scholarly journals Lipid moiety of glycosylphosphatidylinositol-anchored proteins contributes to the determination of their final destination in yeast

2018 ◽  
Vol 23 (10) ◽  
pp. 880-892 ◽  
Author(s):  
Takehiko Yoko-o ◽  
Mariko Umemura ◽  
Akiko Komatsuzaki ◽  
Kazutaka Ikeda ◽  
Daisuke Ichikawa ◽  
...  
Keyword(s):  
Final Destination ◽  
Lipid Moiety

scholarly journals Determination of the lipid composition of the GPI anchor

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0256184
Author(s):  
Auxiliadora Aguilera-Romero ◽  
Susana Sabido-Bozo ◽  
Sergio Lopez ◽  
Alejandro Cortes-Gomez ◽  
Sofia Rodriguez-Gallardo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Lipid Composition ◽  
Surface Proteins ◽  
Mass Spectrometry Analysis ◽  
Gpi Anchor ◽  
Spectrometry Analysis ◽  
Lipid Structure ◽  
Lipid Moiety ◽  
Protein Lipidation

In eukaryotic cells, a subset of cell surface proteins is attached by the glycolipid glycosylphosphatidylinositol (GPI) to the external leaflet of the plasma membrane where they play important roles as enzymes, receptors, or adhesion molecules. Here we present a protocol for purification and mass spectrometry analysis of the lipid moiety of individual GPI-anchored proteins (GPI-APs) in yeast. The method involves the expression of a specific GPI-AP tagged with GFP, solubilization, immunoprecipitation, separation by electrophoresis, blotting onto PVDF, release and extraction of the GPI-lipid moiety and analysis by mass spectrometry. By using this protocol, we could determine the precise GPI-lipid structure of the GPI-AP Gas1-GFP in a modified yeast strain. This protocol can be used to identify the lipid composition of the GPI anchor of distinct GPI-APs from yeast to mammals and can be adapted to determine other types of protein lipidation.

scholarly journals PEMODELAN PROSEDUR KARANTINA PENDATANG DALAM RANGKA PENCEGAHAN COVID-19 DI KOTA TARAKAN MENGGUNAKAN PETRI NET

2020 ◽  
Vol 14 (4) ◽  
pp. 587-596
Author(s):  
Deny Murdianto ◽  
Hadi Santoso
Keyword(s):  
Petri Net ◽  
Final Destination ◽  
The Matrix ◽  
The Government

In ordered to prevent the transmission of Covid-19, the government of Tarakan city quarantines immigrants who entered Tarakan city. The government of Tarakan city have created a procedure for implementing the quarantine. Based on these procedures, this article aims to model the quarantine procedure using a Petri net. The Petri net model of the quarantine procedure for immigrants in the context of preventing Covid-19 in Tarakan City consists of eighteen transitions and sixteen places. The evolution of circumstances indicated by the matrix analyzed against the quarantine procedure for domestic immigrants with the final destination of Tarakan city. The determination of marking must be determined seriously so that this quarantine procedure can run smoothly according to purpose. The number of officers must be carefully taken into account so that there is no accumulation of queues of immigrants who was carry out the inspection. The capacity of quarantine places and hospitals must also be well prepared so that they are always available and ready to accept immigrants who was be quarantined.

scholarly journals Avaliação das apreensões e composição química de pasta base apreendidas em Recife – PE

2019 ◽  
Vol 8 (1) ◽  
pp. 24-30
Author(s):  
Antonio Gome de Castro Neto ◽  
Diogo Marques da Silva ◽  
Mialtyan Galindo Freire ◽  
Nadjane teixeira Ribeiro da Silva
Keyword(s):  
Chemical Composition ◽  
Drug Trafficking ◽  
Drug Users ◽  
Consumer Market ◽  
Final Destination ◽  
Demand Reduction ◽  
Cocaine Base ◽  
The Many ◽  
The City

