Soil microbial communities responded to biochar application in temperate soils and slowly metabolized13C-labelled biochar as revealed by13C PLFA analyses: results from a short-term incubation and pot experiment

One of the aims of sustainable forest management is to preserve the diversity and resilience of ecosystems. Unfortunately, changes in the soil microbial communities after forest management remain unclear. We analyzed and compared the soil microbial community of a natural Quercus aliena var. acuteserrata forest after four years of four different management methods using high-throughput sequencing technology. The forest management methods were close-to-nature management (CNFM), structure-based forest management (SBFM), secondary forest comprehensive silviculture (SFCS) and unmanaged control (CK). The results showed that: (1) the soil microbial community diversity indices were not significantly different among the different management methods. (2) The relative abundance of Proteobacteria in the SBFM treatment was lower than in the CK treatment, while the relative abundance of Acidobacteria in the SBFM was significantly higher than that in the CK treatment. The relative abundance of Ascomycota was highest in the CNFM treatment, and that of Basidiomycota was lowest in the CNFM treatment. However, the relative abundance of dominant bacterial and fungal phyla was not significantly different in CK and SFCS. (3) Redundancy analysis (RDA) showed that the soil organic matter (SOM), total nitrogen (TN), and available nitrogen (AN) significantly correlated with the bacterial communities, and the available potassium (AK) was the only soil nutrient, which significantly correlated with the composition of the fungal communities. The short-term SBFM treatment altered microbial bacterial community compositions, which may be attributed to the phyla present (e.g., Proteobacteria and Acidobacteria), and the short-term CNFM treatment altered microbial fungal community compositions, which may be attributed to the phyla present (e.g., Ascomycota and Basidiomycota). Furthermore, soil nutrients could affect the dominant soil microbial communities, and its influence was greater on the bacterial community than on the fungal community.

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Short-Term Exposure of Paddy Soil Microbial Communities to Salt Stress Triggers Different Transcriptional Responses of Key Taxonomic Groups

Frontiers in Microbiology ◽

10.3389/fmicb.2017.00400 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 11

Author(s):

Jingjing Peng ◽

Carl-Eric Wegner ◽

Werner Liesack

Keyword(s):

Salt Stress ◽

Microbial Communities ◽

Paddy Soil ◽

Soil Microbial Communities ◽

Short Term ◽

Transcriptional Responses ◽

Soil Microbial ◽

Short Term Exposure ◽

Taxonomic Groups

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Short-term parasite-infection alters already the biomass, activity and functional diversity of soil microbial communities

Scientific Reports ◽

10.1038/srep06895 ◽

2014 ◽

Vol 4 (1) ◽

Cited By ~ 7

Author(s):

Jun-Min Li ◽

Ze-Xin Jin ◽

Frank Hagedorn ◽

Mai-He Li

Keyword(s):

Microbial Communities ◽

Functional Diversity ◽

Soil Microbial Communities ◽

Parasite Infection ◽

Short Term ◽

Soil Microbial ◽

Biomass Activity

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Biochar and Intercropping With Potato–Onion Enhanced the Growth and Yield Advantages of Tomato by Regulating the Soil Properties, Nutrient Uptake, and Soil Microbial Community

Frontiers in Microbiology ◽

10.3389/fmicb.2021.695447 ◽

2021 ◽

Vol 12 ◽

Author(s):

Xingjia He ◽

Hua Xie ◽

Danmei Gao ◽

M. Khashi U. Rahman ◽

Xingang Zhou ◽

...

