scholarly journals Antibacterial activity of mixed pineapple peel (Ananas comosus) extract and calcium hydroxide paste against Enterococcus faecalis

2018 ◽  
Vol 51 (1) ◽  
pp. 20
Author(s):  
Intan Fajrin Arsyada ◽  
Devi Rianti ◽  
Elly Munadziroh
Keyword(s):  
Antibacterial Activity ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antibacterial Properties ◽  
Root Canal Treatment ◽  
Ananas Comosus ◽  
Treatment Groups ◽  
Pineapple Peel ◽  
Peel Extract

Background: Enterococcus faecalis (E. faecalis) is the bacteria most commonly resulting from failed root canal treatment. Intracanal medicament is used to enhance the success of root canal treatment. A material widely used for this purpose is calcium hydroxide. However, its ineffectiveness in eliminating E. faecalis requires the addition of other antibacterial substances, such as iodoform which has the disadvantage of having toxic effects on tissues. Pineapple peel has antibacterial properties because it contains chemical compounds, such as flavonoid, saponin, tannin, as well as the enzyme bromelain. Purpose: The aim of the study was to determine the antibacterial activity of a mixture of pineapple peel extract at 6.25% and 12.5% concentrations and calcium hydroxide paste at a ratio of 1:1 compared to100% calcium hydroxide and a mixture of calcium hydroxide and iodoform paste against E. faecalis. Methods: The research was laboratory-based experiment in nature. Sample groups were divided into two control groups (one featuring100% calcium hydroxide paste and a second featuring a mixture of calcium hydroxide and iodoform paste) and two treatment groups (mixture of pineapple peel extract and calcium hydroxide paste in 6.25% and 12.5% concentrations with ratio 1:1). The method was using agar diffusion. The result data were analyzed by One Way Anova test. Results: The highest average of the inhibitory zone occurred in group with a mixture of pineapple peel extract 12.5% and calcium hydroxide paste while the smallest average was that of group with a mixture of calcium hydroxide and iodoform paste. Conclusion: Mixture of pineapple peel extract in 6.25% and 12.5% concentrations and calcium hydroxide paste ratio 1:1 has higher antibacterial activity than paste of 100% calcium hydroxide and mixture of calcium hydroxide and iodoform paste againts E. faecalis.

scholarly journals DAYA ANTI BAKTERI EKSTRAK KULIT NANAS (Ananas comosus) TERHADAP PERTUMBUHAN BAKTERI Enterococcus faecalis ANTIBACTERIAL POTENCY OF PINEAPPLE PEEL EXTRACT (Ananas comosus) ON Enterococcus faecalis GROWTH

2016 ◽  
Vol 6 (2) ◽  
pp. 61
Author(s):  
Rega Maurischa Arantika Putri ◽  
Tamara Yuanita ◽  
Mohammad Roelianto
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Positive Control ◽  
Ananas Comosus ◽  
Control Group ◽  
Control Group Design ◽  
Antibacterial Drugs ◽  
Bacteria Growth ◽  
Pineapple Peel ◽  
Peel Extract

Background: Root canal infections is caused by the colonization of microorganisms. Microorganisms that commonly found in root canal failure is Enterococcus faecalis. These bacteria can be found in 80-90% of root canal infections. Currently, many plants are used as antibacterial drugs. Pineapple is one plant that can be used as antibacterial drugs. Pineapple peel has a variety of antibacterial chemical compounds, such as, bromelain, saponins, tannins and flavonoids that can inhibit the growth of Enterococcus faecalis bacteria. Aim: To obtain concentration of antibacterial from pineapple peel extract on Enterococcus faecalis growth. Methods: This study was an experimental laboratory with Post Test Only control group design using Enterococcus faecalis ATCC 29212 bacteria that were diluted based on Mc. Farland standard 1.5 x 108 CFU / ml with pineapple peel extract treatment concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0, 78% and then planted in nutrient agar media surface evenly. Results: At concentration of 3.125% pineapple peel extract showed that the growth of colonies was less than 90% of positive control and concentration of 6.25% pineapple peel extract had no visible Enterococcus faecalis bacteria growth as much as 99.9%. Conclusion: The pineapple peel extract (Ananas comosus) have antibacterial power on the growth of Enterococcus faecalis bacterial colonies with Minimal Inhibitory Concentration (MIC) of 3.125% and the Minimum Bactericidal Concentration (MBC) of 6.25%.

scholarly journals Antibacterial Activity Test of Ethanol Extract Pineapple (Ananas comosus (L.) Merr.) Peel against Growth of Propionibacterium acnes

2019 ◽  
Vol 2 (2) ◽  
pp. 108-113
Author(s):  
Fitriyanti Fitriyanti ◽  
Muhammad Nur Rahman Hendrawan ◽  
Karunita Ika Astuti
Keyword(s):  
Antibacterial Activity ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Ananas Comosus ◽  
Treatment Groups ◽  
Pineapple Peel ◽  
Pineapple Fruit ◽  
Peel Extract

