Expression and clinical significance of glucose transporter 1 mRNA in bronchial brushing liquid-based cytology specimens from patients with and without lung cancer

Background: Liquid-based cytology (LBC) tests, including the liquid-based thin layer method, have demonstrated the highest potential for reducing false-negatives and improving sample quality. Method: This study aimed to evaluate the diagnostic role of LBC of bronchial brushing specimens in lung cancer. A total of 249 patients were analyzed in our study, involving 155 patients with combined bronchial brushing and bronchoalveolar lavage (BAL) and 94 patients with BAL alone. Results: The sensitivity in the combined bronchial brushing and BAL group was 61.4% in the diagnosis of lung cancer, which is much higher than with BAL alone. Rates of positive predictive values and negative predictive values in the combined group compared with the BALF alone group were 98.6% vs 100% and 47.6% vs 37.4%, respectively. Sensitivity in the BALF alone group was 12.5% in bronchoscopically invisible pulmonary lesions and as high as 52.1% in the combined group. Conclusion: The results from our study demonstrated that LBC of brushing samples could be used as an important complement of bronchoscopy and could have the potential to be widely applied.

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Expression and clinical significance of lung-specific X protein mRNA in bronchial brushing specimens from patients with or without lung cancer

Respirology ◽

10.1111/j.1440-1843.2011.02008.x ◽

2011 ◽

Vol 16 (7) ◽

pp. 1076-1080 ◽

Cited By ~ 8

Author(s):

MING LV ◽

MING-ZHE WU ◽

YU-JIE ZHAO ◽

DI YANG ◽

EN-HUA WANG ◽

...

Keyword(s):

Lung Cancer ◽

Clinical Significance ◽

X Protein ◽

Bronchial Brushing

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Salicylketoximes That Target Glucose Transporter 1 Restrict Energy Supply to Lung Cancer Cells

ChemMedChem ◽

10.1002/cmdc.201500320 ◽

2015 ◽

Vol 10 (11) ◽

pp. 1892-1900 ◽

Cited By ~ 15

Author(s):

Carlotta Granchi ◽

Yanrong Qian ◽

Hyang Yeon Lee ◽

Ilaria Paterni ◽

Carolina Pasero ◽

...

Keyword(s):

Lung Cancer ◽

Cancer Cells ◽

Energy Supply ◽

Glucose Transporter ◽

Lung Cancer Cells ◽

Glucose Transporter 1 ◽

Target Glucose

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Clinical Significance of Human Erythrocyte Glucose Transporter 1 Expression at the Deepest Invasive Site of Advanced Colorectal Carcinoma

Oncology ◽

10.1159/000055314 ◽

2001 ◽

Vol 60 (2) ◽

pp. 162-169 ◽

Cited By ~ 35

Author(s):

Akira Furudoi ◽

Shinji Tanaka ◽

Ken Haruma ◽

Masaharu Yoshihara ◽

Koji Sumii ◽

...

Keyword(s):

Colorectal Carcinoma ◽

Clinical Significance ◽

Human Erythrocyte ◽

Glucose Transporter ◽

Glucose Transporter 1 ◽

Advanced Colorectal Carcinoma

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MicroRNA-124 suppresses proliferation and glycolysis in non–small cell lung cancer cells by targeting AKT–GLUT1/HKII

Tumor Biology ◽

10.1177/1010428317706215 ◽

2017 ◽

Vol 39 (5) ◽

pp. 101042831770621 ◽

Cited By ~ 34

Author(s):

Xiaojian Zhao ◽

Caiping Lu ◽

Weiwei Chu ◽

Bing Zhang ◽

Qiang Zhen ◽

...

Keyword(s):

Lung Cancer ◽

Cell Proliferation ◽

Energy Metabolism ◽

Small Cell Lung Cancer ◽

Cancer Cells ◽

Cell Lung Cancer ◽

Glucose Transporter ◽

Small Cell ◽

Small Cell Lung ◽

Glucose Transporter 1

Non–small cell lung cancer accounts for 85% of all types of lung cancer and is the leading cause of worldwide cancer-associated mortalities. MiR-124 is epigenetically silenced in various types of cancer and plays important roles in tumor development and progression. MiR-124 was also significantly downregulated in non–small cell lung cancer patients. Glycolysis has been considered as a feature of cancer cells; hypoxia-inducible factor 1-alpha/beta and Akt are key enzymes in the regulation of glycolysis and energy metabolism in cancer cells. However, the role of miR-124 in non–small cell lung cancer cell proliferation, glycolysis, and energy metabolism remains unknown. In this research, cell proliferation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; furthermore, glucose consumption and lactic acid production were assessed; adenosine triphosphate content and NAD+/NADH were also detected. These tests were conducted using the normal non–small cell lung cancer cell line A549, which was transfected variedly with miR-mimics, miR-124 mimics, miR-124 inhibitor, pc-DNA3.1(+)-AKT1, and pc-DNA3.1(+)-AKT2 plasmid. Here, we show that miR-124 overexpression directly decreased cell growth, glucose consumption, lactate production, and energy metabolism. MiR-124 also negatively regulates glycolysis rate–limiting enzymes, glucose transporter 1 and hexokinase II. Our results also showed that miR-124 negatively regulates AKT1 and AKT2 but no regulatory effect on hypoxia-inducible factor 1-alpha/beta. Overexpression of AKT reverses the inhibitory effect of miR-124 on cell proliferation and glycolytic metabolism in non–small cell lung cancer. AKT inhibition blocks miR-124 silencing–induced AKT1/2, glucose transporter 1, hexokinase II activation, cell proliferation, and glycolytic or energy metabolism changes. In summary, this study demonstrated that miR-124 is able to inhibit proliferation, glycolysis, and energy metabolism, potentially by targeting AKT1/2–glucose transporter 1/hexokinase II in non–small cell lung cancer cells.

