Topographical associations between islet endocrine cells and duct epithelial cells in the adult human pancreas

2008 ◽  
Vol 69 (3) ◽  
pp. 400-406 ◽  
Author(s):  
Hai-Lu Zhao ◽  
Yi Sui ◽  
Jing Guan ◽  
Fernand M. M. Lai ◽  
Xue-Mei Gu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Endocrine Cells ◽  
Human Pancreas ◽  
Adult Human

Differential Integrin Expression Facilitates Isolation of Human Fetal Pancreatic Epithelial Cells

1994 ◽  
Vol 3 (4) ◽  
pp. 307-313 ◽  
Author(s):  
Fred Levine ◽  
Gillian M. Beattie ◽  
Alberto Hayek
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Immunohistochemical Staining ◽  
Endocrine Cells ◽  
Yersinia Pseudotuberculosis ◽  
Affinity Binding ◽  
Β1 Integrin ◽  
Human Pancreas ◽  
High Affinity ◽  
Β1 Integrins

We have studied the expression of the β1 family of integrins in fetal and adult human pancreas. Immunohistochemical staining with a monoclonal anti-β1 antibody revealed that the epithelial cells of the human fetal pancreas express high amounts of β1 integrin, while the pancreatic stromal cells express substantially lower amounts. Islets of Langerhans from human adult pancreas also expressed high amounts of β1 integrin. Taking advantage of the extremely high affinity binding between the invasin protein of Yersinia pseudotuberculosis and many β1 integrins, we have been able to isolate highly enriched populations of fetal pancreatic epithelial cells. Epithelial-enriched cell populations retain the ability to differentiate into mature endocrine cells following transplantation into nude mice.

MicroRNA miR-7 is preferentially expressed in endocrine cells of the developing and adult human pancreas

2009 ◽  
Vol 9 (4) ◽  
pp. 193-199 ◽  
Author(s):  
Mayrin Correa-Medina ◽  
Valia Bravo-Egana ◽  
Samuel Rosero ◽  
Camillo Ricordi ◽  
Helena Edlund ◽  
...  
Keyword(s):  
Endocrine Cells ◽  
Human Pancreas ◽  
Adult Human

Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreas

2006 ◽  
Vol 12 (3) ◽  
pp. 310-316 ◽  
Author(s):  
Ergeng Hao ◽  
Björn Tyrberg ◽  
Pamela Itkin-Ansari ◽  
Jonathan R T Lakey ◽  
Ifat Geron ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Epithelial Cells ◽  
Beta Cell ◽  
Human Pancreas ◽  
Adult Human

scholarly journals Mesenchymal Stem Cells Derived from Human Exocrine Pancreas Spontaneously Express Pancreas Progenitor-Cell Markers in a Cell-Passage-Dependent Manner

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Song Lee ◽  
Seonghee Jeong ◽  
Chanmi Lee ◽  
Jooyun Oh ◽  
Song-Cheol Kim
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Exocrine Pancreas ◽  
Endocrine Cells ◽  
Pancreatic Tissue ◽  
Human Pancreas ◽  
Diabetes Treatment ◽  
Exocrine Cells ◽  
Adult Human ◽  
Pancreatic Endocrine Cells

Mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue, and most connective tissues have been recognized as promising sources for cell-based therapies. MSCs have also been detected in human pancreatic tissue, including endocrine and exocrine cells. These adult human pancreas-derived MSCs have generated a great deal of interest owing to their potential use in the differentiation of insulin-producing cells for diabetes treatment. In the present study, we isolated MSCs from the adult human exocrine pancreas to determine whether isolated MSCs have the potential to differentiate into pancreatic endocrine cells and, therefore, whether they can be used in stem cell-based therapies. Pancreatic tissue was digested by collagenase and an enriched exocrine-cell fraction was obtained by density-gradient separation. Crude exocrine cells were methodically cultured in suspension and then in adherent culture. We expanded the human pancreatic exocrine-derived MSCs (hpMSCs) by cell passaging in culture and confirmed by flow cytometry that >90% expressed human classic surface markers of MSCs. Interestingly, these cells expressed pancreatic transcription factors, such as Pdx1, Ngn3, and MafA, similar to pancreatic progenitor cells. These results indicated that hpMSCs can be used for the differentiation of pancreatic endocrine cells and may be used in type 1 diabetes treatment.

