Antibacterial activity of different root canal sealers against Enterococcus faecalis

Background: Enterococcus faecalis (E. faecalis) is the bacteria most commonly resulting from failed root canal treatment. Intracanal medicament is used to enhance the success of root canal treatment. A material widely used for this purpose is calcium hydroxide. However, its ineffectiveness in eliminating E. faecalis requires the addition of other antibacterial substances, such as iodoform which has the disadvantage of having toxic effects on tissues. Pineapple peel has antibacterial properties because it contains chemical compounds, such as flavonoid, saponin, tannin, as well as the enzyme bromelain. Purpose: The aim of the study was to determine the antibacterial activity of a mixture of pineapple peel extract at 6.25% and 12.5% concentrations and calcium hydroxide paste at a ratio of 1:1 compared to100% calcium hydroxide and a mixture of calcium hydroxide and iodoform paste against E. faecalis. Methods: The research was laboratory-based experiment in nature. Sample groups were divided into two control groups (one featuring100% calcium hydroxide paste and a second featuring a mixture of calcium hydroxide and iodoform paste) and two treatment groups (mixture of pineapple peel extract and calcium hydroxide paste in 6.25% and 12.5% concentrations with ratio 1:1). The method was using agar diffusion. The result data were analyzed by One Way Anova test. Results: The highest average of the inhibitory zone occurred in group with a mixture of pineapple peel extract 12.5% and calcium hydroxide paste while the smallest average was that of group with a mixture of calcium hydroxide and iodoform paste. Conclusion: Mixture of pineapple peel extract in 6.25% and 12.5% concentrations and calcium hydroxide paste ratio 1:1 has higher antibacterial activity than paste of 100% calcium hydroxide and mixture of calcium hydroxide and iodoform paste againts E. faecalis.

Download Full-text

Antibacterial activity of two MTA-based root canal sealers

International Endodontic Journal ◽

10.1111/j.1365-2591.2011.01931.x ◽

2011 ◽

Vol 44 (12) ◽

pp. 1128-1133 ◽

Cited By ~ 51

Author(s):

R. D. Morgental ◽

F. V. Vier-Pelisser ◽

S. D. Oliveira ◽

F. C. Antunes ◽

D. M. Cogo ◽

...

Keyword(s):

Antibacterial Activity ◽

Root Canal ◽

Root Canal Sealers

Download Full-text

Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1906 ◽

2016 ◽

Vol 17 (8) ◽

pp. 650-653 ◽

Cited By ~ 1

Author(s):

Jain Mathew ◽

Reshmi George ◽

Robin Theruvil ◽

Tobin C Padavil ◽

Lincy Tomy ◽

...

Keyword(s):

Antibacterial Activity ◽

Enterococcus Faecalis ◽

Sodium Hypochlorite ◽

Root Canal ◽

Leaf Extract ◽

Diffusion Method ◽

Annona Muricata ◽

Root Canals ◽

Root Canal Irrigants ◽

Simarouba Glauca

ABSTRACT Aim To determine the antimicrobial effect of water extracts of leaves of Annona muricata and Simarouba glauca on Enterococcus faecalis using agar diffusion method. Materials and methods Dried leaves of A. muricata and S. glauca were powdered and extracted in a soxhlet apparatus. Enterococcus faecalis was grown overnight in Trypticase soy agar plates. About 10 μL of each extract was placed on agar plates and incubated overnight. The zone of inhibition was measured after 24 hours. About 1% sodium hypochlorite and distilled water were used as positive and negative controls. Results The leaf extract of A. muricata showed similar effectiveness as that of sodium hypochlorite, whereas the leaf extract of S. glauca showed only a slight reduction in growth of E. faecalis. Conclusion Leaf extract of A. muricata can be developed as an alternative to sodium hypochlorite for root canal irrigants. Clinical significance Success of endodontic treatment depends on complete disinfection of the root canals. Root canal irrigants have a major role in complete disinfection of the root canals. Chemical root canal irrigants are more or less toxic to the oral environment. In this study, naturally derived leaf extracts of A. muricata and S. glauca are compared with sodium hypochlorite for its effectiveness against E. faecalis – the most common pathogen found in the root canals. How to cite this article Mathew J, George R, Theruvil R, Padavil TC, Tomy L, Kurian A. Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis. J Contemp Dent Pract 2016;17(8):650-653.

Download Full-text

Combined Antibacterial Effect of Sodium Hypochlorite and Root Canal Sealers against Enterococcus faecalis Biofilms in Dentin Canals

Journal of Endodontics ◽

10.1016/j.joen.2015.04.023 ◽

2015 ◽

Vol 41 (8) ◽

pp. 1294-1298 ◽

Cited By ~ 20

Author(s):

Tianfeng Du ◽

Zhejun Wang ◽

Ya Shen ◽

Jingzhi Ma ◽

Yingguang Cao ◽

...

