An Epstein-Barr virus-associated lymphoproliferative lesion of the skin presenting as recurrent necrotic papulovesicles of the face

1996 ◽  
Vol 134 (4) ◽  
pp. 791-796 ◽  
Author(s):  
K.H. CHO ◽  
C.W. KIM ◽  
D.Y. LEE ◽  
S.J. SOHN ◽  
D.W. KIM ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Barr Virus ◽  
The Face ◽  
Epstein Barr

Detection of lymphocytes productively infected with Epstein–Barr virus in non-neoplastic tonsils

Microbiology ◽  
2000 ◽  
Vol 81 (5) ◽  
pp. 1211-1216 ◽  
Author(s):  
Tetsuya Ikeda ◽  
Ryo Kobayashi ◽  
Manabu Horiuchi ◽  
Yoshifumi Nagata ◽  
Makoto Hasegawa ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Immune Surveillance ◽  
Lymphoid Tissues ◽  
Healthy Individuals ◽  
Barr Virus ◽  
Multiple Copies ◽  
The Face ◽  
Infected Cells ◽  
Epstein Barr

Epstein–Barr virus (EBV) persists for life in the infected host. Little is known about EBV reactivation and regulation of virus persistence in healthy individuals. We examined tonsils of chronic tonsillitis patients to detect EBV transcripts, EBV genomes and lytic proteins. LMP1 transcripts were observed in 11 of 15 specimens and BZLF1 transcripts were detected in six. Multiple copies of EBV genome equivalents per cell, and ZEBRA- and viral capsid antigen-positive cells were also detected in tonsillar lymphocytes. These results indicate that EBV productively infected cells may survive in the face of immune surveillance in the tonsils. Thus, EBV replication may occur in tonsillar lymphocytes, and tonsillar lymphoid tissues may play a role in the maintenance of EBV load in vivo.

An Epstein-Barr virus-associated lymphoproliferative lesion of the skin presenting as recurrent necrotic papulovesicles of the face

1996 ◽  
Vol 134 (4) ◽  
pp. 791-796 ◽  
Author(s):  
K.H. CHO ◽  
C.W. KIM ◽  
D.Y. LEE ◽  
S.J. SOHN ◽  
D.W. KIM ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Barr Virus ◽  
The Face ◽  
Epstein Barr

scholarly journals Methylation of the Epstein-Barr Virus Genome in Normal Lymphocytes

Blood ◽  
1997 ◽  
Vol 90 (11) ◽  
pp. 4480-4484 ◽  
Author(s):  
Keith D. Robertson ◽  
Richard F. Ambinder
Keyword(s):  
B Cells ◽  
Epstein Barr Virus ◽  
Defense Mechanism ◽  
Immune Surveillance ◽  
Virus Genome ◽  
Barr Virus ◽  
The Face ◽  
Ebv Dna ◽  
Epstein Barr

Abstract Epstein-Barr virus (EBV) latent infection in B cells persists over years or decades despite a sustained cytotoxic immune response to viral antigens. We present data that methylated EBV DNA can be detected in the normal lymphocytes of healthy volunteers. Whereas methylation of foreign DNA has been recognized as a potential cellular defense mechanism, methylation of EBV DNA may be an essential part of the virus life cycle in vivo, explaining the persistence of virus-infected B cells in the face of immune surveillance. Methylation of the C promoter helps to prevent expression of the immunodominant antigens expressed from this promoter. First recognized in tumors, methylation-associated evasion of immune surveillance is not an aberration restricted to tumor tissue but is detected in normal EBV-infected lymphocytes. Methylation of the viral genome in latency also provides an explanation for the CpG suppression associated with EBV but not other large DNA viruses.

Epstein-Barr Virus-Associated Lymphoproliferative Skin Lesion with Recurrent Necrotic Papulovesicles of the Face

1999 ◽  
Vol 26 (7) ◽  
pp. 448-451 ◽  
Author(s):  
Doo Yeoul Jung ◽  
Ji Won Kim ◽  
Sook Kyung Lee ◽  
Won Woo Lee
Keyword(s):  
Skin Lesion ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
The Face ◽  
Epstein Barr

Lymphoepithelioma-like Carcinoma of the Skin

2007 ◽  
Vol 20 (4) ◽  
pp. 851-854 ◽  
Author(s):  
S. Cavalieri ◽  
C. Feliciani ◽  
G. Massi ◽  
G. Addolorato ◽  
G. Gasbarrini ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Epstein Barr Virus ◽  
Malignant Neoplasm ◽  
Barr Virus ◽  
Undifferentiated Cells ◽  
Cutaneous Localization ◽  
The Face ◽  
Poorly Differentiated ◽  
Epstein Barr

The term lymphoepithelioma-like carcinoma identifies a group of nasopharingeal epithelial tumors characterized by aggregates of malignant undifferentiated cells surrounded by a dense reactive lymphoplasmacellular infiltrate. Primary cutaneous localization is rare, with approximately 30 cases reported in literature. We describe a case of primary lymphoepithelioma-like carcinoma of the skin in a 92-year-old woman. Immunohistochemical examination was positive for cytokeratine (KL1 and EMA) as regards epithelial cells, while the lymphocitic infiltrate was positive for LCA and CD3. In situ hybridization for Epstein Barr virus in tumor cells was negative. Electron microscopy showed rounded and occasionally spindle-shaped poorly-differentiated squamous epithelial cells, and a lymphoid stroma consisting mostly of normal-appearing small lymphocytes. Examination of the nasopharynx did not show any tumoral mass and after a 7 years follow-up the patient is free of local and distant recurrences. This tumor affects people aged over 50 years and is localized to the face, but scalp, shoulder and forearm may be involved. Research of Epstein-Barr virus is always negative in this tumor, unlike nasopharingeal epithelial carcinoma. The differential diagnosis of lymphoepithelioma-like carcinoma of the skin may present some difficulties and includes squamous cell carcinoma. Lymphoepithelioma-like carcinoma of the skin is a malignant neoplasm which tends to relapse locally and has a moderate tendency to metastatize.

