thaw sperm motility was higher with a dilution ratio lower than 1:2, and the freezing rate was less than −5 °C/min. In conclusion, these findings represent the development of a cryopreservation protocol for spotted halibut.

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Effect of pre-freezing rate on porosity ratio and mechanical property of pig aorta

Frontiers in Bioscience ◽

10.2741/3945 ◽

2012 ◽

Vol 17 (1) ◽

pp. 575 ◽

Cited By ~ 2

Author(s):

Meng Yin

Keyword(s):

Mechanical Property ◽

Freezing Rate ◽

Porosity Ratio

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Evaluation of Hematological and Biochemical Changes in Dromedary Camel during the Different Stages of Lactation

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2020.21.320 ◽

2020 ◽

Vol 21 (3) ◽

pp. 121-124

Author(s):

Ahmed El-Sayed

Keyword(s):

Organic Phosphorus ◽

Hemoglobin Concentration ◽

Controlled Study ◽

Mean Corpuscular Hemoglobin ◽

Dromedary Camel ◽

Gamma Glutamyl Transferase ◽

Corpuscular Hemoglobin ◽

Stage Of Lactation ◽

Group 2 ◽

Glutamyl Transferase

Objective: To assess the potential hematobiochemical alterations in healthy dromedary camel during the different stages of lactation. Design: Randomized controlled study. Animals: Fifteen healthy female dromedary camels, with mean body weight of 499.6 kg and mean age of 20 years. Procedures: Camels were categorized into 3 groups' according to their stage of lactation: group 1, early lactation (1-3 months), group 2, mid-lactation (four-6 months) and group3, late lactation (≥ 7 months). Blood samples were collected from every animals for hematological and biochemical evaluation. Results: Total erythrocyte count (TEC), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), total leukocytes (TLC), lymphocytes, neutrophils, monocytes, Calcium, glucose, aspartate aminotransferase (AST), alanine transaminase (ALT), gamma glutamyl transferase (GGT) and alkaline phosphatase (ALP) confirmed significant (p < 0.05) variation between different stages of lactation. However, non-notable (p > 0.05) dissimilarity were located in packed cell volume (PCV), mean corpuscular hemoglobin concentration (MCHC), in organic phosphorus (P), magnesium (Mg), cholesterol, total protein (TP), albumen, globulin, blood urea nitrogen (BUN) and creatinine kinase (CK) in the course of different ranges of lactation, Conclusion and clinical relevance: The results of this investigation may be useful as reference guide for dromedary camel to evaluate the metabolic health status at different stages of lactation.

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Proximate Chemical Analysis, Fatty Acid Profile and Microstructural Characteristics of Dromedary Camel Fats (Hump, Renal and Mesentery)

International Journal of Veterinary Science ◽

10.37422/ijvs/20.014 ◽

2020 ◽

Keyword(s):

Fatty Acid ◽

Fatty Acid Profile ◽

Meat Product ◽

Oxidation Stability ◽

Meat Products ◽

Raw Material ◽

Dromedary Camel ◽

Mean Values ◽

Animal Fat ◽

Mesenteric Fat

The quality, safety, and suitability of animal fat for processing of a specific meat product is a critical issue. Increasing the human awareness about the health aspects associated with increased intake of animal fat, makes camel fat a suitable raw material for meat processing due to its excellent nutritional contribution. Therefore, the target of this study is examination of the sensory, physicochemical, fat oxidation, fatty acid profile, and other quality parameters of camel fat to evaluate the feasibility for processing of different meat products. To achieve this goal, 30 fat samples each from the hump, renal, and mesentery of Arabian male camels were investigated. The results showed that both the renal and mesenteric fat had honey color and medium-soft texture, while the hump had greyish-white color and hard texture. The sensory panel scores were significantly different between the hump and other fats. Hump fat had significantly (P<0.05) higher moisture, protein, and collagen content, while higher fat content was recorded in mesenteric fat. The fatty acid analysis showed that hump had high SFA and very low PUFA in comparison with both renal and mesenteric fat. Camel fat had high oxidation stability, and the mean values were very low in comparison with the levels of quality and acceptability. The ultrastructural analysis showed that hump fat had high elastin fibers which increase its hardness. The results indicated that both renal and mesenteric fat were more suitable for the production of various meat products than the hump.

