The role of Periodic Acid Schiff stain on bone marrow smear to rapid identification of Talaromyces marneffei in a HIV‐positive patient

2020 ◽  
Author(s):  
Yi Xu ◽  
Chen Liu ◽  
Zhi Zhang ◽  
Yueyang Tian ◽  
Jihong Hao
Keyword(s):  
Bone Marrow ◽  
Periodic Acid ◽  
Rapid Identification ◽  
Positive Patient ◽  
Hiv Positive ◽  
Bone Marrow Smear ◽  
Periodic Acid Schiff ◽  
Talaromyces Marneffei

Outcome of a HIV Positive Patient Infected with COVID-19 after an Autologous Bone Marrow Transplantation: A Case Report

2021 ◽  
Vol 19 ◽  
Author(s):  
Masoud Mardani ◽  
Athena Sharifi-Razavi ◽  
Shirin Sheibani ◽  
Mana Baziboroun
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Marrow Transplantation ◽  
Autologous Bone Marrow Transplantation ◽  
Autologous Bone Marrow ◽  
Positive Patient ◽  
World Health ◽  
Hiv Positive ◽  
The World ◽  
Autologous Bone

Background: The World Health Organization (WHO) announced the SARS-COV-2 disease pandemic on March 9, 2020.With the advent of this disease, another health burden was added to about 37.9 million people in the world who are infected with HIV and are suffering from various diseases. These people may be at serious risk of covid-19. Information about the effects of Covid-19 on people living with HIV, is limited. Case presentation: We reported a 61-year-old man who was a known case of HIV from 6 years ago that was being treated with HAART (highly active antiretroviral therapy). He also had a history of Hodgkin’s lymphoma from 4 years ago who underwent autologous bone marrow transplantation (BMT) 2 weeks before given referral to our hospital. He complained of weakness, anorexia, and fever. RT-PCR for SARS-COV-2-RNA was positive in his nasopharyngeal and oropharyngeal swab. He was diagnosed with COVID-19 infection and treated with atazanavir. After one week, the patient discharged in a good general state. Conclusion: To the best of our knowledge, it is the first report of COVID-19 infection in an HIV positive patient after BMT in Iran. Despite his immunodeficiency, COVID-19 disease had mild manifestations and he had a good prognosis. We hope that our report and that of others can remain promising to doctors and HIV patients cross fingers for COVID-19 recovery.

scholarly journals Activation and activity of glycosylated KLKs 3, 4 and 11

2018 ◽  
Vol 399 (9) ◽  
pp. 1009-1022 ◽  
Author(s):  
Shihui Guo ◽  
Peter Briza ◽  
Viktor Magdolen ◽  
Hans Brandstetter ◽  
Peter Goettig
Keyword(s):  
Physiological Function ◽  
Periodic Acid ◽  
Hek293 Cells ◽  
Secretory Expression ◽  
Detailed Characterization ◽  
Periodic Acid Schiff ◽  
Clinical Biomarkers ◽  
Schiff Reaction

Abstract Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process. Notably, these KLKs are upregulated in prostate cancer and are used as clinical biomarkers or have been proposed as therapeutic targets. However, this potential awaits a detailed characterization of these proteases. In order to study glycosylated prostatic KLKs resembling the natural proteases, we used Leishmania (LEXSY) and HEK293 cells for secretory expression. Both systems allowed the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms. Periodic acid-Schiff reaction, enzymatic deglycosylation assays, and mass spectrometry confirmed the glycosylation of these KLKs. Activation of glycosylated pro-KLKs 4 and 11 turned out to be most efficient by glycosylated KLK2 and KLK4, respectively. By comparing the glycosylated prostatic KLKs with their non-glycosylated counterparts from Escherichia coli, it was observed that the N-glycans stabilize the KLK proteases and change their activation profiles and their enzymatic activity to some extent. The functional role of glycosylation in prostate-specific KLKs could pave the way to a deeper understanding of their biology and to medical applications.

