Coelomomyces canadense stat. et comb. nov. (Blastocladiales: Coelomomycetaceae) from Psectrocladius sp. G (Diptera: Chironomidae)

1978 ◽  
Vol 56 (18) ◽  
pp. 2303-2306 ◽  
Author(s):  
Richard A. Nolan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phase Contrast ◽  
Field Phase ◽  
Species Status ◽  
Bright Field ◽  
Latin Diagnosis ◽  
Taxonomic Criterion ◽  
Sporangial Wall ◽  
Scanning Electron

Resistant sporangia of Coelomomyces chironomi var. canadense Weiser and McCauley were examined by bright-field, phase-contrast, and scanning electron microscopy (SEM). The use of SEM facilitated the observation of previously undescribed complex furrows in the sporangial wall. The taxonomic criterion for varietal status is discussed, and the variety is elevated to species status. Coelomomyces canadense (Weiser and McCauley) Nolan stat. et comb. nov. is described with an emended Latin diagnosis.

scholarly journals Atractis thapari (Nematoda, Atractidae) parasitizing Chelonoidis carbonarius and C. denticulatus (Testudinidae) in the state of Piauí, Brazil

2018 ◽  
Vol 27 (2) ◽  
pp. 146-153
Author(s):  
Anangela Ravena da Silva Leal ◽  
Simone Mousinho Freire ◽  
Marcelo Knoff ◽  
Delir Corrêa Gomes ◽  
Jeannie Nascimento dos Santos ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Neotropical Region ◽  
Health Conditions ◽  
Taxonomic Identification ◽  
Bright Field ◽  
New Host ◽  
Male And Female ◽  
Mean Intensity ◽  
Scanning Electron

Abstract Atractis thapari Petter, 1966, an atractid nematode, was collected parasitizing the large intestine of tortoises of the species Chelonoidis carbonarius (Spix, 1824) (Cc) and C. denticulatus (Linnaeus, 1766) (Cd) (Testudinidae) in the Zoobotanical Park of the municipality of Teresina, state of Piauí, Brazil. Taxonomic identification was based on morphological and morphometrical features, and using bright-field and scanning electron microscopy. The present study adds new observations on the morphology of A. thapari, mainly relating to mouth papillae, the excretory pore, deirids, and male and female posterior ends. The parasitic indices of prevalence (P), mean intensity (MI), mean abundance (MA) and range of infection (RI) for A. thapari in these two tortoise species were: P = 100%, MI = 154,667, MA = 154,667, RI = 5,500-588,500 (Cc); P = 100%, MI = 93,639, MA = 93,639, RI = 1,000-224,500 (Cd). This report confirms the occurrence of A. thapari in Neotropical region, South America, Brazil, and extends its occurrences to a new host, the tortoise C. carbonarius. Adjustment of host management with the aim of improving hygiene and health conditions is suggested.

Fine morphology of the cuticle surface of Chordodes anthophorus and reinterpretation of C. aquaeductus, C. ferganensis and C. oscillatus (Gordiida Nematomorpha)

Zootaxa ◽  
2007 ◽  
Vol 1397 (1) ◽  
pp. 39-45 ◽  
Author(s):  
CRISTINA DE VILLALOBOS ◽  
FERNANDA ZANCA ◽  
SERGEI SPIRIDONOV
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Central Asia ◽  
The Other ◽  
Species Status ◽  
Taxonomic Descriptions ◽  
Former Ussr ◽  
Fine Morphology ◽  
Scanning Electron

We reinvestigate four species of the genus Chordodes from Central Asia and one from Georgia (Transcaucasian part of former USSR) in order to certify the taxonomic descriptions and verify the species status. By scanning electron microscopy, we demonstrate characteristic cuticular patterns for Chordodes anthophorus. The other three species, C. aquaeductus, C. ferganensis and C. oscillatus are synonymized with C. anthophorus because no differences were detected.

An Inexpensive Approach for Bright-Field and Dark-Field Imaging by Scanning Transmission Electron Microscopy in Scanning Electron Microscopy

2014 ◽  
Vol 20 (1) ◽  
pp. 124-132 ◽  
Author(s):  
Binay Patel ◽  
Masashi Watanabe
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Scanning Transmission Electron Microscopy ◽  
Lattice Spacing ◽  
Dark Field ◽  
Bright Field ◽  
Specimen Holder ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Scanning Electron

