APPLICATION OF HIGH-PRESSURE LIQUID CHROMATOGRAPHY TO THE STUDY OF VARIABLES AFFECTING THEOBROMINE AND CAFFEINE CONCENTRATIONS IN COCOA BEANS

1978 ◽  
Vol 43 (2) ◽  
pp. 560-565 ◽  
Author(s):  
D. J. TIMBIE ◽  
L. SECHRIST ◽  
P. G. KEENEY
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Cocoa Beans

Production of Aflatoxin in Cocoa Beans

1979 ◽  
Vol 62 (5) ◽  
pp. 1076-1079 ◽  
Author(s):  
Lawrence M Lenovich ◽  
W Jeffrey Hurst
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Ivory Coast ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Growth Conditions ◽  
Aflatoxin Contamination ◽  
Cocoa Beans ◽  
Total Aflatoxin ◽  
Substrate Variation

Abstract Aflatoxin was produced in both non-autoclaved and autoclaved Ivory Coast cocoa beans inoculated with Aspergillus parasiticus NRRL 2999 under optimum laboratory growth conditions. Total aflatoxin levels ranged from 213 to 5597 ng/g substrate. Aflatoxin was quantitated by using high pressure liquid chromatography (HPLC). Raw, non-autoclaved cocoa beans, also inoculated with aspergilli, produced 6359 ng aflatoxin/g substrate. Variation in aflatoxin production between bean varieties was observed. Total aflatoxin levels of 10,446 and 23,076 ng/g substrate were obtained on Ivory Coast beans inoculated with A. parasiticus NRRL 2999 and NRRL 3240, respectively. Aflatoxin production on Trinidad and Malaysian beans was 28 and 65 ng aflatoxin/g substrate. These data support previously reported low level natural aflatoxin contamination in cocoa.

Historical chemical annotations of Cinchona bark collections are comparable to results from current day high-pressure liquid chromatography technologies

2020 ◽  
Vol 249 ◽  
pp. 112375 ◽  
Author(s):  
Nataly Allasi Canales ◽  
Tobias Nikolaj Gress Hansen ◽  
Claus Cornett ◽  
Kim Walker ◽  
Felix Driver ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography
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