Atypical mycobacteria diagnosed by bone marrow biopsy in an HIV-positive patient

2019 ◽  
Vol 99 (1) ◽  
pp. 183-184
Author(s):  
Magda Zanelli ◽  
Maurizio Zizzo ◽  
Giulia Cerrone ◽  
Linda Ricci ◽  
Giovanni Martino ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Biopsy ◽  
Positive Patient ◽  
Atypical Mycobacteria ◽  
Hiv Positive

Clarithromycin inhibits overproduction of muc5ac core protein in murine model of diffuse panbronchiolitis

2003 ◽  
Vol 285 (4) ◽  
pp. L847-L853 ◽  
Author(s):  
Yukihiro Kaneko ◽  
Katsunori Yanagihara ◽  
Masafumi Seki ◽  
Misuzu Kuroki ◽  
Yoshitsugu Miyazaki ◽  
...  
Keyword(s):  
Core Protein ◽  
Periodic Acid ◽  
Protein A ◽  
Mitogen Activated Protein Kinase ◽  
Periodic Acid Schiff ◽  
Western Blots ◽  
Diffuse Panbronchiolitis ◽  
Mucin Production ◽  
Long Term Treatment

Long-term treatment of macrolide antibiotics is considered an effective treatment for diffuse panbronchiolitis (DPB). Although hypersecretion is a common feature of this disease, and it is known that macrolides inhibit mucin production, the mechanism of the effect on mucin production is unclear. The aim of our study was to determine the production of muc5ac core protein, a major core protein of mucin in airway secretion, and the effect of clarithromycin treatment on such production in a mouse model mimicking DPB. Alcian blue-periodic acid-Schiff-positive cells were detected in the lungs of Pseudomonas aeruginosa-infected mice. Western blots of these mice showed muc5ac glycoprotein at day 1 and increased progressively from day 4 to day 14 after inoculation of bacteria. Clarithromycin (10 mg · kg-1· day-1for 7 days) significantly reduced the muc5ac expression at both the mRNA and protein levels. To investigate the role of molecules upstream in muc5ac regulation, we examined the role of mitogen-activated protein kinase. Extracellular signal-regulated kinase 1/2 phosphorylation increased in the infected lung and decreased after treatment. Our results suggest that overproduction of muc5ac plays an important role in the pathogenesis of DPB and that clinical improvement following macrolide therapy seems to involve, at least in part, its inhibition of mucin overproduction, through modulation of intracellular signal transduction.

Systemic Mastocytosis in a Goat

1995 ◽  
Vol 32 (6) ◽  
pp. 719-721 ◽  
Author(s):  
K. N. M. Khan ◽  
J. E. Sagartz ◽  
G. Koenig ◽  
K. Tanaka
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Transmission Electron Microscopy ◽  
Mast Cells ◽  
Systemic Mastocytosis ◽  
Periodic Acid ◽  
Hypochromic Anemia ◽  
Postmortem Examination ◽  
Periodic Acid Schiff ◽  
Transmission Electron

Systemic mastocytosis was diagnosed in a 4-year-old, female Nubian goat. Clinically, the animal was depressed and had severe macrocytic hypochromic anemia and leukopenia. Postmortem examination revealed neoplastic mast cells invading the heart, lung, liver, spleen, lymph nodes, and bone marrow. Eosinophils were frequently admixed with infiltrating mast cells in all organs. Using routine light microscopy, histochemistry, and transmission electron microscopy, metachromatic and periodic acid—Schiff–positive granules were identified within the cytoplasm of neoplastic mast cells. Erythrophagocytosis was observed in some neoplastic cells, although its contribution to the anemia was not clear. This report represents the first description of mast cell neoplasia in the goat.

scholarly journals Mycobacterium genavenseinvading the bone marrow in a HIV-positive patient

2017 ◽  
Vol 5 (6) ◽  
pp. 1043-1045
Author(s):  
Christianne Bourlon ◽  
Cesar Vargas-Serafín ◽  
Xavier López-Karpovitch
Keyword(s):  
Bone Marrow ◽  
Positive Patient ◽  
Hiv Positive

scholarly journals CD8+CD103+ iTregs inhibit the progression of lupus nephritis by attenuating glomerular endothelial cell injury

