scholarly journals Differential response of human naive and memory/effector T cells to dendritic cells infected by respiratory syncytial virus

2007 ◽  
Vol 150 (2) ◽  
pp. 263-273 ◽  
Author(s):  
T. Rothoeft ◽  
K. Fischer ◽  
S. Zawatzki ◽  
V. Schulz ◽  
U. Schauer ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Respiratory Syncytial Virus ◽  
Differential Response ◽  
Effector T Cells ◽  
Syncytial Virus

scholarly journals Differential Response of Dendritic Cells to Human Metapneumovirus and Respiratory Syncytial Virus

2006 ◽  
Vol 34 (3) ◽  
pp. 320-329 ◽  
Author(s):  
Antonieta Guerrero-Plata ◽  
Antonella Casola ◽  
Giovanni Suarez ◽  
Xiang Yu ◽  
LeAnne Spetch ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Respiratory Syncytial Virus ◽  
Differential Response ◽  
Human Metapneumovirus ◽  
Syncytial Virus

scholarly journals Respiratory Syncytial Virus Infection of Monocyte-Derived Dendritic Cells Decreases Their Capacity to Activate CD4 T Cells

2005 ◽  
Vol 175 (9) ◽  
pp. 5904-5911 ◽  
Author(s):  
Patricia M. A. de Graaff ◽  
Esther C. de Jong ◽  
Toni M. van Capel ◽  
Mariska E. A. van Dijk ◽  
Paul J. M. Roholl ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Respiratory Syncytial Virus ◽  
Virus Infection ◽  
Respiratory Syncytial Virus Infection ◽  
Cd4 T Cells ◽  
Syncytial Virus

scholarly journals Virus-Like Particle Vaccine Containing the F Protein of Respiratory Syncytial Virus Confers Protection without Pulmonary Disease by Modulating Specific Subsets of Dendritic Cells and Effector T Cells

2015 ◽  
Vol 89 (22) ◽  
pp. 11692-11705 ◽  
Author(s):  
Ki-Hye Kim ◽  
Young-Tae Lee ◽  
Hye Suk Hwang ◽  
Young-Man Kwon ◽  
Min-Chul Kim ◽  
...  
Keyword(s):  
Weight Loss ◽  
T Cells ◽  
Dendritic Cells ◽  
Respiratory Syncytial Virus ◽  
Neutralizing Antibodies ◽  
Immune Correlates ◽  
Virus Like Particle ◽  
Tnf Α ◽  
Ifn Γ ◽  
Syncytial Virus

ABSTRACTThere is no licensed vaccine against respiratory syncytial virus (RSV) since the failure of formalin-inactivated RSV (FI-RSV) due to its vaccine-enhanced disease. We investigated immune correlates conferring protection without causing disease after intranasal immunization with virus-like particle vaccine containing the RSV fusion protein (F VLP) in comparison to FI-RSV and live RSV. Upon RSV challenge, FI-RSV immune mice showed severe weight loss, eosinophilia, and histopathology, and RSV reinfection also caused substantial RSV disease despite their viral clearance. In contrast, F VLP immune mice showed least weight loss and no sign of histopathology and eosinophilia. High levels of interleukin-4-positive (IL-4+) and tumor necrosis factor alpha-positive (TNF-α+) CD4+T cells were found in FI-RSV immune mice, whereas gamma interferon-positive (IFN-γ+) and TNF-α+CD4+T cells were predominantly detected in live RSV-infected mice. More importantly, in contrast to FI-RSV and live RSV that induced higher levels of CD11b+dendritic cells, F VLP immunization induced CD8α+and CD103+dendritic cells, as well as F-specific IFN-γ+and TNF-α+CD8+T cells. These results suggest that F VLP can induce protection without causing pulmonary RSV disease by inducing RSV neutralizing antibodies, as well as modulating specific subsets of dendritic cells and CD8 T cell immunity.IMPORTANCEIt has been a difficult challenge to develop an effective and safe vaccine against respiratory syncytial virus (RSV), a leading cause of respiratory disease. Immune correlates conferring protection but preventing vaccine-enhanced disease remain poorly understood. RSV F virus-like particle (VLP) would be an efficient vaccine platform conferring protection. Here, we investigated the protective immune correlates without causing disease after intranasal immunization with RSV F VLP in comparison to FI-RSV and live RSV. In addition to inducing RSV neutralizing antibodies responsible for clearing lung viral loads, we show that modulation of specific subsets of dendritic cells and CD8 T cells producing T helper type 1 cytokines are important immune correlates conferring protection but not causing vaccine-enhanced disease.

