Expression of vanilloid receptor subtype 1 in cutaneous sensory nerve fibers, mast cells, and epithelial cells of appendage structures

2004 ◽  
Vol 13 (3) ◽  
pp. 129-139 ◽  
Author(s):  
Sonja Stander ◽  
Corinna Moormann ◽  
Mark Schumacher ◽  
Jorg Buddenkotte ◽  
Metin Artuc ◽  
...  
Keyword(s):  
Mast Cells ◽  
Epithelial Cells ◽  
Sensory Nerve ◽  
Nerve Fibers ◽  
Vanilloid Receptor ◽  
Receptor Subtype

Functional relationships between sensory nerve fibers and mast cells of dura mater in normal and inflammatory conditions

Neuroscience ◽  
1997 ◽  
Vol 77 (3) ◽  
pp. 829-839 ◽  
Author(s):  
V Dimitriadou ◽  
A Rouleau ◽  
M.D Trung Tuong ◽  
G.J.F Newlands ◽  
H.R.P Miller ◽  
...  
Keyword(s):  
Mast Cells ◽  
Dura Mater ◽  
Sensory Nerve ◽  
Nerve Fibers ◽  
Functional Relationships ◽  
Inflammatory Conditions

Relationships between permeable vessels, nerves, and mast cells in rat cutaneous neurogenic inflammation

1990 ◽  
Vol 68 (6) ◽  
pp. 2305-2311 ◽  
Author(s):  
J. N. Baraniuk ◽  
M. L. Kowalski ◽  
M. A. Kaliner
Keyword(s):  
Electrical Stimulation ◽  
Mast Cells ◽  
Neurogenic Inflammation ◽  
Sensory Nerve ◽  
Nerve Fibers ◽  
Sensory Nerves ◽  
Neurokinin A ◽  
Rat Skin ◽  
Protein Extravasation ◽  
Stimulation Of

Electrical stimulation of rat sensory nerves produces cutaneous vasodilation and plasma protein extravasation, a phenomenon termed “neurogenic inflammation”. Rat skin on the dorsum of the paw developed neurogenic inflammation after electrical stimulation of the saphenous nerve. In tissue sections, the extravasation of the supravital dye monastral blue B identified permeable vessels. Mast cells were identified by toluidine blue stain. Permeable vessels were significantly more dense in the superficial 120 microns of the dermis than in the deeper dermis, whereas mast cells were significantly more frequent in the deeper dermis. The relationships between nociceptive sensory nerve fibers, permeable vessels, and mast cells were examined by indirect immunohistochemistry for calcitonin gene-related peptide (CGRP), neurokinin A (NKA), and substance P (SP). CGRP-, NKA-, and SP-containing nerves densely innervated the superficial dermis and appeared to innervate the vessels that became permeable during neurogenic inflammation. In contrast, mast cells were not associated with either permeable vessels or nerve fibers. These data suggest that electrical stimulation of rat sensory nerves produces vascular permeability by inducing the release of neuropeptides that may directly stimulate the superficial vascular bed. Mast cells may not be involved in this stage of cutaneous neurogenic inflammation in rat skin.

scholarly journals Identification and characterization of a novel human vanilloid receptor-like protein, VRL-2

2001 ◽  
Vol 4 (3) ◽  
pp. 165-174 ◽  
Author(s):  
NATALIE S. DELANY ◽  
MICHAEL HURLE ◽  
PAUL FACER ◽  
TANJA ALNADAF ◽  
CHRISTOPHER PLUMPTON ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Expressed Sequence Tag ◽  
Bipolar Affective Disorder ◽  
Sensory Nerve ◽  
In Silico Analysis ◽  
Human Kidney ◽  
Nerve Fibers ◽  
Vanilloid Receptor ◽  
Sweat Glands ◽  
Parasympathetic Nerve

Remarkable progress has been made recently in identifying a new gene family related to the capsaicin (vanilloid) receptor, VR1. Using a combination of in silico analysis of expressed sequence tag (EST) databases and conventional molecular cloning, we have isolated a novel vanilloid-like receptor, which we call VRL-2, from human kidney. The translated gene shares 46% and 43% identity with VR1 and VRL-1, respectively, and maps to chromosome 12q23–24.1, a locus associated with bipolar affective disorder. VRL-2 mRNA was most strongly expressed in the trachea, kidney, and salivary gland. An affinity-purified antibody against a peptide incorporating the COOH terminal of the receptor localized VRL-2 immunolabel in the distal tubules of the kidney, the epithelial linings of both trachea and lung airways, serous cells of submucosal glands, and mononuclear cells. Unlike VR1 and VRL-1, VRL-2 was not detected in cell bodies of dorsal root ganglia (DRG) or sensory nerve fibers. However, VRL-2 was found on sympathetic and parasympathetic nerve fibers, such as those innervating the arrector pili smooth muscle in skin, sweat glands, intestine, and blood vessels. At least four vanilloid receptor-like genes exist, the newest member, VRL-2 is found in airway and kidney epithelia and in the autonomic nervous system.