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Glucose and Transferrin Liganded PLGA Nanoparticles Internalization in Non-Small Lung Cancer Cells

University of the Future: Re-Imagining Research and Higher Education ◽

10.29117/quarfe.2020.0227 ◽

2020 ◽

Author(s):

Sarra Benammar ◽

Fatima Mraiche ◽

Jensa Mariam Joseph ◽

Katerina Gorachinova

Keyword(s):

Lung Cancer ◽

Cancer Cells ◽

Glucose Transporter ◽

Plga Nanoparticles ◽

Lung Cancer Cells ◽

Size Analysis ◽

Nsclc Cell Line ◽

Glucose Transporter 1 ◽

Cell Internalization ◽

Internalization Rate

Introduction: Recently, after a decade of confusing results, several studies pointed out that overexpression of GLUT1 (glucose transporter 1) is a biomarker of worse prognosis in NSCLC. Nonetheless, the presence of transferrin (Tf receptor), which is overexpressed in most cancer tissues and most lung cancers as well, in NSCLC is also an indicator of very poor prognosis. Therefore, these ligands can be used for active targeting of lung cancer cells and improved efficacy of internalization of cancer therapy using nanomedicines. Objectives: Having the background, the main goal of the project was the assessment of the influence of the glucose and transferrin ligands on the efficacy of internalization of the designed (i) glucose decorated PLGA (poly lactic-coglycolic acid) nanoparticles (Glu-PLGA NPs) and (ii) transferrin decorated PLGA nanoparticles (Tf-PLGA NPs) in comparison to (iii) non-liganded PLGA NPs using a A549 lung cancer cells. Methods: Glu-PLGA NPs, Tf-PLGA NPs and PLGA NP - fluorescently labelled), were designed using a sonication assisted nanoprecipitation method. Further, physicochemical properties characterization (particle size analysis, zeta potential, FTIR analysis, DSC analysis), cytotoxicity evaluation using MTT test, and cell internalization studies of DTAF labelled NPs using fluorimetry in A549 NSCLC cell line were performed. Results: The results pointed to a significantly improved internalization rate of the liganded compared to PLGA NPs. Glu-PLGA NPs showed higher internalization rate compared to Tf-PLGA and PLGA NPs, in the serum-supplemented and serumfree medium even at normal levels of glucose in the cell growth medium. Conclusion: The developed nanocarriers offer unique advantages of enhanced targetability, improved cell internalization and decreased toxicity, which makes them promising solution for current therapeutic limitations.

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β-elemene suppresses Warburg effect in NCI-H1650 non-small-cell lung cancer cells by regulating the miR-301a-3p/AMPKα axis

Bioscience Reports ◽

10.1042/bsr20194389 ◽

2020 ◽

Vol 40 (6) ◽

Author(s):

Lin Li ◽

Dongkai Zhao ◽

Guangyu Cheng ◽

Qingjie Li ◽

Yunjie Chu ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Cell Lung Cancer ◽

Warburg Effect ◽

Glucose Transporter ◽

A549 Cells ◽

Small Cell ◽

Small Cell Lung ◽

Glucose Transporter 1 ◽

The Warburg Effect

Abstract β-elemene has been evidenced to suppress the development of numerous cancers including lung cancer. Previous research has found that in A549 cells, β-elemene increased the expression of adenosine monophosphate-activated protein kinase (AMPK) α (AMPKα), which negatively regulates the Warburg effect. Bioinformatics predicted that binding sites exist between AMPKα and miR-301a-3p, an miRNA that has shown oncogenic function in many cancers. The aim of this work was to investigate the effect of β-elemene on the Warburg effect in non-small-cell lung cancer (NSCLC) cells and its mechanism. Herein, the expression of miR-301a-3p was evaluated in NSCLC cells. Then, miR-301a-3p was overexpressed or silenced by mimics or inhibitors, respectively, followed by treatment with AMPK agonists or antagonists. NSCLC cells subjected to miR-301a-3p overexpression or inhibition were further treated with β-elemene. The results demonstrated that AMPKα was targeted and negatively regulated by miR-301a-3p. AMPKα agonists attenuated the Warburg effect in NSCLC cells induced by miR-301a-3p, as evidenced by the decrease in glucose level, lactic acid level, and expression of metabolism-related enzymes (glucose transporter 1 (GLUT1), hexokinase 1 (HK1), and lactate dehydrogenase A (LDHA)). Additionally, β-elemene suppressed the expression of miR-301a-3p, enhanced that of AMPKα, and inhibited the Warburg effect in NSCLC cells. The results indicated that β-elemene attenuates the Warburg effect in NSCLC cells, possibly by mediating the miR-301a-3p/AMPKα axis.

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