Chemical and Sensory Assessment of Roundnose Grenadier (Macrourus rupestris) Subjected to Long Term Frozen Storage

1982 ◽  
Vol 47 (5) ◽  
pp. 1670-1674 ◽  
Author(s):  
J. R. BOTTA ◽  
A. P. DOWNEY ◽  
J. T. LAUDER ◽  
M. O'NEILL
Keyword(s):  
Frozen Storage ◽  
Sensory Assessment

Evaluation of long-term frozen storage of plasma for measurement of high-density lipoprotein and its subfractions by precipitation

1990 ◽  
Vol 36 (5) ◽  
pp. 783-788 ◽  
Author(s):  
M N Nanjee ◽  
N E Miller
Keyword(s):  
High Density Lipoprotein ◽  
Liquid Nitrogen ◽  
Frozen Storage ◽  
Density Lipoprotein ◽  
High Density ◽  
Long Term Stability ◽  
Long Term Storage ◽  
Different Temperatures ◽  
Edta Plasma

Abstract The concentration of high-density lipoprotein cholesterol (HDL-C) in plasma is now established as an independent risk factor for coronary heart disease, but more data are needed on the relative risk-predictive powers of different HDL subclasses. For epidemiologic and clinical purposes, isolation of HDL from other lipoproteins and separation of its two major subclasses, HDL2 and HDL3, are performed most conveniently by precipitation. Although storage of plasma is commonly necessary, little information is available on the long-term stability of HDL subclasses at different temperatures. Therefore, we quantified HDL-C, HDL2-C, and HDL3-C by dual precipitation with heparin-MnCl2/15-kDa dextran sulfate (H-M/DS) in samples of EDTA-plasma from 93 healthy subjects, after storage for one to 433 days at -20 degrees C, at -70 degrees C, or in liquid nitrogen (-196 degrees C). Fourteen samples (15%) were stored for a year or longer. At -20 degrees C, HDL-C decreased by 4.8% per year and HDL3-C decreased by 6.9% per year (P = 0.002 for both variables) relative to results obtained with samples stored in liquid nitrogen; total cholesterol, HDL2-C, and triglyceride did not change significantly at this temperature. When stored at -70 degrees C, none of the lipids showed any change relative to results obtained with liquid nitrogen. Thus, long-term storage of EDTA-plasma at -20 degrees C is unsuitable for subsequent quantification of HDL-C and its subclasses by H-M/DS dual precipitation. Storage at -70 degrees C is preferable, and is as reliable as storage in liquid nitrogen.

scholarly journals Effect of Acidified Versus Frozen Storage on Marine Dissolved Organic Carbon Concentration and Isotopic Composition

Radiocarbon ◽  
2016 ◽  
Vol 59 (3) ◽  
pp. 843-857 ◽  
Author(s):  
Brett D Walker ◽  
Sheila Griffin ◽  
Ellen R M Druffel
Keyword(s):  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Standard Procedure ◽  
Frozen Storage ◽  
Open Ocean ◽  
Field Collection ◽  
Organic Carbon Concentration ◽  
Large Numbers ◽  
Logistical Problem

AbstractThe standard procedure for storing/preserving seawater dissolved organic carbon (DOC) samples after field collection is by freezing (–20°C) until future analysis can be made. However, shipping and receiving large numbers of these samples without thawing presents a significant logistical problem and large monetary expense. Access to freezers can also be limited in remote field locations. We therefore test an alternative method of preserving and storing samples for the measurement of DOC concentrations ([DOC]), stable carbon (δ13C), and radiocarbon (as ∆14C) isotopic values via UV photooxidation (UVox). We report a total analytical reproducibility of frozen DOC samples to be [DOC]±1.3 µM, ∆14C±9.4‰, and δ13C±0.1‰, comparable to previously reported results (Druffel et al. 2013). Open Ocean DOC frozen versus acidified duplicates were on average offset by ∆DOC±1.1 µM, ∆∆14C± –1.3‰, and ∆δ13C± –0.1‰. Coastal Ocean frozen vs. acidified sample replicates, collected as part of a long-term (380-day) storage experiment, had larger, albeit consistent offsets of ∆DOC±2.2 µM, ∆∆14C±1.5‰, and ∆δ13C± –0.2‰. A simple isotopic mass balance of changes in [DOC], ∆14C, and δ13C values reveals loss of semi-labile DOC (2.2±0.6 µM, ∆14C=–94±105‰, δ13C=–27±10‰; n=4) and semi-recalcitrant DOC (2.4±0.7 µM, ∆14C=–478±116‰, δ13C=–23.4±3.0‰; n=3) in Coastal and Open Ocean acidified samples, respectively.

scholarly journals Within-person variability in urinary bisphenol A concentrations: Measurements from specimens after long-term frozen storage

2009 ◽  
Vol 109 (6) ◽  
pp. 734-737 ◽  
Author(s):  
Pablo A. Nepomnaschy ◽  
Donna Day Baird ◽  
Clarice R. Weinberg ◽  
Jane A. Hoppin ◽  
Matthew P. Longnecker ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Frozen Storage

scholarly journals An updated protocol based on CLSI document C37 for preparation of off-the-clot serum from individual units for use alone or to prepare commutable pooled serum reference materials

2020 ◽  
Vol 58 (3) ◽  
pp. 368-374 ◽  
Author(s):  
Uliana Danilenko ◽  
Hubert W. Vesper ◽  
Gary L. Myers ◽  
Patric A. Clapshaw ◽  
Johanna E. Camara ◽  
...  
Keyword(s):  
Human Serum ◽  
Reference Materials ◽  
Frozen Storage ◽  
Metrological Traceability ◽  
Medical Laboratory ◽  
Serum Samples ◽  
Long Term Stability ◽  
Individual Human

AbstractManufacturers of in vitro diagnostic medical devices, clinical laboratories, research laboratories and calibration laboratories require commutable reference materials that can be used in the calibration hierarchies of medical laboratory measurement procedures used for human specimens to establish metrological traceability to higher order reference systems. Commutable materials are also useful in external quality assessment surveys. In order to achieve these goals, matrix-based reference materials with long-term stability, appropriate measurand concentrations and commutability with individual human specimens are required. The Clinical and Laboratory Standards Institute (CLSI) guideline C37-A (now archived) provided guidance to prepare commutable pooled serum reference materials for use in the calibration hierarchies of cholesterol measurement procedures. Experience using the C37-A guideline has identified a number of technical enhancements as well as applications to measurands other than cholesterol. This experience is incorporated into this updated protocol to ensure the procedure will continue to meet the needs of the medical laboratory. The updated protocol describes a procedure for preparing frozen human serum units or pools with minimal matrix alterations that are likely to be commutable with individual human serum samples. The protocol provides step-by-step guidance for the planning phase, collection of individual serum units, processing the units, qualifying the units for use in a pool and frozen storage of aliquots of pooled sera to manufacture frozen serum pools. Guidance on how to perform quality control of the final product and suggestions on documentation are also provided.

Effect of freezing/thawing conditions and long-term frozen storage on the quality of mashed potatoes

2005 ◽  
Vol 85 (14) ◽  
pp. 2327-2340 ◽  
Author(s):  
M Dolores Álvarez ◽  
Cristina Fernández ◽  
Wenceslao Canet
Keyword(s):  
Frozen Storage ◽  
Mashed Potatoes
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