Keyword(s):

Microbial Community ◽

Microbial Communities ◽

Soil Ph ◽

Fungal Community ◽

Soil Microbial Communities ◽

Pot Experiment ◽

Growth And Yield ◽

Positive Interactions ◽

Soil Microbial ◽

Potato Onion

The application of biochar stimulates the activities of microorganisms that affect soil quality and plant growth. However, studies on the impacts of biochar mainly focus on a monoculture, its effects on interspecific interactions are rarely reported. Here, we investigated the impacts of biochar on tomato/potato–onion intercropped (TO) in a pot experiment. Tomato monoculture (T) and TO were treated with no, 0.3, 0.6, and 1.2% biochar concentrations in a pot experiment. Microbial communities from tomato rhizosphere soil were analyzed by quantitative PCR and Illumina MiSeq. The results showed that compared with the tomato monoculture, 0.6%TO and 1.2%TO significantly increased tomato yield in 2018. TO and 1.2%TO significantly increased plant height and dry weight in 2018 and 2019. Biochar treatments increased soil pH, decreased NO3--N and bulk density, and increased the absorption of N, P, and K by tomato. Bacterial and fungal abundances increased with an increase in biochar concentration, while Bacillus spp. and Pseudomonas spp. abundances showed an “increase-decrease-increase” trend. Biochar had a little effect on bacterial diversities but significantly lowered fungal diversities. TO, 0.6%TO, and 1.2%TO increased the potentially beneficial organisms (e.g., Pseudeurotium and Solirubrobacter) and lowered the potentially pathogenic organisms (e.g., Kribbella and Ilyonectria). Different concentrations of biochar affected the bacterial and fungal community structures. Redundancy analysis indicated that the bacterial community was strongly correlated with soil pH, NO3--N, and EC, while the fungal community was closely related to soil NO3--N and moisture. The network analysis showed that biochar and intercropping affected the symbiosis pattern of the microorganisms and increased the proportion of positive interactions and nitrifying microorganisms (Nitrospirae) in the microbial community. Overall, our results indicated that monoculture and intercropping with biochar improved soil physicochemical states and plant nutrient absorption, and regulated soil microbial communities, these were the main factors to promote tomato growth and increase tomato productivity.

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Long- and short-term temperature differences affect organic and inorganic nitrogen availability in forest soils

Canadian Journal of Soil Science ◽

10.4141/cjss-2014-085 ◽

2015 ◽

Vol 95 (2) ◽

pp. 77-86 ◽

Cited By ~ 2

Author(s):

S. A. Boczulak ◽

B. J. Hawkins ◽

D. G. Maynard ◽

R. Roy

Keyword(s):

Microbial Communities ◽

Forest Soils ◽

Nitrogen Availability ◽

Inorganic Nitrogen ◽

Soil Microbial Communities ◽

Temperature Response ◽

High Elevation ◽

N Cycling ◽

Short Term ◽

Soil Microbial

Boczulak, S. A., Hawkins, B. J., Maynard, D. G. and Roy, R. 2015. Long- and short-term temperature differences affect organic and inorganic nitrogen availability in forest soils. Can. J. Soil Sci. 95: 77–86. Soil microbial activity determines rates of decomposition and is strongly influenced by temperature. Soil microbial communities may be adapted to site characteristics, including temperature, through physiological modification of microbial populations or changes in species composition; however, response to short-term changes in temperature may also occur. We searched for evidence of short- and long-term temperature response of microbial communities involved in soil nitrogen (N) cycling by measuring the relative availability of organic and inorganic N forms in forest soils from a high and a low elevation site, incubated at 10, 16 and 20°C for 16 wk. By week 16, ammonium concentrations were greater in soils incubated at 16 and 20°C than at 10°C, and in soil from the low elevation site, compared with high elevation. Nitrate concentrations increased in soil from the low elevation site incubated at 16 and 20°C, but changed little in other treatments. Assessment of autotrophic nitrification potential showed gross nitrification in soil from the low elevation site was likely from classical chemolithotrophic nitrifiers. Organic N concentration increased over time in the 16 and 20°C incubations of soil from the low elevation site, but only increased in the 20°C treatment for soil from the high elevation site. Long-lasting site effects were indicated by the more active microbial community in soil from low elevation, which could be related to site temperature. Evidence of short-term temperature response of N cycling processes was observed in soils from both elevations.

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