Keyword(s):

Enterococcus Faecalis ◽

Sodium Hypochlorite ◽

Root Canal ◽

Antibacterial Effect ◽

Root Canal Sealers

Download Full-text

Antibacterial Activity of Hybanthus Enneaspermus against Enterococcus Faecalis - A Root Canal Organism

International Journal of Dental Sciences and Research ◽

10.12691/ijdsr-2-6c-4 ◽

2014 ◽

Vol 2 (6C) ◽

pp. 14-16

Author(s):

Kalepu Vamsi ◽

Pradeep Kumar Bholla

Keyword(s):

Antibacterial Activity ◽

Enterococcus Faecalis ◽

Root Canal ◽

Hybanthus Enneaspermus

Download Full-text

An in vitro study on the effects of serum proteins on Enterococcus faecalis adhesion to three types of root sealers and gutta-percha

BMC Oral Health ◽

10.1186/s12903-021-01992-4 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Xinwei Lin ◽

Danlu Chi ◽

Qimei Gong ◽

Zhongchun Tong

Keyword(s):

Epoxy Resin ◽

Calcium Hydroxide ◽

Enterococcus Faecalis ◽

Root Canal ◽

Serum Proteins ◽

Laser Scanning Microscopy ◽

Root Canal Sealer ◽

Gutta Percha ◽

Ah Plus ◽

Root Canal Sealers

Abstract Background The extrusion of overfilled materials that extend beyond the apical foramina into the periradicular tissue may serve as a reservoir for bacterial adhesion and further affect recovery from periapical diseases. The aim of this study was to evaluate the effects of serum proteins on Enterococcus faecalis adhesion and survival on the surface of a calcium hydroxide-based root canal sealer (Apexit Plus), an epoxy resin sealer (AH-Plus) and a bioceramic sealer (iRoot SP). Methods Apexit Plus, AH-Plus and iRoot SP were evenly coated on gutta-percha, using gutta-percha alone as the control. After root canal sealer setting, the number of E. faecalis adhering to the root canal sealers and gutta-percha was counted in fetal bovine serum (FBS) or tryptic soy broth supplemented with 1% glucose (TSBG) by viable cell plate counts. The morphology of 7-day-old E. faecalis biofilms in FSB and TSBG was observed by scanning electron microscopy (SEM). Furthermore, E. faecalis biofilms on the three root canal sealers were labeled with a LIVE/DEAD BacLight™ Bacterial Viability Kit, and the ratios of viable to dead cells were analyzed using laser scanning microscopy operative software (Zen software). Results In the assays, after 1 and 7 days, the number of E. faecalis adhering to the root canal sealers or gutta-percha in FBS were significantly lower than those in TSBG (P < 0.05). In FBS, E. faecalis adhesion to iRoot SP and gutta-percha was reduced to a greater extent than that adhered to Apexit Plus and AH-Plus. Few E. faecalis accumulated on iRoot SP in FBS, whereas many bacteria assembled on iRoot SP and formed biofilms in TSBG. The ratio of viable cells in the E. faecalis biofilm on iRoot SP was the lowest. Conclusions Calcium hydroxide-based root canal sealers, epoxy resin sealers and bioceramic sealers may provide a substrate for E. faecalis adhesion, and the bioceramic sealer in this study showed the least E. faecalis adhesion in the presence of serum proteins compared to the other two sealers.

Download Full-text

Daya Antibakteri Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi linn) terhadap Bakteri Enterococcus faecalis (Antibacterial Activity of Averrhoa bilimbi linn Leaf Extract against Enterococcus Faecalis)

Conservative Dentistry Journal ◽

10.20473/cdj.v6i2.2016.93-98 ◽

2016 ◽

Vol 6 (2) ◽

pp. 93

Author(s):

Ranggi Hardian Nugro Astuti ◽

Karlina Samadi ◽

Eric Priyo Prasetyo

Keyword(s):

Antibacterial Activity ◽

Enterococcus Faecalis ◽

Root Canal ◽

Leaf Extract ◽

Antibacterial Properties ◽

Brain Heart Infusion ◽

Control Group ◽

Control Group Design ◽

Canal Irrigation ◽

Root Canal Irrigation

Background. The prevalence of Enterococcus faecalis bacterial infection caused the failure of root canal treatment between 24%-77%. This is due to various factors resistance and virulence of Enterococcus faecalis. This research to find alternative materials that have antibacterial properties and by utilizing natural ingredients that can later be used as a root canal irrigation. Antibacterial activity of the Averrhoa bilimbi linn leaf extract against Enterococcus faecalis bacteria can be determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Purpose. The aim of this study was to determine the concentration of Averrhoa bilimbi linn leaf extract that has antibacterial activity against bacteria Enterococcus faecalis. Method. This research is a labolatory experimental with post test only control group design which use diluted Enterococcus faecalis ATCC 29212 according Mc. Farland standard 1,5 x 108 CFU/ml. With treatment Averrhoa bilimbi linn leaf extract on concentration 50%, 45%, 40%, 35%, 30%, and 25% given to each of 0,05 ml Enterococcus faecalis and using Brain Heart Infusion Broth (BHIB) as planting media. Result. At the concentration 30% of Averrhoa bilimbi linn leaf extract, showed that colony’s growth less than 10%. At the concentration 35% was not revealed any bacterial growth. Conclusion. The Averrhoa bilimbi linn leaf extract has antibacterial effect on bacteria Enterococcus faecalis. The MIC was at 30% and MBC was at 35%.

Download Full-text