Genetic mapping by single sperm typing

ABSTRACT The prlC gene of E. coli was originally identified as an allele, prlC1, which suppresses certain signal sequence mutations in the genes for several exported proteins. We have isolated six new alleles of prlC that also confer this phenotype. These mutations can be placed into three classes based on the degree to which they suppress the lamBsignal sequence deletion, lamBs78. Genetic mapping reveals that the physical location of the mutations in prlC correlates with the strength of the suppression, suggesting that different regions of the gene can be altered to yield a suppressor phenotype. We also describe an in vivo cloning procedure using λplacMu9H. The procedure relies on transposition and illegitimate recombination to generate a specialized transducing phage that carries prlC1. This method should be applicable to any gene for which there is a mutant phenotype.

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Human fatty acid synthase mRNA: tissue distribution, genetic mapping, and kinetics of decay after glucose deprivation

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)39738-8 ◽

1995 ◽

Vol 36 (7) ◽

pp. 1507-1521

Author(s):

C F Semenkovich ◽

T Coleman ◽

F T Fiedorek

Keyword(s):

Fatty Acid ◽

Genetic Mapping ◽

Tissue Distribution ◽

Fatty Acid Synthase ◽

Glucose Deprivation ◽

Mrna Tissue Distribution ◽

Kinetics Of

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GENETIC MAPPING OF GENES REQUIRED FOR MOTILITY IN CAULOBACTER CRESCENTUS

Genetics ◽

10.1093/genetics/108.3.523 ◽

1984 ◽

Vol 108 (3) ◽

pp. 523-532

Author(s):

Bert Ely ◽

Ronda H Croft ◽

Connie J Gerardot

Keyword(s):

Genetic Mapping ◽

Caulobacter Crescentus ◽

Pleiotropic Effect ◽

Structure And Function ◽

Bacteriophage Resistance ◽

And Function ◽

Chromosomal Map ◽

Bacterial Systems

ABSTRACT Mutations in more than 30 genes affect motility in Caulobacter crescentus. We have determined the chromosomal map locations for 27 genes involved in flagellar morphogenesis (fla), three genes involved in flagellar function (mot), and three genes that have a pleiotropic effect on both motility and bacteriophage resistance (ple). Three multigene clusters have been detected at widely separated chromosomal locations, but in addition, there are 12 fla and mot genes that are found at eight additional sites scattered around the C. cresentus chromosome. Thus, there is more scatter of genes involved in flagellar structure and function than has been observed in other bacterial systems.

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