scholarly journals Depletion of Regulatory T Cells Induces High Numbers of Dendritic Cells and Unmasks a Subset of Anti-Tumour CD8+CD11c+ PD-1lo Effector T Cells

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157822 ◽  
Author(s):  
Nicolas Goudin ◽  
Pascal Chappert ◽  
Jérome Mégret ◽  
David-Alexandre Gross ◽  
Benedita Rocha ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Regulatory T Cells ◽  
Effector T Cells

scholarly journals Induction of mucosal immunity and protection by intranasal immunization with a respiratory syncytial virus subunit vaccine formulation

2014 ◽  
Vol 95 (2) ◽  
pp. 301-306 ◽  
Author(s):  
R. Garg ◽  
L. Latimer ◽  
E. Simko ◽  
V. Gerdts ◽  
A. Potter ◽  
...  
Keyword(s):  
T Cells ◽  
Respiratory Syncytial Virus ◽  
Lymph Nodes ◽  
Neutralizing Antibodies ◽  
Subunit Vaccine ◽  
Affinity Maturation ◽  
Protein Formulation ◽  
Cross Presentation ◽  
Mucosal Surfaces ◽  
Syncytial Virus

The majority of infections, including those caused by respiratory syncytial virus (RSV), occur at mucosal surfaces. As no RSV vaccine is available our goal is to produce an effective subunit vaccine with an adjuvant suitable for mucosal delivery and cross-presentation. A truncated secreted version of the RSV fusion (ΔF) protein formulated with polyI : C, an innate defence regulator peptide and polyphosphazene, induced local and systemic immunity, including affinity maturation of RSV F-specific IgG, IgA and virus-neutralizing antibodies, and F-specific CD8+ T-cells in the lung, when delivered intranasally. Furthermore, this ΔF protein formulation promoted the production of CD8+ central memory T-cells in the mediastinal lymph nodes and provided protection from RSV challenge. Formulation of ΔF protein with this adjuvant combination enhanced uptake by lung dendritic cells and trafficking to the draining lymph nodes. The ΔF protein formulation was confirmed to be highly efficacious and safe in cotton rats.

scholarly journals Cytotoxic T cells clear virus but augment lung pathology in mice infected with respiratory syncytial virus.

1988 ◽  
Vol 168 (3) ◽  
pp. 1163-1168 ◽  
Author(s):  
M J Cannon ◽  
P J Openshaw ◽  
B A Askonas
Keyword(s):  
T Cells ◽  
Respiratory Syncytial Virus ◽  
T Cell ◽  
Cell Line ◽  
Respiratory Disease ◽  
Cytotoxic T Cells ◽  
Lung Pathology ◽  
Class I Mhc ◽  
Rsv Infection ◽  
Syncytial Virus

We have examined the function of class I MHC-restricted cytotoxic T cells in experimental respiratory syncytial virus (RSV) infection of BALB/c mice by transfer of T cell line MJC-A2 and CTL clone E8a into RSV-infected mice. The T cell line cleared pulmonary RSV infection within 5 d in persistently infected gamma-irradiated mice, but caused acute respiratory disease. This was only seen in infected mice and was often lethal after transfer of greater than 3 x 10(6) CTL. Lower numbers of CTL produced less severe disease but still cleared lung RSV, albeit over a longer time course (up to 10 d). Clearance of lung RSV in immunocompetent mice by the T cell line and CTL clone was again accompanied by acute and sometimes lethal respiratory disease. Bronchoalveolar lavage showed severe lung hemorrhage and frequent neutrophil efflux in mice with CTL-augmented disease.

Sign in / Sign up

Export Citation Format

Share Document