AbstractScanning transmission electron microscopy in scanning electron microscopy (STEM-in-SEM) is a convenient technique for soft materials characterization. Various specimen-holder geometries and detector arrangements have been used for bright-field (BF) STEM-in-SEM imaging. In this study, to further the characterization potential of STEM-IN-SEM, a new specimen holder has been developed to facilitate direct detection of BF signals and indirect detection of dark-field (DF) signals without the need for substantial instrument modification. DF imaging is conducted with the use of a gold (Au)-coated copper (Cu) plate attached to the specimen holder which directs highly scattered transmitted electrons to an off-axis yttrium-aluminum-garnet (YAG) detector. A hole in the copper plate allows for BF imaging with a transmission electron (TE) detector. The inclusion of an Au-coated Cu plate enhanced DF signal intensity. Experiments validating the acquisition of true DF signals revealed that atomic number (Z) contrast may be achieved for materials with large lattice spacing. However, materials with small lattice spacing still exhibit diffraction contrast effects in this approach. The calculated theoretical fine probe size is 1.8 nm. At 30 kV, in this indirect approach, DF spatial resolution is limited to 3.2 nm as confirmed experimentally.

scholarly journals Pterygodermatites (Multipectines) affinis (Jägerskiöl, 1904) (Nematoda: Rictulariidae), a parasite of Cerdocyon thous (Linnaeus, 1766) (Carnivora: Canidae) from the Brazilian Amazon

2020 ◽  
Vol 29 (3) ◽  
Author(s):  
Naida Paola Araújo da Silva ◽  
Ana Rita de Lima ◽  
Érika Branco ◽  
Raul Henrique da Silva Pinheiro ◽  
Elane Guerreiro Giese
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Brazilian Amazon ◽  
The Body ◽  
Bright Field ◽  
Wild Mammals ◽  
Bright Field Microscopy ◽  
Cerdocyon Thous ◽  
Morphology And Morphometry ◽  
Scanning Electron

Abstract Among the Nematodes of Cerdocyon thous, the genus Pterygodermatites is characteristic for presenting cuticular projections along the body, being mentioned in different hosts in South America, although there are no records of its occurrence in the Amazon Biome. To enable further exploration in terms of their morphology and morphometry, the nematodes collected from cadavers of C. thous were fixed and observed using bright field microscopy and scanning electron microscopy, revealing characteristics compatible with P. (Multipectines) affinis, thus contributing more information about the geographic distribution of the parasite, as well as knowledge of the helminthological fauna of wild mammals in the Brazilian Amazon.

Brittle fracture of epidermal cells by liquid shear

1976 ◽  
Vol 20 (3) ◽  
pp. 699-705
Author(s):  
G.M. Gray ◽  
H.J. Yardley
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phase Contrast ◽  
Epidermal Cells ◽  
Transmission Electron ◽  
The Press ◽  
Contrast Microscopy ◽  
Cell Fragments ◽  
Scanning Electron ◽  
Liquid Shear

A suspension of epidermal cells obtained from pig tail skin by trypsinization was subjected to high liquid-shear forces in a French press. The material issuing from the press was examined by phase-contrast microscopy, transmission electron microscopy and scanning electron microscopy. The cytoskeleton of tonofibrils retained the shape of cell fragments, and subcellular organelles remained enmeshed in the network of tonofibrils. Examination of some cell fragments by scanning electron microscopy revealed the internal organization of the tonofibrils. The relevance of these findings to the problem of isolating subcellular fractions from epidermis is discussed.

Hyphal interactions between a mycoparasite, Pythium nunn, and several soil fungi

1984 ◽  
Vol 30 (12) ◽  
pp. 1482-1487 ◽  
Author(s):  
R. Lifshitz ◽  
M. Dupler ◽  
Y. Elad ◽  
R. Baker
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Host Range ◽  
Phase Contrast ◽  
Soil Fungi ◽  
Limited Host Range ◽  
Pythium Nunn ◽  
Scanning Electron

The modes of hyphal interaction between a mycoparasite, Pythium nunn, and several soil fungi were studied by both phase-contrast and scanning-electron microscopy. In the zone of interaction, Pyth. nunn massively coiled around and subsequently lysed hyphae of Pyth. ultimum and Pyth. vexans. In contrast, Pyth. nunn penetrated and eventually parasitized hyphae of Rhizoctonia solani, Pyth. aphanidermatum, Phytophthora parasitica, and Phyto. cinnamomi by forming appressoriumlike structures. However, Pyth. nunn was not mycoparasitic against Fusarium oxysporum f.sp. cucumerinum or Trichoderma koningnii and was destroyed by T. harzianum and T. viride. These observations demonstrated that Pyth. nunn was a necrotrophic mycoparasite, with a limited host range and differential modes of action among suscepts.

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