Pineapple peel (Ananas comosus (L.) Merr.) is waste from the pineapple fruit. The purpose of this study was to determine the content of pineapple peel extract and to antibacterial activity in various concentrations effective in inhibiting the growth of Propionibacterium acnes. The pineapple peel extract is made by using the extraction method in the form of maceration. The method used in the inhibitory test using the three replication samples in each treatment group. The sample consisted of 10 treatment groups ie pineapple peel extract concentration 12.5%, 25%, 37.5%, 50%, 62.5%, 75%, 87.5%, and 100%, as well as positive and negative control. The results obtained from pineapple peel screening contain flavonoid and saponin compounds. The data analysis shows that pineapple peel extract concentrations of 50%, 62.5%, 75%, 87.5%, and 100% had inhibitory zones with the medium-strong category while clindamycin as the control has a strong inhibition zone. The conclusion of this research proves that pineapple peel extract has antibacterial power to P. acnes with effective concentration is 100% with the strong category.

scholarly journals Perbedaan Daya Antibakteri Antara Kombinasi Calcium Hydroxide-Based Sealer-Amoxicillin Dan Resin-Based Sealer-Amoxicillin Terhadap Bakteri Enterococcus Faecalis

2019 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Asarizka Bena ◽  
Agus Subiwahjudi ◽  
Setyabudi Setyabudi
Keyword(s):  
Antibacterial Activity ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Control Group ◽  
Control Group Design ◽  
Root Canal Obturation ◽  
Agar Diffusion Test

Background. About 24-77% of root canal treatment failure cases are caused by infection of Enterococcus faecalis due to the resistance factor and virulence of these bacteria. Calcium hydroxide-based sealer and resin-based sealer are two types of sealer often used for root canal obturation due to their antibacterial properties. But the antibacterial properties owned by calcium hydroxide-based sealer has no effect on Enterococcus faecalis. Therefore, the adjunct of local antimicrobial such as amoxicillin is needed to increase antibacterial activities of root canal obturation materials (sealers) especially for calcium hydroxide-based sealer. Purpose. The aim of this study was to compare the effectiveness of antibacterial activity of calcium hydroxide-based sealer-amoxicillin and resin-based sealer-amoxicillin against Enterococcus faecalis. Methods. This study is an experimental laboratory with post test only control group design using Enterococcus faecalis ATCC 29212. Agar diffusion test was used to check the antimicrobial activity of calcium hydroxide-based sealer-amoxicillin and resin-based sealer-amoxicillin. by measuring the inhibition zone diameter of each treatment. Results. Resin-based sealer-amoxicillin’s diameter of zone inhibition is 37,3 mm and calcium hydroxide-based sealer-amoxicillin’s diameter is 18,7 mm. Conclusion. Antibacterial activity of resin-based sealer-amoxicillin is significantly greater than calcium hydroxide-based sealer-amoxicillin.

scholarly journals Antimicrobial properties of various solvents combinations for phytochemical fraction derived from Uncaria gambier extract against Enterococcus faecalis ATCC 29212

2021 ◽  
Vol 33 (1) ◽  
pp. 31
Author(s):  
Gita Dwi Jiwanda Sovira ◽  
Mieke Hemiawati Satari ◽  
Marry Siti Mariam
Keyword(s):  
Ethyl Acetate ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antibacterial Properties ◽  
Density Measurement ◽  
Antimicrobial Properties ◽  
Root Canal Treatment ◽  
Water Fraction ◽  
Root Canal Irrigation ◽  
Mic Value

Introduction: Enterococcus faecalis is often found in root canal infections and can cause failure in root canal treatment. Uncaria gambier is an herbal medicine that is known to be rich in polyphenol compounds that have antibacterial properties. Because of the polyphenol content and antibacterial properties of Uncaria gambier, it can be an alternative as an antibacterial solution for root canal treatment. The purpose of this study is to analyze the antimicrobial of various solvents combinations for phytochemical fraction derived from Uncaria gambier extract against Enterococcus faecalis (ATCC 29212). Methods: This study is descriptive explorative research Uncaria gambier was extracted using a combination of three different fractions, ethyl acetate-water, hexane-water and hexane-ethyl acetate. We determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against Enterococcus faecalis  (ATCC 29212) using the serial microdilution method and by add test samples to the Mueller Hinton agar medium from each fraction Results: Combination ethyl acetate-water has MIC value at 0.195 mg/ml, combination hexane-water at 0.049 mg/ml and combination hexane-ethyl acetate have the MIC value at 1.563 mg/ml. The MBC value in combination ethyl acetate-water at 25 mg/ml, combination hexane-ethyl acetate and hexane-water MBC value at 50 mg/ml and 100 mg/ml. Based on optical density measurement using ELISA reader and bacterial growth on the media used, the fraction combinations increase the antibacterial effect of Uncaria gambier against E. faecalis ATCC 29212. Conclusion: The combination of various Uncaria gambier solvents has been shown to inhibit and kill E. faecalis (ATCC 29212). The combination of ethyl acetate-water fraction is the best combination to against E. faecalis (ATCC 29212). It means that the combination of various Uncaria gambier solvents can be developed as alternative root canal irrigation.

