HEATING EFFECTS OF BOVINE SEMITENDINOSUS: PHASE CONTRAST MICROSCOPY AND SCANNING ELECTRON MICROSCOPY

A suspension of epidermal cells obtained from pig tail skin by trypsinization was subjected to high liquid-shear forces in a French press. The material issuing from the press was examined by phase-contrast microscopy, transmission electron microscopy and scanning electron microscopy. The cytoskeleton of tonofibrils retained the shape of cell fragments, and subcellular organelles remained enmeshed in the network of tonofibrils. Examination of some cell fragments by scanning electron microscopy revealed the internal organization of the tonofibrils. The relevance of these findings to the problem of isolating subcellular fractions from epidermis is discussed.

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Coelomomyces canadense stat. et comb. nov. (Blastocladiales: Coelomomycetaceae) from Psectrocladius sp. G (Diptera: Chironomidae)

Canadian Journal of Botany ◽

10.1139/b78-276 ◽

1978 ◽

Vol 56 (18) ◽

pp. 2303-2306 ◽

Cited By ~ 1

Author(s):

Richard A. Nolan

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Phase Contrast ◽

Field Phase ◽

Species Status ◽

Bright Field ◽

Latin Diagnosis ◽

Taxonomic Criterion ◽

Sporangial Wall ◽

Scanning Electron

Resistant sporangia of Coelomomyces chironomi var. canadense Weiser and McCauley were examined by bright-field, phase-contrast, and scanning electron microscopy (SEM). The use of SEM facilitated the observation of previously undescribed complex furrows in the sporangial wall. The taxonomic criterion for varietal status is discussed, and the variety is elevated to species status. Coelomomyces canadense (Weiser and McCauley) Nolan stat. et comb. nov. is described with an emended Latin diagnosis.

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STUDIES ON THE MODE OF ACTION OF EXCESS OF VITAMIN A

The Journal of Cell Biology ◽

10.1083/jcb.17.1.111 ◽

1963 ◽

Vol 17 (1) ◽

pp. 111-121 ◽

Cited By ~ 48

Author(s):

Audrey M. Glauert ◽

Mary R. Daniel ◽

J. A. Lucy ◽

J. T. Dingle

Keyword(s):

Electron Microscopy ◽

Fine Structure ◽

Vitamin A ◽

Phase Contrast ◽

Phase Contrast Microscopy ◽

Intact Cells ◽

Thin Sections ◽

Initial Effect ◽

Contrast Microscopy ◽

Site Of Action

Rabbit erythrocytes have been haemolysed by treatment with vitamin A alcohol and the sequence of changes in the fine structure of the cells during lysis has been investigated by phase contrast microscopy of intact cells and electron microscopy of thin sections. The initial effect of the vitamin, which occurs within 1 minute, is the production of cells of bizarre appearance which have a greatly increased surface area relative to untreated cells. Large indentations appear in the surfaces of the cells, and vacuoles are formed from the indentations by a process that resembles micropinocytosis. The cells then become spherical and loss of haemoglobin begins as breaks appear in the membranes of some cells; finally, ghosts are produced that are no longer spherical but still contain numerous vacuoles. These observations support the thesis that one site of action of vitamin A is at lipoprotein membranes.

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