An apparatus for freeze-fracturing specimens of dermal collagen in preparation for scanning electron microscopy

1981 ◽  
Vol 121 (3) ◽  
pp. 347-350
Author(s):  
B. G. H. Lamberty ◽  
C. B. Ellis
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Freeze Fracturing ◽  
Dermal Collagen ◽  
Scanning Electron

Frozen Resin Cracking Method for Scanning Electron Microscopy and its Application to Cytology

1972 ◽  
Vol 30 ◽  
pp. 408-409
Author(s):  
K. Tanaka ◽  
A. Iino
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Epoxy Resin ◽  
Osmium Tetroxide ◽  
New Method ◽  
List Type ◽  
Freeze Fracturing ◽  
Tissue Sections ◽  
Scanning Electron ◽  
Intracellular Structure

Until recently, a few reports have been published as to the methods for observing intracellular structure by means of scanning electron microscopy. Makita and Sandborn saw intracellular granules in the glands of the hen oviduct with tissue sections by means of Smith-Farquhar tissue sectioner. Geminario and McAlear applied the freeze-fracturing technique in order to ascertain the intracellular structure of retinas from mice.A new method, attempted by us, embedding the materials in epoxy resin and cracking it into two pieces after freezing, is described below with its application to cytology.1. The pieces of tissue are fixed in glutaraldehyde and osmium tetroxide.2. They are dehydrated by graded series of ethanol and propylen oxide.3. Then they are embedded in small gelatin capsules (size no. 2) which are filled with Cemedine 1500 (epoxy resin) without any catalysts (Epon 812 or Rigolac can be used but the temperature for hardening them is very low, -80°C).

Fine structure of the chorion of Manduca sexta and Sesamía nonagrioides as revealed by scanning electron microscopy and freeze-fracturing

1992 ◽  
Vol 24 (5) ◽  
pp. 735-744 ◽  
Author(s):  
C.C. Orfanidou ◽  
S.J. Hamodrakas ◽  
L.H. Margaritis ◽  
V.K. Galanopoulos ◽  
J.C. Dedieu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Manduca Sexta ◽  
Sesamia Nonagrioides ◽  
Freeze Fracturing ◽  
Scanning Electron

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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