scholarly journals A genomic amplification affecting a carboxylesterase gene cluster confers organophosphate resistance in the mosquito Aedes aegypti from genomic characterization to high‐throughput field detection

2020 ◽  
Author(s):  
Julien Cattel ◽  
Chloé Haberkorn ◽  
Fréderic Laporte ◽  
Thierry Gaude ◽  
Tristan Cumer ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Gene Cluster ◽  
High Throughput ◽  
Genomic Amplification ◽  
Field Detection ◽  
Organophosphate Resistance ◽  
Genomic Characterization

Further genomic characterization of pineapple mealybug wilt-associated viruses using high-throughput sequencing

2020 ◽  
Vol 45 (1) ◽  
pp. 64-72 ◽  
Author(s):  
James C. Green ◽  
Maher A. Rwahnih ◽  
Alejandro Olmedo-Velarde ◽  
Michael J. Melzer ◽  
Islam Hamim ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Genomic Characterization ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

scholarly journals High-Throughput PCR Assays To Monitor Wolbachia Infection in the Dengue Mosquito (Aedes aegypti) and Drosophila simulans

2012 ◽  
Vol 78 (13) ◽  
pp. 4740-4743 ◽  
Author(s):  
Siu F. Lee ◽  
Vanessa L. White ◽  
Andrew R. Weeks ◽  
Ary A. Hoffmann ◽  
Nancy M. Endersby
Keyword(s):  
Aedes Aegypti ◽  
High Throughput ◽  
Large Scale ◽  
Wolbachia Infection ◽  
Drosophila Simulans ◽  
Content Type ◽  
Pcr Assays ◽  
Cost Efficient

ABSTRACTWe have developed and validated two new fluorescence-based PCR assays to detect theWolbachia wMel strain inAedes aegyptiand thewRi andwAu strains inDrosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scaleWolbachiascreening.

scholarly journals Genomic characterization of MDR/XDR-TB in Kazakhstan by a combination of high-throughput methods predominantly shows the ongoing transmission of L2/Beijing 94–32 central Asian/Russian clusters

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
B. J. Klotoe ◽  
S. Kacimi ◽  
E. Costa-Conceicão ◽  
H. M. Gomes ◽  
R. B. Barcellos ◽  
...  
Keyword(s):  
High Throughput ◽  
Central Asian ◽  
Genomic Characterization

High-throughput assays for detection of the F1534C mutation in the voltage-gated sodium channel gene in permethrin-resistant Aedes aegypti and the distribution of this mutation throughout Thailand

2011 ◽  
Vol 16 (4) ◽  
pp. 501-509 ◽  
Author(s):  
Jintana Yanola ◽  
Pradya Somboon ◽  
Catherine Walton ◽  
Woottichai Nachaiwieng ◽  
Puckavadee Somwang ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
High Throughput ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene

scholarly journals A Multipurpose, High-Throughput Single-Nucleotide Polymorphism Chip for the Dengue and Yellow Fever Mosquito, Aedes aegypti

2015 ◽  
Vol 5 (5) ◽  
pp. 711-718 ◽  
Author(s):  
Benjamin R. Evans ◽  
Andrea Gloria-Soria ◽  
Lin Hou ◽  
Carolyn McBride ◽  
Mariangela Bonizzoni ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Aedes Aegypti ◽  
Yellow Fever ◽  
High Throughput ◽  
Yellow Fever Mosquito ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Single Nucleotide Polymorphism Chip

scholarly journals Survey on Non-Human Primates and Mosquitoes Does not Provide Evidences of Spillover/Spillback between the Urban and Sylvatic Cycles of Yellow Fever and Zika Viruses Following Severe Outbreaks in Southeast Brazil

Viruses ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 364 ◽  
Author(s):  
Filipe Vieira Santos de Abreu ◽  
Anielly Ferreira-de-Brito ◽  
Adriana de Souza Azevedo ◽  
José Henrique Rezende Linhares ◽  
Vanessa de Oliveira Santos ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
High Throughput ◽  
Zikv Infection ◽  
Free Living ◽  
Sylvatic Cycle ◽  
Southeast Brazil ◽  
In The Wild ◽  
Current Outbreak ◽  
Time Of Collection

In the last decade, Flaviviruses such as yellow fever (YFV) and Zika (ZIKV) have expanded their transmission areas. These viruses originated in Africa, where they exhibit both sylvatic and interhuman transmission cycles. In Brazil, the risk of YFV urbanization has grown, with the sylvatic transmission approaching the most densely populated metropolis, while concern about ZIKV spillback to a sylvatic cycle has risen. To investigate these health threats, we carried out extensive collections and arbovirus screening of 144 free-living, non-human primates (NHPs) and 5219 mosquitoes before, during, and after ZIKV and YFV outbreaks (2015–2018) in southeast Brazil. ZIKV infection was not detected in any NHP collected at any time. In contrast, current and previous YFV infections were detected in NHPs sampled between 2017 and 2018, but not before the onset of the YFV outbreak. Mosquito pools screened by high-throughput PCR were positive for YFV when captured in the wild and during the YFV outbreak, but were negative for 94 other arboviruses, including ZIKV, regardless of the time of collection. In conclusion, there was no evidence of YFV transmission in coastal southeast Brazil before the current outbreak, nor the spread or establishment of an independent sylvatic cycle of ZIKV or urban Aedes aegypti transmission of YFV in the region. In view of the region’s receptivity and vulnerability to arbovirus transmission, surveillance of NHPs and mosquitoes should be strengthened and continuous.

scholarly journals Insecticide Resistance Status of Aedes aegypti (Diptera: Culicidae) in California by Biochemical Assays

2020 ◽  
Vol 57 (4) ◽  
pp. 1176-1183
Author(s):  
Fan Yang ◽  
Samuel Schildhauer ◽  
Sarah A Billeter ◽  
Melissa Hardstone Yoshimizu ◽  
Robert Payne ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
Southern California ◽  
Mosquito Control ◽  
Central Valley ◽  
Activity Levels ◽  
Metabolic Resistance ◽  
Organophosphate Resistance ◽  
Biochemical Assays ◽  
Effective Use

Abstract Insecticide resistance in Aedes aegypti mosquitoes poses a major threat to public health worldwide. There are two primary biological mechanisms that can lead to insecticide resistance, target site and metabolic resistance, both of which confer resistance to specific classes of insecticides. Due to the limited number of chemical compounds available for mosquito control, it is important to determine current enzymatic profiles among mosquito populations. This study assessed resistance profiles for three metabolic pathways, α-esterases, β-esterases, and mixed-function oxidases (MFOs), as well as insensitivity of the acetylcholinesterase (iAChE) enzyme in the presence of propoxur, among Ae. aegypti from the Central Valley and southern California. All field-collected Ae. aegypti demonstrated elevated MFOs and iAChE activity, indicating potential development of pyrethroid and organophosphate resistance, respectively. Although regional variations were found among α-esterase and β-esterase activity, levels were generally elevated, further suggesting additional mechanisms for developing organophosphate resistance. Furthermore, mosquito samples from southern California exhibited a higher expression level to all three metabolic enzymes and iAChE activity in comparison to mosquitoes from the central region. These results could help guide future mosquito control efforts, directing the effective use of insecticides while limiting the spread of resistance.

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