Vanilloid Receptor Expression in the Rat Tongue and Palate

2003 ◽  
Vol 82 (5) ◽  
pp. 393-397 ◽  
Author(s):  
M.A. Kido ◽  
H. Muroya ◽  
T. Yamaza ◽  
Y. Terada ◽  
T. Tanaka
Keyword(s):  
Epithelial Cells ◽  
Nerve Fibers ◽  
Vanilloid Receptor ◽  
Receptor Expression ◽  
Burning Pain ◽  
Oral Epithelial Cells ◽  
Oral Pain ◽  
Vanilloid Receptor 1 ◽  
Palatal Rugae ◽  
Hot Peppers

Capsaicin, the pungent substance in hot peppers, evokes a sensation of burning pain by stimulating the vanilloid receptor 1 (VR1) on primary afferent neurons. Immunohistochemistry revealed that the taste papillae in the tongue and palate are richly innervated by VR1-immunoreactive nerve fibers. Furthermore, VR1 protein expression was seen in the epithelium facing the oral cavity, although taste cells seemed to be devoid of VR1. The most conspicuous VR1 expression was observed in the epithelial cells of the palatal rugae, although there were no VR1-immunoreactive nerves there. The finding that VR1 is expressed not only in primary afferents but also in oral epithelial cells suggests that it is of great importance in the perception of capsaicin, heat, and acid in the mouth. Since VR1 is known to play a key role in nociception and inflammatory pain, it may be a new target for the treatment of oral pain.

scholarly journals 425 Mast cells survival and maturation in human skin are regulated and maintained by sensory nerve fibers

2016 ◽  
Vol 136 (5) ◽  
pp. S75 ◽  
Author(s):  
J. Chéret ◽  
L. Ponce ◽  
C. Le Gall-Ianotto ◽  
L. Misery ◽  
M. Bertolini ◽  
...  
Keyword(s):  
Mast Cells ◽  
Human Skin ◽  
Sensory Nerve ◽  
Nerve Fibers

scholarly journals Adventitial Mast Cells Connect With Sensory Nerve Fibers in Atherosclerotic Coronary Arteries

Circulation ◽  
2000 ◽  
Vol 101 (14) ◽  
pp. 1665-1669 ◽  
Author(s):  
P. Laine ◽  
A. Naukkarinen ◽  
L. Heikkila ◽  
A. Penttila ◽  
P. T. Kovanen
Keyword(s):  
Mast Cells ◽  
Coronary Arteries ◽  
Sensory Nerve ◽  
Nerve Fibers

The protease phenotype and crystalline nature of the granules of intraepithelial mast cells in Trichinella spiralis-infected mice

1995 ◽  
Vol 53 ◽  
pp. 818-819
Author(s):  
D.S. Friend ◽  
N. Ghildyal ◽  
M.F. Gurish ◽  
K.F. Austen ◽  
R.L. Stevens
Keyword(s):  
Small Intestine ◽  
Mast Cells ◽  
Epithelial Cells ◽  
Lamina Propria ◽  
Trichinella Spiralis ◽  
Intestinal Epithelial ◽  
Carboxypeptidase A ◽  
C3h Mice ◽  
Granule Morphology ◽  
Crystalline Nature

Trichinella spiralis induces a profound mastocytosis and eosinophilia in the small intestine of the infected mouse. Mouse mast cells (MC) store in their granules various combinations of at least five chymotryptic chymases [designated mouse MC protease (mMCP) 1 to 5], two tryptic proteases designated mMCP-6 and mMCP-7 and an exopeptidase, carboxypeptidase A (mMC-CPA). Using antipeptide, protease -specific antibodies to these MC granule proteases, immunohistochemistry was done to determine the distribution, number and protease phenotype of the MCs in the small intestine and spleen 10 to >60 days after Trichinella infection of BALB/c and C3H mice. TEM was performed to evaluate the granule morphology of the MCs between intestinal epithelial cells and in the lamina propria (mucosal MCs) and in the submucosa, muscle and serosa of the intestine (submucosal MCs).As noted in the table below, the number of submucosal MCs remained constant throughout the study. In contrast, on day 14, the number of MCs in the mucosa increased ~25 fold. Increased numbers of MCs were observed between epithelial cells in the mucosal crypts, in the lamina propria and to a lesser extent, between epithelial cells of the intestinal villi.

scholarly journals Axon typing of rat muscle nerves using a double staining procedure for cholinesterase and carbonic anhydrase.

1991 ◽  
Vol 39 (12) ◽  
pp. 1617-1625 ◽  
Author(s):  
M J Szabolcs ◽  
A Windisch ◽  
R Koller ◽  
M Pensch
Keyword(s):  
Carbonic Anhydrase ◽  
Cross Sections ◽  
Sensory Nerve ◽  
Histological Section ◽  
Trapezius Muscle ◽  
Motor Units ◽  
Conventional Technique ◽  
Nerve Fibers ◽  
Staining Procedure ◽  
Muscle Nerve

We developed a method for detecting activity of axonal cholinesterase (CE) and carbonic anhydrase (CA)--markers for motor and sensory nerve fibers (NFs)--in the same histological section. To reach this goal, cross-sections of muscle nerves were sequentially incubated with the standard protocols for CE and CA histochemistry. A modified incubation medium was used for CA in which Co++ is replaced by Ni++. This avoids interference of the two histochemical reactions because Co++ binds unspecifically to the brown copper-ferroferricyanide complex representing CE activity, whereas Ni++ does not. Cross-sections of the trapezius muscle nerve containing efferent and afferent NFs in segregated fascicles showed that CE activity was confined to motor NFs. Axonal CA was detected solely in sensory NFs. The number of labeled motor and sensory NFs determined in serial cross-sections stained with either the new or the conventional technique was not significantly different. Morphometric analysis revealed that small unreactive NFs (diameter less than 5 microns) are afferent, medium-sized ones (5 microns less than d less than 7 microns) are unclassifiable, and large ones (d greater than 7 microns) are efferent. The heterogenous CE activity of thick (alpha) motor NFs is linked to the type of their motor units. "Fast" motor units contain CE reactive NFs; "slow" ones have CE negative neurites.

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