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Opportunities and challenges related to the implementation of sperm cryopreservation into breeding of salmonid fishes

Theriogenology ◽

10.1016/j.theriogenology.2019.03.022 ◽

2019 ◽

Vol 132 ◽

pp. 12-21 ◽

Cited By ~ 11

Author(s):

Sylwia Judycka ◽

Joanna Nynca ◽

Andrzej Ciereszko

Keyword(s):

Salmonid Fishes ◽

Sperm Cryopreservation

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Sperm Cryopreservation in American Flamingo (Phoenicopterus Ruber): Influence of Cryoprotectants and Seminal Plasma Removal

Animals ◽

10.3390/ani11010203 ◽

2021 ◽

Vol 11 (1) ◽

pp. 203

Author(s):

María Gemma Millán de la Blanca ◽

Eva Martínez-Nevado ◽

Cristina Castaño ◽

Juncal García ◽

Berenice Bernal ◽

...

Keyword(s):

Seminal Plasma ◽

Genetic Material ◽

First Year ◽

Sperm Cryopreservation ◽

Straight Line ◽

The Family ◽

Second Year ◽

American Flamingo ◽

Phoenicopterus Ruber

The American flamingo is a useful model for the development of successful semen cryopreservation procedures to be applied to threatened related species from the family Phoenicopteridae, and to permit genetic material banking. Current study sought to develop effective sperm cryopreservation protocols through examining the influences of two permeating cryoprotectants and the seminal plasma removal. During two consecutive years (April), semen samples were collected and frozen from American flamingos. In the first year, the effect of two permeating cryoprotectants, DMA (dimethylacetamide) (6%) or Me2SO (dimethylsulphoxide) (8%), on frozen–thawed sperm variables were compared in 21 males. No differences were seen between DMA and Me2SO for sperm motility, sperm viability, and DNA fragmentation after thawing. In the second year, the role of seminal plasma on sperm cryoresistance was investigated in 31 flamingos. Sperm samples were cryopreserved with and without seminal plasma, using Me2SO (8%) as a cryoprotectant. The results showed that samples with seminal plasma had higher values than samples without seminal plasma for the following sperm variables: Straight line velocity (22.40 µm/s vs. 16.64 µm/s), wobble (75.83% vs. 69.40%), (p < 0.05), linearity (62.73% vs. 52.01%) and straightness (82.38% vs. 73.79%) (p < 0.01); but acrosome integrity was lower (55.56% vs. 66.88%) (p < 0.05). The cryoresistance ratio (CR) was greater in samples frozen with seminal plasma than without seminal plasma for CR-progressive motility (138.72 vs. 54.59), CR-curvilinear velocity (105.98 vs. 89.32), CR-straight line velocity (152.77 vs. 112.58), CR-average path velocity (122.48 vs. 98.12), CR-wobble (111.75 vs. 102.04) (p < 0.05), CR-linearity (139.41 vs. 113.18), and CR-straightness (124.02 vs. 109.97) (p < 0.01). This research demonstrated that there were not differences between Me2SO and DMA to successful freezing sperm of flamingos; seminal plasma removal did not provide a benefit for sperm cryopreservation.

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Differential Diagnosis of Azoospermia in Men with Infertility

Journal of Clinical Medicine ◽

10.3390/jcm10143144 ◽

2021 ◽

Vol 10 (14) ◽

pp. 3144

Author(s):

Danilo L. Andrade ◽

Marina C. Viana ◽

Sandro C. Esteves

Keyword(s):

Differential Diagnosis ◽

Physical Examination ◽

Detailed Analysis ◽

Clinical Management ◽

Semen Analysis ◽

Testicular Biopsy ◽

Nonobstructive Azoospermia ◽

Sperm Cryopreservation ◽

Imaging Studies ◽

Detailed Medical History

The differential diagnosis between obstructive and nonobstructive azoospermia is the first step in the clinical management of azoospermic patients with infertility. It includes a detailed medical history and physical examination, semen analysis, hormonal assessment, genetic tests, and imaging studies. A testicular biopsy is reserved for the cases of doubt, mainly in patients whose history, physical examination, and endocrine analysis are inconclusive. The latter should be combined with sperm extraction for possible sperm cryopreservation. We present a detailed analysis on how to make the azoospermia differential diagnosis and discuss three clinical cases where the differential diagnosis was challenging. A coordinated effort involving reproductive urologists/andrologists, geneticists, pathologists, and embryologists will offer the best diagnostic path for men with azoospermia.

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