IN VITRO LIPID SYNTHESIS IN RAT SKIN

Summary1. Separated leukocytes or total blood from normal subjects, untreated leukaemic patients and from leukaemic patients treated with cytostatic agents were incubated with CH3COONa-l-C14. Radioactivity of mixed lipids was measured at standard time intervals.2. The time incorporation curve observed with leukocytes from treated leukaemic patients showed after an initial linear part, a more rapid levelling off than the curves observed with leukocytes from untreated and normal subjects.3. Therefore, an indirect effect of treatment on leukocyte lipid synthesis seems to be present.4. Phospholipid and neutral lipid synthesis by leukaemic leukocytes was also studied. The results give no evidence that these fractions as a whole have any precursor-product relation.

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Formulation and Evaluation of Carbopol Gel Containing Liposomes of Ketoconazole. (Part-II)

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v1i2.8839 ◽

2009 ◽

Vol 1 (2) ◽

Cited By ~ 1

Author(s):

Rakesh Patel ◽

Hardik Patel ◽

Ashok Baria

Keyword(s):

Antifungal Activity ◽

Rat Skin ◽

Albino Rat ◽

In Vitro Drug Release ◽

Carbopol Gel ◽

Liposomal Formulations ◽

Release Drug ◽

In Vitro Permeation ◽

In Vitro Antifungal Activity

The aim of this work was to prepare and evaluate the topical carbopol gel formulation containing ketoconazole encapsulated liposomes. Ketoconazole loaded liposomes were prepared by thin film hydration technique. The prepared liposomes were incorporated into 1% carbopol gel, and the systems were evaluated for in-vitro drug release, drug retention into skin and in-vitro antifungal activity. The in-vitro permeation of ketoconazole using wistar albino rat skin from liposomal gel was compared with that of plain drug gel and also with plain drug cream containing 2% w/w of ketoconazole. The release of ketoconazole from liposomal gel was much slower than from non liposomal formulations. Gel containing liposomal ketoconazole showed maximum antifungal activity after 30 hours over plain ketoconazole gel and cream formulations.

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The effect of glycerin as penetration enhancer in a ketoprofen solid preparation–patch on in vitro penetration study through rat skin

10.36295/asro.2020.2338 ◽

2020 ◽

Vol 23 (03) ◽

pp. 71-83

Author(s):

Pratama Ferina Nadya ◽

Umam Choirul ◽

Ameliana Lidya ◽

Nurahmanto Dwi

Keyword(s):

Penetration Enhancer ◽

Rat Skin

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Effects of Antimalarial Drugs and Clofibrate on in vitro Lipid Synthesis in Tetrahymena pyriformis GL

Pharmacology ◽

10.1159/000136520 ◽

1974 ◽

Vol 12 (1) ◽

pp. 48-56 ◽

Cited By ~ 5

Author(s):

H.Y.M. Pan ◽

S.C. Chou ◽

K.A. Conklin

Keyword(s):

Lipid Synthesis ◽

Tetrahymena Pyriformis ◽

Antimalarial Drugs

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Sodium alginate nanoparticles as a new transdermal vehicle of glucosamine sulfate for treatment of osteoarthritis

European Journal of Nanomedicine ◽

10.1515/ejnm-2017-0008 ◽

2017 ◽

Vol 9 (3-4) ◽

Cited By ~ 2

Author(s):

Asmaa S. El-Houssiny ◽

Azza A. Ward ◽

Dina M. Mostafa ◽

Salwa L. Abd-El-Messieh ◽

Kamal N. Abdel-Nour ◽

...

Keyword(s):

Side Effects ◽

Surface Charge ◽

Ex Vivo ◽

In Vitro Release ◽

Glucosamine Sulfate ◽

Suspension Stability ◽

Rat Skin ◽

Release Studies ◽

Vitro Release

AbstractGlucosamine sulfate (GS) has been used orally for the treatment of osteoarthritis (OA). However, it may be susceptible to the liver first pass phenomenon, which greatly affects its bioavailability, in addition to its side effects on the gastrointestinal tract. Alginate nanoparticles (Alg NPs) were investigated as a new drug carrier for transdermal delivery of GS to improve its effectiveness and reduce side effects. GS-Alg NPs were characterized by encapsulation efficiency, NP yield, particle size and surface charge properties. The in vitro release studies of GS and the ex vivo permeability through rat skin were determined using a UV-Vis spectrophotometer. GS-Alg NPs are within the nanometer range of size. High negative surface charge values are obtained and indicate the high suspension stability of the prepared formulation. The in vitro release studies showed that GS is released from Alg NPs in a sustained and prolonged manner. The ex vivo permeability of GS through rat skin is enhanced significantly after encapsulation in the negatively charged Alg NPs. We successfully reported a highly stable nanoparticlulate system using Alg NPs that permits the encapsulation of GS for topical administration, overcoming the disadvantages of oral administration.

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