scholarly journals Methylation of the Epstein-Barr Virus Genome in Normal Lymphocytes

Blood ◽  
1997 ◽  
Vol 90 (11) ◽  
pp. 4480-4484 ◽  
Author(s):  
Keith D. Robertson ◽  
Richard F. Ambinder
Keyword(s):  
B Cells ◽  
Epstein Barr Virus ◽  
Defense Mechanism ◽  
Immune Surveillance ◽  
Virus Genome ◽  
Barr Virus ◽  
The Face ◽  
Ebv Dna ◽  
Epstein Barr

Epstein-Barr virus (EBV) latent infection in B cells persists over years or decades despite a sustained cytotoxic immune response to viral antigens. We present data that methylated EBV DNA can be detected in the normal lymphocytes of healthy volunteers. Whereas methylation of foreign DNA has been recognized as a potential cellular defense mechanism, methylation of EBV DNA may be an essential part of the virus life cycle in vivo, explaining the persistence of virus-infected B cells in the face of immune surveillance. Methylation of the C promoter helps to prevent expression of the immunodominant antigens expressed from this promoter. First recognized in tumors, methylation-associated evasion of immune surveillance is not an aberration restricted to tumor tissue but is detected in normal EBV-infected lymphocytes. Methylation of the viral genome in latency also provides an explanation for the CpG suppression associated with EBV but not other large DNA viruses.

Marked swollen erythema of the face together with sicca syndrome as a sign for chronic active Epstein–Barr virus infection

2000 ◽  
Vol 143 (6) ◽  
pp. 1351-1353 ◽  
Author(s):  
K.C. Sato‐Matsumura ◽  
T. Matsumura ◽  
H. Kobayashi ◽  
K. Fujimoto ◽  
T. Itoh ◽  
...  
Keyword(s):  
Virus Infection ◽  
Epstein Barr Virus ◽  
Sicca Syndrome ◽  
Epstein Barr Virus Infection ◽  
Barr Virus ◽  
The Face ◽  
Epstein Barr ◽  
Barr Virus Infection

A Quantitative Ultrastructural Study of Peripheral Blood Lymphocytes Containing Parallel Tubular Arrays in Epstein-Barr Virus and Cytomegalo-Virus Mononucleosis

1981 ◽  
Vol 39 ◽  
pp. 454-455
Author(s):  
C. M. Payne ◽  
P. M. Tennican
Keyword(s):  
Peripheral Blood ◽  
Lymphocyte Count ◽  
Epstein Barr Virus ◽  
Absolute Lymphocyte Count ◽  
Peripheral Blood Lymphocytes ◽  
Clinical State ◽  
Barr Virus ◽  
The Absolute ◽  
Epstein Barr ◽  
Tubular Arrays

In the normal peripheral circulation there exists a sub-population of lymphocytes which is ultrastructurally distinct. This lymphocyte is identified under the electron microscope by the presence of cytoplasmic microtubular-like inclusions called parallel tubular arrays (PTA) (Figure 1), and contains Fc-receptors for cytophilic antibody. In this study, lymphocytes containing PTA (PTA-lymphocytes) were quantitated from serial peripheral blood specimens obtained from two patients with Epstein -Barr Virus mononucleosis and two patients with cytomegalovirus mononucleosis. This data was then correlated with the clinical state of the patient.It was determined that both the percentage and absolute number of PTA- lymphocytes was highest during the acute phase of the illness. In follow-up specimens, three of the four patients' absolute lymphocyte count fell to within normal limits before the absolute PTA-lymphocyte count.In one patient who was followed for almost a year, the absolute PTA- lymphocyte count was consistently elevated (Figure 2). The estimation of absolute PTA-lymphocyte counts was determined to be valid after a morphometric analysis of the cellular areas occupied by PTA during the acute and convalescent phases of the disease revealed no statistical differences.

Studies of Epstein-Barr Virus Antigens Using Immunoperoxidase and Immunofluorescence Techniques

1975 ◽  
Vol 33 ◽  
pp. 342-343
Author(s):  
R. Stephens ◽  
K. Traul ◽  
D. Woolf ◽  
P. Gaudreau
Keyword(s):  
Electron Microscopy ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
If Technique ◽  
Infected Cells ◽  
Virus Antigens ◽  
Epstein Barr ◽  
Virus Capsid Antigen ◽  
Antigen Reactivity

A number of antigens have been found associated with persistent EBV infections of lymphoblastoid cells. Identification and localization of these antigens were principally by immunofluorescence (IF) techniques using sera from patients with nasopharyngeal carcinoma (NPC), Burkitt lymphoma (BL), and infectious mononucleosis (IM). Our study was mainly with three of the EBV related antigens, a) virus capsid antigen (VCA), b) membrane antigen (MA), and c) early antigens (EA) using immunoperoxidase (IP) techniques with electron microscopy (EM) to elucidate the sites of reactivity with EBV and EBV infected cells.Prior to labeling with horseradish peroxidase (HRP), sera from NPC, IM, and BL cases were characterized for various reactivities by the indirect IF technique. Modifications of the direct IP procedure described by Shabo and the indirect IP procedure of Leduc were made to enhance penetration of the cells and preservation of antigen reactivity.

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