scholarly journals Daya Antibakteri Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi linn) terhadap Bakteri Enterococcus faecalis (Antibacterial Activity of Averrhoa bilimbi linn Leaf Extract against Enterococcus Faecalis)

2016 ◽  
Vol 6 (2) ◽  
pp. 93
Author(s):  
Ranggi Hardian Nugro Astuti ◽  
Karlina Samadi ◽  
Eric Priyo Prasetyo
Keyword(s):  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Brain Heart Infusion ◽  
Control Group ◽  
Control Group Design ◽  
Canal Irrigation ◽  
Root Canal Irrigation

Background. The prevalence of Enterococcus faecalis bacterial infection caused the failure of root canal treatment between 24%-77%. This is due to various factors resistance and virulence of Enterococcus faecalis. This research to find alternative materials that have antibacterial properties and by utilizing natural ingredients that can later be used as a root canal irrigation. Antibacterial activity of the Averrhoa bilimbi linn leaf extract against Enterococcus faecalis bacteria can be determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Purpose. The aim of this study was to determine the concentration of Averrhoa bilimbi linn leaf extract that has antibacterial activity against bacteria Enterococcus faecalis. Method. This research is a labolatory experimental with post test only control group design which use diluted Enterococcus faecalis ATCC 29212 according Mc. Farland standard 1,5 x 108 CFU/ml. With treatment Averrhoa bilimbi linn leaf extract on concentration 50%, 45%, 40%, 35%, 30%, and 25% given to each of 0,05 ml  Enterococcus faecalis and using Brain Heart Infusion Broth (BHIB) as planting media. Result. At the concentration 30% of Averrhoa bilimbi linn leaf extract, showed that colony’s growth less than 10%. At the concentration 35% was not revealed any bacterial growth. Conclusion. The Averrhoa bilimbi linn leaf extract has antibacterial effect on bacteria Enterococcus faecalis. The MIC was at 30% and MBC was at 35%.

scholarly journals Perawatan Ulang Saluran Akar Insisivus Lateralis Kiri Maksila dengan Medikamen Kalsium Hidroksida-Chlorhexidine

2013 ◽  
Vol 20 (1) ◽  
pp. 52
Author(s):  
Ni Gusti Ayu Ariani ◽  
Wignyo Hadriyanto
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal ◽  
Antibacterial Properties ◽  
Root Canal Treatment ◽  
Lateral Incisor ◽  
Radiographic Evaluation ◽  
Periapical Lesion ◽  
Bacterial Endotoxins ◽  
Infected Root ◽  
Maxillary Lateral Incisor

Banyak faktor yang menyebabkan kegagalan terapi endodontik antara lain pembersihan dan membentuk saluran akar yang tidak sempurna dan obturasi tidak hermetis sehingga menyebabkan kurangnya kemampuan untuk menghilangkan mikroorganisme yang ada. Saluran akar yang terinfeksi membutuhkan suatu medikamen untuk menunjang keberhasilan dalam perawatan saluran akar.Kalsium hidroksida merupakan salah satu bahan medikamen yang efektif karena memiliki sifat antibakteri dengan spektrum luas, pH tinggi, biokompatibilitas baik, mampu menetralkan endotoksin bakteri, memiliki sifat toksik yang paling rendah, serta menstimulasi pembentukan jaringan keras. Tujuan laporan kasus untuk menunjukan keberhasilan perawatan ulang saluran akar gigi insisivus lateralis kiri maksila dengan lesi periapikal menggunakan medikamen kalsium hidroksida- chlorhexidine. Pasien wanita umur 53 tahun, gigi insisivus lateralis kiri maksila dengan lesi periapikal.Radiografi tampak obturasi kurang hermetis dan radiolusen daerah periapikal. Perawatan ulang saluran akar,diikuti pemasangan pasak fiber frefabricated dan restorasi porselin fuse metal.Keseimpulan setelah evaluasi setelah enam bulan pasca perawatan ulang saluran akar, radiografi menunjukan radiolusen mengecil dan gigi dapat berfungsi dengan normal.Re-Treatment of Root Canal of Maxillary Left Lateral Incisor with Calcium Hydroxide-Chlorhexidine Medicament. There are many factors that cause failure of endodontic therapy. For instances, incomplete cleaning and shaping of root canal and inadequate obturation that results in difficulty to remove the microorganisms. Infected root canal requires a medicament for the success of the root canal treatment. Calcium hydroxide is one of the effective ingredients as medicament because it has broad spectrum antibacterial properties, high pH, good biocompatibility, and it is able to neutralize bacterial endotoxins, decrease tissue toxicity, and stimulate the formation of hard tissue. The purpose of this case report is to show the success of root canal treatment of the left maxillary lateral incisor with periapical lesions using calcium hydroxide-chlorhexidine medicaments. The patient was a woman aged 53, complaining about her left maxillary lateral incisor with periapical lesion. Based on the radiographic evaluation, there was less hermetic obturation and a radiolucent in the periapical. Root canal re-treatment was continued with fiber prefabricated post and porcelain fused to metal crown. After six months of evaluation and endodontic retreatment, it is found that there is a decrease of radiolucency periapical lesion, and her teeth are able to function normally.

