Fatty Acid Composition of Suspension Cell Cultures of Theobroma cacao are Altered by Culture Conditions

Aim. The aim of our study was selection of optimal conditions for the initiation of Amaranthus L. aseptic callus in vitro culture from different types of explants and estimation of fatty acid composition in these types of cell cultures. Methods. In vitro culture using leaf disks and internodes as explants. Inert gas chromatography. Results. The optimized Gamborg medium for callus induction was designed (sucrose 25 g/1L; 2.4-D 0.5 mg/L; NAA 1 mg/L). The kinetin in concentration 0.5 mg/L for internodes and for leaf discs explants were added. The fatty acid profiles of calli cultures from the different types of plant explants were analyzed The highest level of Omega-3 fatty acid were detected in cell cultures from internodes and leaf discs of cultivar Helios. Conclusions. Our optimized protocol for Amaranthus callus initiation could be used for further studies of the synthesis and accumulation of biologically active metabolites in Amaranthus tissue culture. The fatty acid composition of calli cultures depend from explant type as well as from plant cultivar. Keywords: Amaranthus, callus, in vitro culture, fatty acid composition

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A System for Manipulating the Membrane Fatty Acid Composition of Soybean Cell Cultures by Adding Tween-Fatty Acid Esters to Their Growth Medium

PLANT PHYSIOLOGY ◽

10.1104/pp.82.3.771 ◽

1986 ◽

Vol 82 (3) ◽

pp. 771-779 ◽

Cited By ~ 10

Author(s):

William B. Terzaghi

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Growth Medium ◽

Cell Cultures ◽

Fatty Acid Esters ◽

Membrane Fatty Acid ◽

Membrane Fatty Acid Composition ◽

Soybean Cell ◽

Acid Esters

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Dependency of the fatty acid composition of Euglena gracilis on growth phase and culture conditions

Journal of Applied Phycology ◽

10.1007/s10811-014-0458-4 ◽

2014 ◽

Vol 27 (4) ◽

pp. 1389-1399 ◽

Cited By ~ 21

Author(s):

Jan-Philipp Schwarzhans ◽

Dominik Cholewa ◽

Philipp Grimm ◽

Usama Beshay ◽

Joe-Max Risse ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Growth Phase ◽

Euglena Gracilis ◽

Culture Conditions

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The fatty acid composition of sclerotia

Canadian Journal of Microbiology ◽

10.1139/m70-199 ◽

1970 ◽

Vol 16 (12) ◽

pp. 1171-1178 ◽

Cited By ~ 17

Author(s):

J. L. Sumner ◽

N. Colotelo

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Host Plants ◽

Incubation Temperature ◽

Culture Conditions ◽

Natural Environments ◽

Lipid Component ◽

Specific Culture ◽

Fatty Acid Compositions

The fatty acid compositions of sclerotia of Botrytis tulipae, Sclerotinia borealis, and Sclerotinia sclerotiorum produced under specific culture conditions have been compared with those of sclerotia isolated from natural environments. In each organism the lipids of sclerotia isolated from host plants were more unsaturated than those of cultured sclerotia. Fractionation of sclerotial and mycelial lipids of S. sclerotiorum revealed that the neutral lipid component was more unsaturated than the polar lipid component. In cultures of S. sclerotiorum the fatty acid composition of both mycelial and sclerotial lipids was affected by the incubation temperature, becoming more unsaturated as the temperature was lowered, Irrespective of incubation temperature the fatty acid composition of S. sclerotiorum snowed quantitative differences from the "parent" mycelium in that sclerotia consistently contained a greater proportion of oleic acid and correspondingly smaller proportions of palmitic, stearic, and linolenic acid.

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