Short-term antibacterial activity of root canal sealers towards Enterococcus faecalis

AbstractEnterococcus faecalis is a biofilm-forming, nosocomial pathogen that is frequently isolated from failed root canal treatments. Contemporary root canal disinfectants are ineffective in eliminating these biofilms and preventing reinfection. As a result, there is a pressing need to identify novel and safe antibiofilm molecules. The effect of short-term (5 and 15 min) and long-term (24 h) treatments of TC on the viability of E. faecalis biofilms was compared with currently used root canal disinfectants. Treatment for 15 min with TC reduced biofilm metabolic activity as effective as 1% sodium hypochlorite and 2% chlorhexidine. Treatment with TC for 24 h was significantly more effective than 2% chlorhexidine in reducing the viable cell counts of biofilms. This serendipitous effect of TC was sustained for 10 days under growth-favoring conditions. For the first time, our study highlights the strong antibacterial activity of TC against E. faecalis biofilms, and notably, its ability to prevent biofilm recovery after treatment.

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Short-Term Antibacterial Efficacy of Three Bioceramic Root Canal Sealers Against Enterococcus Faecalis Biofilms

Acta Stomatologica Croatica ◽

10.15644/asc54/1/1 ◽

2020 ◽

Vol 54 (1) ◽

pp. 3-9

Author(s):

Marija Šimundić Munitić ◽

Ana Budimir ◽

Suzana Jakovljević ◽

Ivica Anić ◽

Ivona Bago

Keyword(s):

Enterococcus Faecalis ◽

Root Canal ◽

Antibacterial Efficacy ◽

Short Term ◽

Root Canal Sealers

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Antibacterial activity of mixed pineapple peel (Ananas comosus) extract and calcium hydroxide paste against Enterococcus faecalis

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v51.i1.p20-24 ◽

2018 ◽

Vol 51 (1) ◽

pp. 20

Author(s):

Intan Fajrin Arsyada ◽

Devi Rianti ◽

Elly Munadziroh

Keyword(s):

Antibacterial Activity ◽

Calcium Hydroxide ◽

Enterococcus Faecalis ◽

Root Canal ◽

Antibacterial Properties ◽

Root Canal Treatment ◽

Ananas Comosus ◽

Treatment Groups ◽

Pineapple Peel ◽

Peel Extract

Background: Enterococcus faecalis (E. faecalis) is the bacteria most commonly resulting from failed root canal treatment. Intracanal medicament is used to enhance the success of root canal treatment. A material widely used for this purpose is calcium hydroxide. However, its ineffectiveness in eliminating E. faecalis requires the addition of other antibacterial substances, such as iodoform which has the disadvantage of having toxic effects on tissues. Pineapple peel has antibacterial properties because it contains chemical compounds, such as flavonoid, saponin, tannin, as well as the enzyme bromelain. Purpose: The aim of the study was to determine the antibacterial activity of a mixture of pineapple peel extract at 6.25% and 12.5% concentrations and calcium hydroxide paste at a ratio of 1:1 compared to100% calcium hydroxide and a mixture of calcium hydroxide and iodoform paste against E. faecalis. Methods: The research was laboratory-based experiment in nature. Sample groups were divided into two control groups (one featuring100% calcium hydroxide paste and a second featuring a mixture of calcium hydroxide and iodoform paste) and two treatment groups (mixture of pineapple peel extract and calcium hydroxide paste in 6.25% and 12.5% concentrations with ratio 1:1). The method was using agar diffusion. The result data were analyzed by One Way Anova test. Results: The highest average of the inhibitory zone occurred in group with a mixture of pineapple peel extract 12.5% and calcium hydroxide paste while the smallest average was that of group with a mixture of calcium hydroxide and iodoform paste. Conclusion: Mixture of pineapple peel extract in 6.25% and 12.5% concentrations and calcium hydroxide paste ratio 1:1 has higher antibacterial activity than paste of 100% calcium hydroxide and mixture of calcium hydroxide and iodoform paste againts E. faecalis.

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Antibacterial activity of two MTA-based root canal sealers

International Endodontic Journal ◽

10.1111/j.1365-2591.2011.01931.x ◽

2011 ◽

Vol 44 (12) ◽

pp. 1128-1133 ◽

Cited By ~ 51

Author(s):

R. D. Morgental ◽

F. V. Vier-Pelisser ◽

S. D. Oliveira ◽

F. C. Antunes ◽

D. M. Cogo ◽

...

Keyword(s):

Antibacterial Activity ◽

Root Canal ◽

Root Canal Sealers

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Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1906 ◽

2016 ◽

Vol 17 (8) ◽

pp. 650-653 ◽

Cited By ~ 1

Author(s):

Jain Mathew ◽

Reshmi George ◽

Robin Theruvil ◽

Tobin C Padavil ◽

Lincy Tomy ◽

...

Keyword(s):

Antibacterial Activity ◽

Enterococcus Faecalis ◽

Sodium Hypochlorite ◽

Root Canal ◽

Leaf Extract ◽

Diffusion Method ◽

Annona Muricata ◽

Root Canals ◽

Root Canal Irrigants ◽

Simarouba Glauca

ABSTRACT Aim To determine the antimicrobial effect of water extracts of leaves of Annona muricata and Simarouba glauca on Enterococcus faecalis using agar diffusion method. Materials and methods Dried leaves of A. muricata and S. glauca were powdered and extracted in a soxhlet apparatus. Enterococcus faecalis was grown overnight in Trypticase soy agar plates. About 10 μL of each extract was placed on agar plates and incubated overnight. The zone of inhibition was measured after 24 hours. About 1% sodium hypochlorite and distilled water were used as positive and negative controls. Results The leaf extract of A. muricata showed similar effectiveness as that of sodium hypochlorite, whereas the leaf extract of S. glauca showed only a slight reduction in growth of E. faecalis. Conclusion Leaf extract of A. muricata can be developed as an alternative to sodium hypochlorite for root canal irrigants. Clinical significance Success of endodontic treatment depends on complete disinfection of the root canals. Root canal irrigants have a major role in complete disinfection of the root canals. Chemical root canal irrigants are more or less toxic to the oral environment. In this study, naturally derived leaf extracts of A. muricata and S. glauca are compared with sodium hypochlorite for its effectiveness against E. faecalis – the most common pathogen found in the root canals. How to cite this article Mathew J, George R, Theruvil R, Padavil TC, Tomy L, Kurian A. Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis. J Contemp Dent Pract 2016;17(8):650-653.

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