scholarly journals Quantitative Analysis of BRCA1 and BRCA2 mRNA Expression in Sporadic Breast Carcinomas and Its Relationship with Clinicopathological Characteristics

2001 ◽  
Vol 92 (6) ◽  
pp. 624-630 ◽  
Author(s):  
Chiyomi Egawa ◽  
Yasuo Miyoshi ◽  
Tetsuya Taguchi ◽  
Yasuhiro Tamaki ◽  
Shinzaburo Noguchi
Keyword(s):  
Quantitative Analysis ◽  
Mrna Expression ◽  
Clinicopathological Characteristics ◽  
Breast Carcinomas ◽  
Brca1 And Brca2

Quantitative Analysis of Cytokine mRNA Expression in Hearts from Patients with Nonischemic Dilated Cardiomyopathy (DCM)

2003 ◽  
Vol 18 ◽  
pp. S101-S108 ◽  
Author(s):  
Akira Ukimura ◽  
Fumio Terasaki ◽  
Shigekazu Fujioka ◽  
Hirofumi Deguchi ◽  
Yasushi Kitaura ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Dilated Cardiomyopathy ◽  
Mrna Expression ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression ◽  
Nonischemic Dilated Cardiomyopathy

scholarly journals Multidimensional quantitative analysis of mRNA expression within intact vertebrate embryos

Development ◽  
2018 ◽  
Vol 145 (1) ◽  
pp. dev156869 ◽  
Author(s):  
Vikas Trivedi ◽  
Harry M. T. Choi ◽  
Scott E. Fraser ◽  
Niles A. Pierce
Keyword(s):  
Quantitative Analysis ◽  
Mrna Expression ◽  
Vertebrate Embryos

scholarly journals Quantifying BRCA1 and BRCA2 mRNA Isoform Expression Levels in Single Cells

2019 ◽  
Vol 20 (3) ◽  
pp. 693 ◽  
Author(s):  
Vanessa Lattimore ◽  
John Pearson ◽  
Arthur Morley-Bunker ◽  
kConFab Investigators ◽  
Amanda Spurdle ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Single Cells ◽  
In Situ Hybridisation ◽  
Brca1 And Brca2 ◽  
Mrna Isoforms ◽  
Absolute Expression ◽  
Mrna Isoform ◽  
Splicing Patterns ◽  
Diagnostic Setting

BRCA1 and BRCA2 spliceogenic variants are often associated with an elevated risk of breast and ovarian cancers. Analyses of BRCA1 and BRCA2 splicing patterns have traditionally used technologies that sample a population of cells but do not account for the variation that may be present between individual cells. This novel proof of concept study utilises RNA in situ hybridisation to measure the absolute expression of BRCA1 and BRCA2 mRNA splicing events in single lymphoblastoid cells containing known spliceogenic variants (BRCA1c.671-2 A>G or BRCA2c.7988 A>T). We observed a large proportion of cells (>42%) in each sample that did not express mRNA for the targeted gene. Increased levels (average mRNA molecules per cell) of BRCA2 ∆17_18 were observed in the cells containing the known spliceogenic variant BRCA2c.7988 A>T, but cells containing BRCA1c.671-2 A>G were not found to express significantly increased levels of BRCA1 ∆11, as had been shown previously. Instead, we show for each variant carrier sample that a higher proportion of cells expressed the targeted splicing event compared to control cells. These results indicate that BRCA1/2 mRNA is expressed stochastically, suggesting that previously reported results using RT-PCR may have been influenced by the number of cells with BRCA1/2 mRNA expression and may not represent an elevation of constitutive mRNA expression. Detection of mRNA expression in single cells allows for a more comprehensive understanding of how spliceogenic variants influence the expression of mRNA isoforms. However, further research is required to assess the utility of this technology to measure the expression of predicted spliceogenic BRCA1 and BRCA2 variants in a diagnostic setting.

612 LIVER PD-1/PDL-1/PD-L2 MRNA EXPRESSION QUANTITATIVE ANALYSIS IN PATIENTS WITH CHRONIC HBV AND HCV HEPATITIS

2010 ◽  
Vol 52 ◽  
pp. S241
Author(s):  
N. Argentou ◽  
G. Germanidis ◽  
T. Vassiliadis ◽  
K. Patsiaura ◽  
K. Mantzoukis ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Mrna Expression ◽  
Chronic Hbv

Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders

2014 ◽  
Vol 53 (3) ◽  
pp. 110-117 ◽  
Author(s):  
Flavia Stella ◽  
Estela Pedrazzini ◽  
Edgardo Baialardo ◽  
Dorotea Beatriz Fantl ◽  
Natalia Schutz ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Mrna Expression ◽  
Plasma Cell ◽  
Copy Number ◽  
Copy Number Gain ◽  
Plasma Cell Disorders

Quantitative analysis of survivin mRNA expression in urine and tumor tissue of bladder cancer patients and its potential relevance for disease detection and prognosis

2005 ◽  
Vol 116 (1) ◽  
pp. 100-104 ◽  
Author(s):  
Steffen Weikert ◽  
Frank Christoph ◽  
Mark Schrader ◽  
Hans Krause ◽  
Kurt Miller ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Quantitative Analysis ◽  
Mrna Expression ◽  
Cancer Patients ◽  
Tumor Tissue ◽  
Disease Detection ◽  
Survivin Mrna
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