Characterization of Insulin-Immunoreactive Cells and Endocrine Cells Within the Duct System of the Adult Human Pancreas

Pancreas ◽  
2016 ◽  
Vol 45 (5) ◽  
pp. 735-742 ◽  
Author(s):  
Rong Li ◽  
Xiaoxi Zhang ◽  
Lan Yu ◽  
Xia Zou ◽  
Hailu Zhao
Keyword(s):  
Endocrine Cells ◽  
Human Pancreas ◽  
Duct System ◽  
Adult Human

Rapid And Efficient in Vitro Generation of Pancreatic Islet Progenitor Cells from Nonendocrine Epithelial Cells in The Adult Human Pancreas

2007 ◽  
Vol 16 (1) ◽  
pp. 75-90 ◽  
Author(s):  
Josephine S. D'Alessandro ◽  
Kuanghui Lu ◽  
Brenda P. Fung ◽  
Alan Colman ◽  
Diana L. Clarke
Keyword(s):  
Epithelial Cells ◽  
Progenitor Cells ◽  
Pancreatic Islet ◽  
Human Pancreas ◽  
Adult Human

Immunocytochemical study of argyrophil (Sevier Munger) endocrine cells of adult human pancreas

1980 ◽  
Vol 68 (1) ◽  
pp. 95-98 ◽  
Author(s):  
E. Wilander ◽  
M. Lundqvist ◽  
P. Westermark ◽  
L. Grimelius
Keyword(s):  
Endocrine Cells ◽  
Human Pancreas ◽  
Immunocytochemical Study ◽  
Adult Human

Local islet remodelling associated with duct lesion–islet complex in adult human pancreas

Diabetologia ◽  
2021 ◽  
Author(s):  
Yu-Wen Tien ◽  
Hung-Jen Chien ◽  
Tsai-Chen Chiang ◽  
Mei-Hsin Chung ◽  
Chih-Yuan Lee ◽  
...  
Keyword(s):  
Human Pancreas ◽  
Adult Human

Defining conditions to promote the attachment of adult human colonic epithelial cells

1987 ◽  
Vol 23 (6) ◽  
pp. 403-412 ◽  
Author(s):  
Michel Buset ◽  
Sidney Winawer ◽  
Eileen Friedman
Keyword(s):  
Epithelial Cells ◽  
Colonic Epithelial Cells ◽  
Adult Human

scholarly journals Evidence for the presence of stem cell-like progenitor cells in human adult pancreas

2007 ◽  
Vol 195 (3) ◽  
pp. 407-414 ◽  
Author(s):  
Min Zhao ◽  
Stephanie A Amiel ◽  
Michael R Christie ◽  
Paolo Muiesan ◽  
Parthi Srinivasan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Adult Life ◽  
Stem Cell Markers ◽  
Human Pancreas ◽  
Β Cells ◽  
Adult Human ◽  
Pancreatic Cells ◽  
Exocrine Cell ◽  
Cell Fractions ◽  
Unique Cell

The origin of cells replacing ageing β-cells in adult life is unknown. This study assessed the expression of classic stem cell markers: Oct4, Sox2 and CD34 in islet-enriched fractions versus exocrine cell-enriched fractions from 25 adult human pancreases following human islet isolation. Expression of Oct4, Sox2 and CD34 mRNAs was found in all cell samples, with no significant differences between endocrine and exocrine cell fractions. Immunohistochemical staining for Oct4, Sox2, CD133, CD34, CK19, insulin and nestin on human pancreas sections showed that the majority of Oct4+ve cells were found in the walls of small ducts. Similar localisations were observed for Sox2+ve cells. The majority of Sox2+ve cells were found to co-express Oct4 proteins, but not vice versa. Cells positive for Oct4 and Sox2 appeared to be a unique cell population in the adult human pancreases without co-expression for CK19, CD34, CD133, insulin and nestin proteins. The numbers of Oct4+ve and Sox2+ve cells varied among donors and were ∼1–200 and 1–30 per 100 000 pancreatic cells respectively.

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