Rheumatology ◽  
2019 ◽  
Vol 58 (11) ◽  
pp. 2039-2050 ◽  
Author(s):  
Weijuan Deng ◽  
Minwen Xu ◽  
Qiaoyun Meng ◽  
Zhi Li ◽  
Xiaonan Qiu ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Lupus Nephritis ◽  
Inflammatory Mediators ◽  
Cell Injury ◽  
Periodic Acid ◽  
Treg Cells ◽  
Healthy Controls ◽  
Glomerular Endothelial Cell ◽  
Periodic Acid Schiff

Abstract Objectives We previously reported that ex vivo TGF-β and IL-2–induced CD8+CD103+ regulatory T cells (CD8+CD103+ iTregs) displayed similar immunosuppressive effect and therapeutic function on lupus mice nephritis to that of CD4+Foxp3+ Tregs. In view of the important role of glomerular endothelial cell (GEC) injury in inflammatory processes in SLE, this study aimed to investigate the nature and mechanism of CD8+CD103+ iTregs–mediated amelioration of LN by attenuating GEC injury. Methods Treg cells from patients with SLE and from healthy controls were characterized by flow cytometry analysis. The expression of pro-inflammatory mediators and VEGF were analysed in healthy controls, patients with SLE and MRL/lpr mice by ELISA, western blot, and real-time quantitative RT-PCR (qRT-PCR). Typical lesions of diffuse proliferative LN were observed in MRL/lpr mice through the use of haematoxylin and eosin, Masson, periodic acid–Schiff, periodic acid–Schiff methenamine, transmission electron microscopy and IF microscopy. Angiogenesis was analysed in GECs by cell investigating proliferation, migration, and tube formation. Results The results revealed that the frequency of Treg cells was inversely correlated with the expression of VCAM-1 and ICAM-1 in patients with SLE. Furthermore, adoptive transfer of CD8+CD103+ iTregs to MRL/lpr mice was associated with decreased levels of autoantibodies and proteinuria, reduced renal pathological lesions, and lowered renal deposition of IgG/C3. We further found that CD8+CD103+ iTregs not only suppressed the expression of pro-inflammatory mediators but also attenuated GEC injury by promoting angiogenesis. Conclusion Our study has identified the role of CD8+CD103+ iTregs on attenuating GEC injury and provided a possible application of this new iTregs subset in lupus nephritis and other autoimmune diseases.

scholarly journals Endoplasmic Reticulum Chaperon Tauroursodeoxycholic Acid Attenuates Aldosterone-Infused Renal Injury

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Honglei Guo ◽  
Hongmei Li ◽  
Lilu Ling ◽  
Yong Gu ◽  
Wei Ding
Keyword(s):  
Endoplasmic Reticulum ◽  
Inflammatory Response ◽  
Er Stress ◽  
Renal Injury ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Tauroursodeoxycholic Acid ◽  
Receive Treatment ◽  
Inflammatory Molecules

Aldosterone (Aldo) is critically involved in the development of renal injury via the production of reactive oxygen species and inflammation. Endoplasmic reticulum (ER) stress is also evoked in Aldo-induced renal injury. In the present study, we investigated the role of ER stress in inflammation-mediated renal injury in Aldo-infused mice. C57BL/6J mice were randomized to receive treatment for 4 weeks as follows: vehicle infusion, Aldo infusion, vehicle infusion plus tauroursodeoxycholic acid (TUDCA), and Aldo infusion plus TUDCA. The effect of TUDCA on the Aldo-infused inflammatory response and renal injury was investigated using periodic acid-Schiff staining, real-time PCR, Western blot, and ELISA. We demonstrate that Aldo leads to impaired renal function and inhibition of ER stress via TUDCA attenuates renal fibrosis. This was indicated by decreased collagen I, collagen IV, fibronectin, and TGF-β expression, as well as the downregulation of the expression of Nlrp3 inflammasome markers, Nlrp3, ASC, IL-1β, and IL-18. This paper presents an important role for ER stress on the renal inflammatory response to Aldo. Additionally, the inhibition of ER stress by TUDCA negatively regulates the levels of these inflammatory molecules in the context of Aldo.

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