Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis

2008 ◽  
Vol 37 (3) ◽  
pp. 219-222
Author(s):  
A. E. MONTOYA ◽  
A. L. ALVAREZ ◽  
M. N. MORENO ◽  
A. RESTREPO ◽  
J. G. McEWEN
Keyword(s):  
Paracoccidioides Brasiliensis ◽  
Electrophoretic Karyotype ◽  
Environmental Isolates

Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis

1999 ◽  
Vol 37 (3) ◽  
pp. 219-222 ◽  
Author(s):  
A. E. MONTOYA 1 ◽  
A. L. ALVAREZ ◽  
M. N. MORENO ◽  
A. RESTREPO ◽  
J. G. McEWEN
Keyword(s):  
Paracoccidioides Brasiliensis ◽  
Electrophoretic Karyotype ◽  
Environmental Isolates

scholarly journals Electrophoretic karyotype of environmental isolates ofParacoccidioides brasiliensis

1999 ◽  
Vol 37 (3) ◽  
pp. 219-222 ◽  
Author(s):  
A. E. MONTOYA ◽  
A. L. ALVAREZ ◽  
M. N. MORENO ◽  
A. RESTREPO ◽  
J. G. McEWEN
Keyword(s):  
Electrophoretic Karyotype ◽  
Environmental Isolates

scholarly journals Phylogenetic Species of Paracoccidioides spp. Isolated from Clinical and Environmental Samples in a Hyperendemic Area of Paracoccidioidomycosis in Southeastern Brazil

2020 ◽  
Vol 6 (3) ◽  
pp. 132 ◽  
Author(s):  
Tiago A. Cocio ◽  
Erika Nascimento ◽  
Marcia R. von Zeska Kress ◽  
Eduardo Bagagli ◽  
Roberto Martinez
Keyword(s):  
Paracoccidioides Brasiliensis ◽  
Geographic Area ◽  
Sensu Stricto ◽  
Human Migration ◽  
Clinical Isolates ◽  
Southeastern Brazil ◽  
Environmental Isolates ◽  
Fungal Isolation ◽  
Exon 2 ◽  
Paracoccidioides Spp

Paracoccidioides brasiliensis complex and P. lutzii are the etiological agents of paracoccidioidomycosis. The geographic distribution of these species in South America is still poorly comprehended. Fifty samples of Paracoccidioides spp. were genotyped, with 46 clinical isolates predominantly isolated in the geographic area of Ribeirão Preto, SP, and four environmental isolates collected in Ibiá, MG, southeastern Brazil. These isolates were evaluated by PCR-RFLP (Restriction Fragment Length Polymorphism) of the tub1 gene and the sequencing of the gp43 exon 2 loci. The species P. lutzii was confirmed by sequencing the internal transcribed spacer (ITS) region of the ribosomal DNA. P. brasiliensis sensu stricto S1b (n = 42) and S1a (n = 5), P. americana (n = 1), P. restrepiensis (n = 1), and P. lutzii (n = 1) were identified among the clinical isolates. All the environmental isolates were characterized as P. brasiliensis sensu stricto S1b. The patient infection by P. lutzii, P. americana (PS2), and one isolate of P. brasiliensis sensu stricto S1b most likely occurred in a geographic area far from the fungal isolation site. No association was found between the infecting genotype and the disease form. These results expand the knowledge of the Paracoccidioides species distribution and emphasize that human migration must also be considered to pinpoint the genotypes in the endemic area.

Recognition of Fungal Components by the Host Immune System

2020 ◽  
Vol 21 (3) ◽  
pp. 245-264 ◽  
Author(s):  
Laura C. García-Carnero ◽  
José A. Martínez-Álvarez ◽  
Luis M. Salazar-García ◽  
Nancy E. Lozoya-Pérez ◽  
Sandra E. González-Hernández ◽  
...  
Keyword(s):  
Immune System ◽  
Histoplasma Capsulatum ◽  
Paracoccidioides Brasiliensis ◽  
Sporothrix Schenckii ◽  
Clinical Importance ◽  
Pathogenic Fungi ◽  
Diagnostic Tools ◽  
Future Research ◽  
Host Immune System ◽  
Fungal Antigens

: By being the first point of contact of the fungus with the host, the cell wall plays an important role in the pathogenesis, having many molecules that participate as antigens that are recognized by immune cells, and also that help the fungus to establish infection. The main molecules reported to trigger an immune response are chitin, glucans, oligosaccharides, proteins, melanin, phospholipids, and others, being present in the principal pathogenic fungi with clinical importance worldwide, such as Histoplasma capsulatum, Paracoccidioides brasiliensis, Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Blastomyces dermatitidis, and Sporothrix schenckii. Knowledge and understanding of how the immune system recognizes and responds to fungal antigens are relevant for the future research and development of new diagnostic tools and treatments for the control of mycosis caused by these fungi.

scholarly journals Metabolic Peculiarities of Paracoccidioides brasiliensis Dimorphism as Demonstrated by iTRAQ Labeling Proteomics

2019 ◽  
Vol 10 ◽  
Author(s):  
Danielle Silva Araújo ◽  
Maristela Pereira ◽  
Igor Godinho Portis ◽  
Agenor de Castro Moreira dos Santos Junior ◽  
Wagner Fontes ◽  
...  
Keyword(s):  
Paracoccidioides Brasiliensis ◽  
Itraq Labeling

Biochemical studies on the production of neuraminidase by environmental isolates of Vibrio cholerae non-O1 from Bulgaria

2011 ◽  
Vol 57 (7) ◽  
pp. 606-610 ◽  
Author(s):  
Rumyana Eneva ◽  
Stephan Engibarov ◽  
Tanya Strateva ◽  
Radoslav Abrashev ◽  
Ignat Abrashev
Keyword(s):  
Vibrio Cholerae ◽  
Pathogenic Bacteria ◽  
Environmental Isolates ◽  
Anaerobic Conditions ◽  
Temperature Optimum ◽  
Ph Optimum ◽  
Cholera Vibrio ◽  
Key Factor ◽  
Aeration Conditions ◽  
The One

Neuraminidase is a key factor in the infectious process of many viruses and pathogenic bacteria. The neuraminidase enzyme secreted by the etiological agent of cholera — Vibrio cholerae О1 — is well studied in contrast with the one produced by non-O1/non-O139 V. cholerae. Environmental non-O1/non-O139 V. cholerae isolates from Bulgaria were screened for production of neuraminidase. The presence of the neuraminidase gene nanH was detected in 18.5% of the strains. Тhe strain showing highest activity (30 U/mL), V. cholerae non-O1/13, was used to investigate the enzyme production in several media and at different aeration conditions. The highest production of extracellular neuraminidase was observed under microaerophilic conditions, which is possibly related to its role in the infection of intestine epithelium, where the oxygen content is low. On the other hand, this is another advantage of the microbe in such microaerophilic environments as sediments and lake mud. The highest production of intracellular neuraminidase was observed at anaerobic conditions. The ratio of extracellular to intracellular neuraminidase production in V. cholerae was investigated. The temperature optimum of the enzyme was determined to be 50 °C and the pH optimum to be 5.6–5.8.

Brazilian Red Propolis shows antifungal and immunomodulatory activities against Paracoccidioides brasiliensis

2021 ◽  
pp. 114181
Author(s):  
Lauana Aparecida Santos ◽  
Pedro Luiz Rosalen ◽  
Nayara Andrade Dias ◽  
Julianne Caravita Grisolia ◽  
Bruno José Nascimento Gomes ◽  
...  
Keyword(s):  
Paracoccidioides Brasiliensis
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