A cocaína é uma das drogas mais consumidas no mundo, sendo o Brasil uma importante rota do tráfico internacional de droga e também um grande mercado consumidor. As várias formas de apresentação da cocaína são feitas a partir da pasta base, a qual também pode ser consumida como droga de abuso. Por passar por diversos atravessadores a pasta base pode sofrer diversas adulterações até chegar ao seu destino final. O objetivo deste trabalho foi avaliar o perfil das apreensões de pasta base na cidade de Recife – PE no período de 2001 a 2015, tendo como base os dados do Instituto de Criminalística Prof. Armando Samico (ICPAS) e também determinar a composição química da droga. Os primeiros casos de apreensão de pasta base ocorreram no ano de 2006 e o ano de 2010 foi o ano que apresentou o maior número de casos de apreensão da droga. A composição química revelou que a pasta base possui em média 51% de cocaína na sua composição e a fenacetina foi o adulterante mais encontrado presente em 31% dos casos. As políticas públicas impactam diretamente nos resultados das apreensões de drogas, sobretudo de pasta base. Mudanças nas políticas de redução da demanda de drogas ao longo dos anos em Pernambuco ocasionou mudança nos perfis das apreensões. A determinação da composição química pode auxiliar tanto no combate ao tráfico como também no tratamento dos usuários de drogas.AbstractCocaine is one of the most consumed drugs in the world, being Brazil an important route of international drug trafficking and also a large consumer market. The many forms of cocaine presentation are made from cocaine base, which can also be consumed as an abuse drug. By passing through several crossings the cocaine base can undergo several adulterations until reaching its final destination. The objective of this work was to evaluate the profile of cocaine base seizures in the city of Recife – PE in the period from 2001 to 2015, based on data of the Institute of Criminalistics Prof. Armando Samico (ICPAS) and also determine the drug chemical composition. The first cases cocaine base seizures occurred in the year of 2006 and the year 2010 presented the largest number of drug seizure cases. Chemical composition revealed that cocaine base has on average 51% cocaine in its composition and phenacetin was the most commonly found adulterant present in 31% of the cases. Public policies have a direct impact on the results of drug seizures, especially of cocaine base. Changes in drug demand reduction policies over the years in Pernambuco have caused changes in seizure patterns. Determination of chemical composition can help both in combating drug trafficking and in drug users treatment. 

Galactic orbits of stars

1966 ◽  
Vol 25 ◽  
pp. 93-97
Author(s):  
Richard Woolley
Keyword(s):  
Globular Cluster ◽  
Potential Field ◽  
High Velocity ◽  
Velocity Vector ◽  
Variable Stars ◽  
The Sun ◽  
Proper Motions ◽  
Rr Lyrae ◽  
The Galaxy

It is now possible to determine proper motions of high-velocity objects in such a way as to obtain with some accuracy the velocity vector relevant to the Sun. If a potential field of the Galaxy is assumed, one can compute an actual orbit. A determination of the velocity of the globular clusterωCentauri has recently been completed at Greenwich, and it is found that the orbit is strongly retrograde in the Galaxy. Similar calculations may be made, though with less certainty, in the case of RR Lyrae variable stars.

Distance to SN 1987A and the LMC

1999 ◽  
Vol 190 ◽  
pp. 549-554
Author(s):  
Nino Panagia
Keyword(s):  
Angular Size ◽  
Large Magellanic Cloud ◽  
Magellanic Cloud ◽  
Light Curves ◽  
Distance Modulus ◽  
Absolute Size ◽  
Sn 1987A

Using the new reductions of the IUE light curves by Sonneborn et al. (1997) and an extensive set of HST images of SN 1987A we have repeated and improved Panagia et al. (1991) analysis to obtain a better determination of the distance to the supernova. In this way we have derived an absolute size of the ringRabs= (6.23 ± 0.08) x 1017cm and an angular sizeR″ = 808 ± 17 mas, which give a distance to the supernovad(SN1987A) = 51.4 ± 1.2 kpc and a distance modulusm–M(SN1987A) = 18.55 ± 0.05. Allowing for a displacement of SN 1987A position relative to the LMC center, the distance to the barycenter of the Large Magellanic Cloud is also estimated to bed(LMC) = 52.0±1.3 kpc, which corresponds to a distance modulus ofm–M(LMC) = 18.58±0.05.