scholarly journals In vitro evaluation of the effectiveness of the chemomechanical preparation against Enterococcus faecalis after single- or multiple-visit root canal treatment

2007 ◽  
Vol 21 (4) ◽  
pp. 308-313 ◽  
Author(s):  
Eduardo Diogo Gurgel-Filho ◽  
Nilton Vivacqua-Gomes ◽  
Brenda Paula Figueiredo de Almeida Gomes ◽  
Caio Cezar Randi Ferraz ◽  
Alexandre Augusto Zaia ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Control Groups ◽  
Single Visit ◽  
Root Canals ◽  
Multiple Visit ◽  
Microbial Samples ◽  
Chlorhexidine Gel

The purpose was to assess the elimination of Enterococcus faecalis in vitro in human mandibular premolars after chemomechanical preparation with or without the use of a calcium hydroxide dressing. After 60 days of contamination with E. faecalis, the root canals were prepared using the Crown-Down technique combined with 2% chlorhexidine gel irrigation. Then, the specimens were divided into two experimental groups, treated in a single visit or in multiple visits, and two control groups. The multiple-visit group received a dressing with calcium hydroxide for 14 days (CalenTM) and the single-visit group did not receive any medication. In the two control groups, the canals were filled with BHI after chemomechanical preparation with 2% chlorhexidine gel or distilled water. Microbial samples were taken from the root canals for colony forming unit count for each phase of the treatment using sterile paper points inside the root canal lumen. Data were ranked and analyzed by the Kruskal-Wallis statistical test. The residual microbial colonies were then assessed. The results showed that chemomechanical preparation using 2% chlorhexidine gel with no intra-canal dressing reduced by 100% the E. faecalis contamination of the root canal lumen. The calcium-hydroxide group that received the 14-day intra-canal dressing allowed a small number of bacteria to grow between visits, but without statistical differences between groups.

scholarly journals Management of periradicular pathosis by single visit endodontic treatment using calcium hydroxide and mineral trioxide aggregate based sealer

2020 ◽  
Vol 10 (2) ◽  
pp. 10-13
Author(s):  
Md Abdul Hannan Sheikh ◽  
Eyad Al Khalifa ◽  
Mozammal Hossain ◽  
Md Faruk Hossain ◽  
Nazneen Karim ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Statistical Methods ◽  
Root Canal ◽  
Mineral Trioxide Aggregate ◽  
Endodontic Treatment ◽  
Root Canal Treatment ◽  
Radiological Evaluation ◽  
Root Canal Therapy ◽  
Radiological Investigation ◽  
Single Visit

In this study, 120 teeth having periradicular pathosis were treated with single visit root canal treatment and then obturated with either calcium hydroxide containing sealer or mineral trioxide aggregate based sealer. The healing of periradicular pathosis was examined at 3, 6, and 12 months by clinical and radiological evaluation. Collected data were analyzed with standard statistical methods by using SPSS version- 20. The results showed that at 3 and 6 months, the differences between calcium hydroxide containing sealer or mineral trioxide aggregate based sealer were not statistically significant. However, the healing capability of periapical radiolucency at 12 months between two groups was statistically significant (P=0.03). The final outcome of the clinical and radiological investigation showed successful results of 96.6% and 98.1% in calcium hydroxide and mineral trioxide aggregate group, respectively. In conclusion, both calcium hydroxide and mineral trioxide aggregate based sealers are almost equally effective in single visit root canal therapy for the management of periradicular pathosis. Update Dent. Coll. j: 2020; 10 (2): 10-13

Root canal treatment with calcium hydroxide

1979 ◽  
Vol 47 (2) ◽  
pp. 185-188 ◽  
Author(s):  
R. Holland ◽  
M.J. Nery ◽  
W. de Mello ◽  
V. de Souza ◽  
P.F.E. Bernabé ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal ◽  
Root Canal Treatment
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