scholarly journals A Rapid Latex Agglutination Assay for the Detection of Penicillin-Binding Protein 2′

1998 ◽  
Vol 42 (11) ◽  
pp. 739-743 ◽  
Author(s):  
Yasuo Nakatomi ◽  
Junichi Sugiyama
Keyword(s):  
Binding Protein ◽  
Latex Agglutination ◽  
Penicillin Binding Protein ◽  
Agglutination Assay ◽  
Latex Agglutination Assay

scholarly journals Prevalence, Antibiogram and Molecular Characterization of Comunity-Acquired Methicillin-Resistant Staphylococcus Aureus in AWKA, Anambra Nigeria

2016 ◽  
Vol 10 (1) ◽  
pp. 211-221 ◽  
Author(s):  
Blessing Ike ◽  
Malachy C. Ugwu ◽  
Moses N. Ikegbunam ◽  
David Nwobodo ◽  
Chika Ejikeugwu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Binding Protein ◽  
Meca Gene ◽  
Latex Agglutination ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Penicillin Binding Protein ◽  
Carriage Rate ◽  
Molecular Features

Objectives:This study evaluated the prevalence, antibiogram and molecular features of CA-MRSA in Awka, Nigeria.Methods:Confirmation of MRSA was done by testing resistance to oxacillin (1µg), cloxacillin (5µg) and cefoxitin(30µg) on sterile Mueller Hinton agar supplemented with 4% sodium chloride. The MRSA strains were subjected to antimicrobial susceptibility testing using Kirby-Bauer disc diffusion method. Minimum inhibitory concentration was determined using agar dilution method. Penicillin binding protein 2a was detected through rapid latex agglutination assay while mecA gene was detected by polymerase chain reaction. A total of 142S. aureusisolates were obtained from 261 samples sourced from Staff, students and fomites of the Faculty of Pharmaceutical SciencesResult:The overall prevalence of MRSA was 22.6%. The carriage rate was higher in females (56.5%) than male (43.5%) and was highest in individuals of 20-30 years of age (57.65%). The MIC of the oxacillin sodium salt ranged from 4-32 μg/ml. The multi-antibiotic resistance indices show that 53.4% had Multiple Antibiotic Resistance Indexing (MARI) higher than 0.2. Penicillin binding protein 2a was detected in 8.4% of MRSA isolates, all from nasal carriage while mecA gene was detected in 5 of isolates.Conclusion:This study showed a very high prevalence of MRSA carriage among studied subjects.

Toxin Production by Psychrotrophic Bacillus spp. Present in Milk

1990 ◽  
Vol 53 (9) ◽  
pp. 790-792 ◽  
Author(s):  
M. W. GRIFFITHS
Keyword(s):  
Bacillus Cereus ◽  
Brain Heart Infusion ◽  
Bacterial Count ◽  
Toxin Production ◽  
Latex Agglutination ◽  
Brain Heart Infusion Broth ◽  
Agglutination Assay ◽  
Bacillus Spp ◽  
Latex Agglutination Assay

Using a reversed passive latex agglutination assay, about 85% of psychrotrophic Bacillus spp. tested were shown to produce diarrhoegenic toxin during growth on brain heart infusion broth at 25°C. The majority of these strains were identified as Bacillus cereus or cereus-related strains. However, a number of other species was capable of synthesizing the toxin. Further investigation of four psychrotrophic Bacilli showed that the toxin was produced during growth in milk at temperatures ranging from 6 to 21°C. Toxin production increased with increasing temperatures and was not synthesized in appreciable quantities until the bacterial count exceeded 1 × 107 cfu/ml.

A Rapid and Reliable Method for the Detection of Molds in Foods: Using the Latex Agglutination Assay

1989 ◽  
Vol 52 (4) ◽  
pp. 244-247 ◽  
Author(s):  
H. J. KAMPHUIS ◽  
S. NOTERMANS ◽  
G. H. VEENEMAN ◽  
J. H. VAN BOOM ◽  
F. M. ROMBOUTS
Keyword(s):  
Reliable Method ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Penicillium Digitatum ◽  
Agglutination Assay ◽  
Culture Filtrates ◽  
Latex Beads ◽  
Positive Reactivity ◽  
Latex Agglutination Assay

An agglutination test by using latex beads (0.8 μm diameter) coated with IgG from the antibodies against extra-cellular polysaccharide of Penicillium digitatum has been developed. As low as 5 to 10 ng/ml of the purified extra-cellular polysaccharide of the same species can be detected by this preparation. Analysis of culture filtrates from 25 different molds showed that the positive reactivity was obtained only with the species of genera Penicillium and Aspergillus. The application of this test was confirmed in testing the samples of spices and nuts. Further, the reliability could be enhanced by including specific blocker in the assay, the synthetic epitopes consisting of four β (1–5)-linked D-galactofuranosyl residues.

Automated enhanced latex agglutination assay for rheumatoid factors in serum.

1989 ◽  
Vol 35 (2) ◽  
pp. 303-307 ◽  
Author(s):  
J W Winkles ◽  
J Lunec ◽  
L Gray
Keyword(s):  
Rheumatoid Arthritis ◽  
Latex Agglutination ◽  
Rheumatoid Factors ◽  
Agglutination Assay ◽  
Sample Throughput ◽  
Wide Range ◽  
Latex Agglutination Assay

Abstract This improved assay of rheumatoid factors in serum, described here for use with the Baker "Encore" centrifugal analyzer, is efficient, with 250-sample throughput per hour; reproducible, with between-batch CV = 5% and within-batch CV = 2% (mid-assay range); and results correlate well (r = 0.9) with those by other methods. The method is fully quantitative and automated, involves no predilution steps, and can be adapted for use in a wide range of systems. It has a sensitivity of 96% and specificity of 80% in diagnosing rheumatoid arthritis.

Combination of MALDI-TOF MS and PBP2′ latex agglutination assay for rapid MRSA detection

2018 ◽  
Vol 144 ◽  
pp. 122-124 ◽  
Author(s):  
Marianna Ábrók ◽  
Andrea Lázár ◽  
Mária Szécsényi ◽  
Judit Deák ◽  
Edit Urbán
Keyword(s):  
Latex Agglutination ◽  
Agglutination Assay ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms ◽  
Latex Agglutination Assay
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