International determination of the total motion of the pole

1961 ◽  
Vol 13 ◽  
pp. 29-41
Author(s):  
Wm. Markowitz
Keyword(s):  
Secular Motion ◽  
The Future

A symposium on the future of the International Latitude Service (I. L. S.) is to be held in Helsinki in July 1960. My report for the symposium consists of two parts. Part I, denoded (Mk I) was published [1] earlier in 1960 under the title “Latitude and Longitude, and the Secular Motion of the Pole”. Part II is the present paper, denoded (Mk II).

Time and Latitude in South Africa

1972 ◽  
Vol 1 ◽  
pp. 27-38
Author(s):  
J. Hers
Keyword(s):  
South Africa ◽  
Cape Town ◽  
Astronomical Observations ◽  
Royal Observatory ◽  
The Republic ◽  
Astronomical Determination ◽  
Limited Accuracy ◽  
Better Than

In South Africa the modern outlook towards time may be said to have started in 1948. Both the two major observatories, The Royal Observatory in Cape Town and the Union Observatory (now known as the Republic Observatory) in Johannesburg had, of course, been involved in the astronomical determination of time almost from their inception, and the Johannesburg Observatory has been responsible for the official time of South Africa since 1908. However the pendulum clocks then in use could not be relied on to provide an accuracy better than about 1/10 second, which was of the same order as that of the astronomical observations. It is doubtful if much use was made of even this limited accuracy outside the two observatories, and although there may – occasionally have been a demand for more accurate time, it was certainly not voiced.

Large-scale Motion of Solar Filaments

2000 ◽  
Vol 179 ◽  
pp. 205-208
Author(s):  
Pavel Ambrož ◽  
Alfred Schroll
Keyword(s):  
Magnetic Flux ◽  
Proper Motion ◽  
Time Scale ◽  
Large Scale ◽  
Accuracy Of Measurements ◽  
Solar Filaments ◽  
General Movement ◽  
Scale Motion ◽  
Velocity Scatter

AbstractPrecise measurements of heliographic position of solar filaments were used for determination of the proper motion of solar filaments on the time-scale of days. The filaments have a tendency to make a shaking or waving of the external structure and to make a general movement of whole filament body, coinciding with the transport of the magnetic flux in the photosphere. The velocity scatter of individual measured points is about one order higher than the accuracy of measurements.

Practical Realization of a Reference System for Earth Dynamics by Satellite Methods

1975 ◽  
Vol 26 ◽  
pp. 341-380 ◽  
Author(s):  
R. J. Anderle ◽  
M. C. Tanenbaum
Keyword(s):  
Reference Frame ◽  
Polar Motion ◽  
Pole Position ◽  
Control Points ◽  
Significant Information ◽  
Crustal Motion ◽  
Average Reference ◽  
Future Data ◽  
Existing Data

AbstractObservations of artificial earth satellites provide a means of establishing an.origin, orientation, scale and control points for a coordinate system. Neither existing data nor future data are likely to provide significant information on the .001 angle between the axis of angular momentum and axis of rotation. Existing data have provided data to about .01 accuracy on the pole position and to possibly a meter on the origin of the system and for control points. The longitude origin is essentially arbitrary. While these accuracies permit acquisition of useful data on tides and polar motion through dynamio analyses, they are inadequate for determination of crustal motion or significant improvement in polar motion. The limitations arise from gravity, drag and radiation forces on the satellites as well as from instrument errors. Improvements in laser equipment and the launch of the dense LAGEOS satellite in an orbit high enough to suppress significant gravity and drag errors will permit determination of crustal motion and more accurate, higher frequency, polar motion. However, the reference frame for the results is likely to be an average reference frame defined by the observing stations, resulting in significant corrections to be determined for effects of changes in station configuration and data losses.

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